XB-CLONE-3176467
Clone Name: IMAGE:7854649
|
Gene:
ankle1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT396804 [+]
attttgaccaagcagcaaatcagtcccagtaattttacatccacatgtggaacccaaatccagatttatgattcagacactcttccaattgctagtcaga
tacatctgaatgggaaagaaaacaaggtactggatgggcaattaaaagatatgttgtttttgacgaagcaacaagttatttcctctaatttcattgctac
atctgacacactttcagtagaaccaggtacagaagatgtgcatgataatgctgatccagaacaggaatttattaagcagctgaaagatatgatgttttta
accaagcaacaagtcagtcactgtaattttacacccatccctgaaacacaaactgagggatatgattcggacactcttccagttaccaatcagacaagtc
taactggggaaaaagccaaggtactggatgggcaattgaaacatatgatgtttttgaccaagcaacaagtcagcccctctaatttcttggctacatctga
cacatttgtagtggagtcaactgcagaagatgtgcaagataatagtaatgccgatctaaacacagagctgaggaaaataatgatgttaactaagacttcc
cagtctcatccagatcaggaaaaaagcttttgccatttcactcctcgtacaaaaagccgtcttctttcttccagttctcgctctagcagttcactttttg
atgagacagtggaaatgccccagcgaggaagaagaatccgaagcccagatgctcaaaagtctccgtatgggtcagccatgagtggctccaggagaggttt
gtttcattgtagcttggcatgtgaccaagca
3' EST: DT396803 [+]ttgagactaagagctttattataaaacaaacaaaaatagacaaagaaaaaaacatacaaaacagtaggtataaaaaaaaatctatggatgaaaaatgttg
agagatacagatagaagttgggttactggcaaacttggcaaatagatggttatgccactggactgaattcacttggtttcaacagtggaaaaactgcatc
atctatttcatgaatatgctgacctcagtttgcatttggctttttttttaacattcgtcaataaataatttataataattcattttgtattaattttact
gttgtcatttattaagaaagaaggtattttcaaattagctgtaggtactccctacctaagaaacatttggactttccaaacgtattaaaaattgatttta
catttgttatatttgcactgaattcctcaattaatacctagttgtcataaatgccaattcatttataaaagttaatactatgcaactgaaatttttcagg
acaaaactacacttcaaaaaccacctctaaaggattggaaatattctagaattctttatatacaatattctacaattccaagtataaaattgagtgttag
aaaatcagttattttccttgctgatttacagatcttagaaaggaaacggaaaggtataaatggcgggttgtttgttagacacccccccaccagtgattta
aattggttactgaagaccgtgggacagtgctcctgctaacaactgtgctgccctgtggtactagtttaagagctttgcaccacagttatctttatcgttc
aaaatcttcactacgttccagtcgtggtgcaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: