XB-CLONE-3176541
Clone Name: IMAGE:7854881
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396755 [+]
cagggaaagaatattggaaaatacacaattaaagcaaagaacttccatggacaatgctctgccacagcatccttaaatgtgcatcatcttatggaagaac
ctgtcaagcaggttgtattacagacagctggggaagtcagccttcaggaaggattctccttccaggcaatgaaaatgtcttcttccaggcaagaggcctc
cttcagcagtatctccgagacaaagagcatgtcctcaaaaagtgaggcatcatttgttgaaatgagttcaagtagctttatggaaaaatccagtctgatg
cagagttcatctagcagaatgcttacagctggtttcagaggaatacctcccaaaattgaagctcttcccaatgatgtcagcattgacgaaggaaaagtac
ttactgtggcttgtgccttttctggagaacccacaccagatattgcatggtctaaggctggacatccagttcccactcaagaacaaaaaggcagagttca
cattgaaacttctgaagacttaaccactcttatcatcatggacattcagaagaaagatggcggacattacactcttaacctgtcaaacgacttcgggtcc
gattctgcatctgtgaatattaatgtccgaacacattaagcattttatcgtttacattcacatgtaattttacaagtgattcactagaactagatgaact
gatttgtaaatataaaagctatttttgtacctacctgtataagacaaagtcgaaaagatttacgctatcacatattcctatttgaatagggaaattgata
catttttaactgacatgtacatactgtatatagccgagtttaccggttatggagttctgtaccatatattttatgacgttttaaaatgaaactggaacca
acggtgggca
3' EST: DT396754 [+]ttaatggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaata
atgacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccct
attctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccagg
tgatgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacataca
aaagttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaata
ttcacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctg
ctgaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggtt
ccagtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatca
atttccctattcaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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