XB-CLONE-3176295
Clone Name: IMAGE:7854714
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Gene:
rplp0
,
nsmce3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396918 [+]
gcaatccttggaagcactgacaagatgcccagggaagacagggctacctggaagtccaactatttcctgaaaatcattcaattgctggatgattatccaa
aatgcttcattgtgggggcggacaatgttggttcaaaacaaatgcagcagatccgtatgtccctgcgtggaaaagctgtggtgctcatgggaaagaacac
aatgatgcgcaaggctattcgtggtcatctggaaaacaacccagctctggagaaacttctatctcacatcaagggtaatgtgggctttgttttcacaaag
gaagatcttactgaggtcagagacatgttgctggcaaataaggttcctgcctgtgcccgtgcaggagcaatagcaccttgcggggtcgttgtgcccgctc
agaatactggattgggccctgaaaagacctccttcttccaggctttggggatcaccacaaaaatctccagaggaaccattgaaatcttgagtgatgtgca
gctgatcaagactggagataaagtgggtgccagtgaagccacactcttaaacatgttgaatatttccccattttcttatggattaatcattcaacaagtg
tatgacaatggcagcatctatagtccagaggtgctggatatcactgaggaggccttgcatgttcgcttccttgagggtgttcgtaatgttgccagtgtct
gtcttcagattggttatcccaccgtggcctctgtgcctcactcagtcatcaatggatacaagcgtgtccttgccattgcagttgaaacagactacagctt
ccccctggccgacaaggtcaaggcattcctggcagatccatcagcctttgttgttgctgctcccgctgctgacgtggctgctgctcctgctgctgctcct
gct
3' EST: DT396917 [+]catttcatgggctgtttttatttcaagtggttacacatctccaggagatttggactttgttctgcaacactgatgtcttaaagtgacagttaaacacatt
aaacttgaacgttctgttcacaataactgctggttcagctaaaatgccatatgcttgctgttgcttttccaagcactgcagggaatctcagctcagacta
cttattgtcaggaagagaagttcattttcatgttttaataaaatactatttctttgcaaaaagccctttgtgctgctttccttttaatttccactgagcc
cattcattcatattctagcagcacaacccctgctccagtgatggataatttgcaccctgcatcctcctaaaaaggaggtgactaaatgtaccacagggac
actaacatatgactgtcctccagataccagagtttataaaattatattaaaaaagtcactaaacatgattaaaaataaacagacccaattactaaatata
caaacaaatcactaagctgggcctgagaactaccttttagctaccttccaataaagcataactggggctctgcctatatgggggaatagtagccacaggc
aaaaaaaaaaaaaaaaaaattaaccccactagccattccctccattgtacatccttactgcattcccagtttggcgcctcattagccactgacacttccc
tcatgttaaaaatcaatctgattggagcaactctaaaacaaaattaaaaatatacaaaaaaactttacagttaaaaatgttcgggcacagtgtccaaagc
ttcctctgggtcacgggaaacatcaacattaaatggcttttccctgtgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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