XB-CLONE-3176618
Clone Name: IMAGE:7854635
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Gene:
atg13
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396704 [+]
tttttttttggaaagacatagatttattatatagtacatatctgaattacatctctcttcccagtggatgcaatgggtgtgagtagcagcatagaaacct
cctaaggtagtgtatactgagagccaacctgtagcctgcggctgagctgctgcagaacatctttatcctcctgtcacatctgcaaggggcatatgctgct
ttattgaactacaactcccatcagcctctatgcaagcaatagttctcttttacacagttacagtaccagttccaccagggcagggttatgaaacggacca
ggaaggaagggctactcgttagctaacgaggctgcacagttctgcttttgcacacaggcagggtgggatggactgggcactaatgcacaatatgatttgt
atggggatagggatctctttctatctgacacccacaaggcctattcttatacagactaggggccagcacagagacagcttttgcccggagccctcagaag
gggccccttcattagaaaatgataaatgtatatacaacatagtgcaattactattcatttgtacttgggataagaaattacatagcaatcatacagcagt
tcaactacaacttccaacacgccacatcgtttacactgagggttgtagcccaacattttgggggggtcctaaagtccaagatatgttattgtagaagaat
accttttgcttgtttcaaggatactggttccggcggaggaaatagaaatcatagagctggtgcttttgtgccagcgctgtagattctatgtgcacggccc
ataaagggttaaaggggttgtaaacccagcctagatgccaaaaacatatatagatgcaagaaaaatttcaccatgagaaatctactgataaataacttca
ttataa
3' EST: DT396703 [+]gcagaccgtcagaagcaggtaagtacttcaggtagattagtaccatgcagctatttccccagcggtgtacccagaacatgctggccccccctgcaaaaaa
aatctttagaggggcccaggtacctagccaccacccctccccacccactcatacctgcacactaatgttatgcctgcccgctgcttaatatagccactgc
atttcccaaagggtatgtgttcagatcagtgtcttggaaaacaaatcacagttgttgcaggcacagctatgcttccactgcactactaaatgctagcatg
gtaactatggccttccaggttaaactttctgtttgatttgcgagaattgtgttgtttacattttcatgtactctccccgttttttctgcattaatctcct
tacttggcgcttgtttgaaatgtcctcacaggggcgctgaagagcaagtgcttacctaccctgcagtggaggagtctcaagaagtctgcacaacatcgtt
ttccacttcccctccttcacagatgatggcaccaggaaaagaaagcggtttgcctgctgttagtgttcagcctgcccacggaaaccaggcagagcatgac
cggaccctgtctgtgtctcccgccaatgtagcagggacaacatccagcagcgaggaagcagactccccctcaggtagcagcaccactggggcaaaaagct
cgcccaacaagaacacccatgtactatttacaaggaaagtgggagcttttgttgataaaccttccccccaggtcacctatgccagtactgatataccgtt
ttgccgtgtttgtgccaaaacttgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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