XB-CLONE-3176173
Clone Name: IMAGE:7854630
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396997 [+]
gcctacgacccaagcgctaacgaatgcttcttcgcctcactctccgcccagatccccaaaaaccacgtcagcctggccactaaggagaaccaactctttg
tagctggcggcctttattataatgaggaaaacaaggacgacccactgagctcttacttcctgcagttcgatcacctggattctgattggctgggaatgcc
ccctgtcccctcagcacggtgcctgtttggtctcggggagtcggacaattccatatacctgattggtggaaaggaactaaaagaaggagaacagacgctg
gacactgttctatgctacgaccgtccgtcatttaaatggggagagtcggacccgctaccctatcaagtgtacggtcacaccgtggtgtcccatgataatc
tggtctacgttcttggaggcaaaggaaatgaaaagaaatgtttaaaaagagtttgtgtctacaaccccaagaagtttgaatggaaggacctagcaccaat
gaaaaccgctcggtcgctattcggaagcactgtccacaagggaaagatcttaatcgccgccggagttacagacacgggtttaaccaacaccatcgaagcg
tatgatgtcaagaccaataagtgggaggagttcacagagttcccccaggaacgtagctccctcagcctcgtcagcatgaacgggaccctttatgctattg
ggggatttgccaccacggagaacgaatcgggggagtttgttccgacagaactgaacgacatatggaggttcaacgaagaggagaagaaatgggaaggaat
tcttagagagattcgatatgcgtctggagcgacccttcgtgcagcgcggctcaatatcctgcgactaacgaagatgtaacgacgcgccaggc
3' EST: DT396996 [+]cagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaatcct
tgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgaccttta
ttgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaatttagg
tacctgccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttgaaaa
tcatttagcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaatgg
ttgcatatgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaagatt
catccgaatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatgatat
aatctgtgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtagaaatgacaggga
gttgatagctacaaactgtgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: