XB-CLONE-3176184
Clone Name: IMAGE:7854820
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Gene:
bcl2l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396990 [+]
atcgattcaattcggccgaggcctggctggcacgctcttccctgggtggcagcgggacccatgtgacagtcgggctcacccaatgcctctcgggcctgac
catggagggcagcagtagagatctggtggagaagtttgtttgcaagaaactgtcccagaaaggagcctgcggggagttctccagcaactcccagcccaag
ggcgtgtctaatggaagttcggagggccccggggccacacagggcattgtgggggaggaagtccttcaggcgctgctggacgcgtcggaggagtttgagt
tgagataccagcgtgccttcagcgacctgaccgcccagctgcacctcacccaggacactgcccagcaaagcttccagcaggtggtgggggagttgttcag
ggacggcaccaactggggcagaatcgtggccttcttctcctttgggcgggccctgtgcgtggagagtgccaacaaggagatgactgagctgctccccagg
atcgtgcaatggatggtgcagtacctggagcatacgctgcagccctggatgctggagaacggaggctgggaagcttttgtcggtctgtacggaaagggag
ctgccgcccagagcagggaagggccggagcggtttggccggtggctaatggccatagtgactgtgactgctgcaaccttattggtctcctacctgaggcg
tcgatagacccccagaagttctgcggtgatgtgctattggctggcactggccccacccatggacacgataggctggtggctcccaggttcaacatgtgtt
gaggttccctttgctcagttacagggagatggag
3' EST: DT396989 [+]aaacataacatttaataaccactgctgagcgcaaggcaataacagacaacctaacacagaggttccccccctccccaggaggttcccaagttgtggggtt
ccccccctccccaggaggttcccaagttgtggggttcccccctccccaggaggttcccaagttgtgggggttcccccctccccaggaggttcccaagttg
tggggttcccccccttcccaggaggttcccaagttgtggggttcccccctccacgggaggggcccatgagattattggggtgaatacaccggggctccat
ctccctgtaactgagcaaagggaacctcaacacatgttgaacctgggagccaccagcctatcgtgtccatgggtggggccagtgccagccaatagcacat
caccgcagaacttctgggggtctatcgacgcctcaggtaggagaccaataaggttgcagcagtcacagtcactatggccattagccaccggccaaaccgc
tccggcccttccctgctctgggcggcagctccctttccgtacagaccgacaaaagcttcccagcctccgttctccagcatccagggctgcagcgtatgct
ccaggtactgcaccatccattgcacgatcctggggagcagctcagtcatctccttgttggcactctccacgcacagggcccgcccaaaggagaagaaggc
cacgattctgccccagttggtgccgtccctgaacaactcccccaccacctgctggaagctttgctgggcagtgtcctgggtgaggtgcagctgggcggtc
aggtcgctgaaagcacgctggtatctcaactcaaactcctccgacgcgtccagcagcgcctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: