XB-CLONE-3176770
Clone Name: IMAGE:7854813
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396604 [+]
ccagctttgcatttctcctcttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtg
caaagccggctttgctggagatgatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccagaag
gacagctatgtaggtgatgaagcccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatggaga
agatttggcatcacaccttctacaatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacagaga
aaagatgacacagggctctgtacctctcctggcatttccctcctgaagtctcagatcatgtttgagactttcaacaccccagctatgtatgtcgccattc
aagctgtgctgtctctgtatgcctctggtcgtaccactggtatcgtgatggactctggagatggtgtcacccacacagtacccatttatgaaggttatgc
cttgccccatgctatcctgcgtctggacttggctggtcgtgacctgacagactacctcatgaaaatcctgaccgaaagaggctacagcttcaccaccaca
gctgagagagaaattgtgcgtgacatcaaggagaaactctgctatgttgctcttgactttgaacaagagatggcaactgctgcatcctcctcatccttgg
aaaagagctacgaactgcctgatggacnaagtattacaattggaaatgagaggtttagatgccccagagcactcttccagccttccttcctgggtatgaa
t
3' EST: DT396603 [+]ggaagtacaattctcatctggatgacatacaaattttggttttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaacttgga
tgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggttctt
gggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtattaa
gcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaagtg
tcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaaccaggc
aatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatcccccaaca
gtgactatgtgtgtcttcaattcctaggataagttcatttggaagttggccctttttccagtttcaagaggctagcatgaggcgtacacatttgccaagg
caaattaatactttgaagaactcacgcagcaaatgcaacgcatctgctagcagtccatttagaagcatttgcggtggacaatggatgggccagattcatc
atactcctgcttgctgatccacatctgctggaaagtggagagggaggccaggatagagccaccaatccagacagagtatttgcgttcaggtggggca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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