XB-CLONE-3176603
Clone Name: IMAGE:7854923
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396714 [+]
gatcatgcctgcattccaataaagaaatctgatccagtcgtatcttaccgtgagactgtgagcgaggagtccaaccagatgtgcctgtcaaagtctccta
acaagcacaaccgtctgtttatgaagtgtcgaccttttcctgatggtcttgctgaagacattgataaaggtgatgtatctgctcgccaggagttgaagac
tcgtgcccgctacttggctgaaaagtacgaatgggatgtgactgaagctaggaagatttggtgttttggtcctgatggctctgggcccaacatacttact
gatgttactaagggagttcagtatctgaatgaaatcaaagacagcgttgttgcaggattccagtgggctacaaaggagggtgtcctgtgtgaagaaaatt
tgagaggtgttcgttttgatgtccatgatgtaacacttcatgctgatgccatccatagaggaggaggacagatcattccaactgcacgccgcgtcttgta
tgcttgtgtgttgactgctcagccacgtctgatggaaccaatctaccttgttgaaattcagtgccctgagcaggtggtaggaggtatctatggtgtcttg
aacagaaagcgtgggcatgtctttgaagaatcccaggtggctggaacaccaatgtttgtggttaaggcatacctgcctgtaaatgagtcatttggtttca
ctgctgacttaagatcanacactggtggccaggccttccctcagtgtgtgtttgaccactggcaaattctacctggagatcctttcgataactccaccag
gcctttccaagtggtggcagagacc
3' EST: DT396713 [+]aacagatcaattaaatttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaa
attcacatttacttccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattat
attcagggttccaacagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttac
agcttgtcaaggaagttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcga
aaggatctccaggtagaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactc
atttacaggcaggtatgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagata
cctcctaccacctgctcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtg
cagttggaatgatctgtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcaaattttcttcacacag
gacaccctcctttgtagcccactgggatcctgcaacaacgctgtcttttgattcattcagatactgaactc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: