XB-CLONE-3176873
Clone Name: IMAGE:7854947
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Gene:
naxe
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396536 [+]
cttgggcctgctggttgggggggcgcgccttggcgctagggtagcgcaagttggagcccttggcgggacgtgtccgctggggcaggggctggtagcagac
ggaaactcccagtgcaagcaattcagaactggggcagccatggaacggggactgagatacctaagtcaggaagaagcacaggcagtggacgaagagctat
tcaatgaatacaggttcagcgttgatcagcttatggaactagctggacttagctgcgctgtcgctattaccaaggcttatccagttagttcattcacatc
caacttgccaacggtattggtggtctgtggtccagggaacaatggaggagatggtctggtctgtgcccgacaccttaagctttttgggtatgaaccagcc
atacattacccgaagcgcccaaacaagacgctatttgagaacctaacaacgcagtgtcagaaaatggacattccatttgtctcagagtttccaagtgagc
ctgaagtaattgacggagcctacaacctggtagtggatgccgtttttggattcagctttaaaggagctgtacgtgaaccctttggcaatattctgagtac
gttaaagcgcgtcactgtacccattgcaagtgtggacatcccatcaggatgggacgtggagaaagggaacccagaggggatacagccggacatgcttatt
tctttgaccgctcccaaacaatcagctgtgcacttcaccggacgataccacttcctgggtggaagatttgtcccanaggcactagagaaaaagtacagtc
tcaatctcccaccataccctgggactgaatgtgtccagaagctgccttanaccaaagagtgtgcttagg
3' EST: DT396535 [+]aaaggcatgatttattgaatatacatgttacccaatgacagctttagaaaggttaagggtatggacttatagatacaataattatgctcaaggtgctttt
ctccttattttattaaaggttaagtacttattttttatagcaacaagaacagcaatgaattacctgatttttgcctgatttggccaccccaaatgccaat
aaagtcctctctcaattcaatttgcaagcctgccaatagattgcagcgctgtggaatatgttggggctttataaatacatgttaataataatatctggca
ggccgtcacctccatacaccgtccaataagctgacaacttggtctgaagtggctgagacagcagcttatatcaacctactcataaccaacttaactctca
accttccatgaaataaatgggaaagtagttccctaagtgctataaggccacaagttgatcatctgatctatatcatgctgcatttaaaccaatcctatgt
ggttttctgggtgatgtggtagtaaatatacttggacaaaaaagtgccctatcttaatttgtaatttcaatttttcagtcactgcctactgaattccacc
ctcccctaagcacactctttggtttaaggcagcttctggacacattcagtcccagggtatggtgggagattgagactgtactttttctctagtgcctttg
ggacaaatcttccacccaggaagtggtatcgtccggtgaagtgcacagctgattgtttgggagcggtcaaagaaataagcatgtccggctgtatcccctc
tggggtccctttctcacgtcccatcctgatgggatgtccacacttgcaatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: