XB-CLONE-3176621
Clone Name: IMAGE:7854969
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Gene:
agtpbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396702 [+]
tcttatattacctttagacaaatacaaagagacaataaataaaatggtttggtggacctttaaaagagaatgacaatactgttgagtaaacatcaaaaca
tgaagcagaatcaaacatacaataaagactattggtacattataatggatatttttctaaatctgattatttatattgtaagctctttggagcaggaagc
ttctttatcctgcatctcctaatgtatgtacttaatctttgtaactgtatatatttattgaactgtttgttccctacctgtactattaatgtattgtaaa
aatgtacaacactgcatatacagtggtgctgtataagtaaagatatacatacatacaaaaacaaataagttaatttttgacgacagtccaacctaggcac
cgcctgaccattacatttaatttctgcaagcaaacagttactagacgtttaaaagtttttaattcccaagaacaaaaggaatatatggagtagttgttat
ggtttctttttgggtaacacagtatttcattttgggcttcagtacacacagaacgatcacagaatcaagttaaagcaagatctgaagaaaggtcatgcaa
tataaacaaagaaaataattggtaacctctcttatgggtctataacttacaaattaagtagcaaatgacaagtttgtaaagccatcaaaagcctgctacg
catttattgatttacagtacttaaataagcttttggtattttccttatgaaggcagaaaagaagccaatgtccaacaagtatacgcagctgcacaaccaa
cacatcacacactcctgcaatggtggttcagaccccctagatgaagagcataagtcatagactgtaacatacagtattgaaggagc
3' EST: DT396701 [+]gacaacctatttttacaatagatgtggtaagctcactgcttgcatgttatataaagtgtttctggctccttcaatactgtatgttacagtctatgactta
tgctcttcatctagggggtctgaaccaccattgcaggagtgtgtgatgtgttggttgtgcagctgcgtatacttgttggacattggcttcttttctgcct
tcataaggaaaataccaaaagcttatttaagtactgtaaatcaataaatgcgtagcaggcttttgatggctttacaaacttgtcatttgctacttaattt
gtaagttatagacccataagagaggttaccaattattttctttgtttatattgcatgacctttcttcagatcttgctttaacttgattctgtgatcgttc
tgtgtgtactgaagcccaaaatgaaatactgtgttacccaaaaagaaaccataacaactactccatatattccttttgttcttgggaattaaaaactttt
aaacgtctagtaactgtttgcttgcagaaattaaatgtaatggtcaggcggtgcctaggttggactgtcgtcaaaaattaacttatttgtttttgtatgt
atgtatatctttacttatacagcaccactgtatatgcagtgttgtacatttttacaatacattaatagtacaggtagggaacaaacagttcaataaatat
atacagttacaaagattaagtacatacattaggagatgcaggataaagaagcttcctgctccaaagagcttacaatataaataatcagatttagaanaat
atccattataatgtaccaatagtctttattgtatgtttgattctgcttcatgttttgatgtttactncacagtattgtcat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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