XB-CLONE-3176111
Clone Name: IMAGE:7854636
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Gene:
pjvk
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397038 [+]
ctgttattgggctgctgatactttgggctggtacagttgttcgctacataacgtacagcatttctatcatgtttatctttaggatttagttctcctttaa
aggtgttagtaaaagatttacataagaaacctcactggagatgggggtggagtctatatgatatttctgcatgtttcccttcactttatacttatgggaa
caaaaggagagatgtatttactagtcagctgcaaagactggacactaatgtgcccggctcctcatgtattggccaatgactctgtggcagttaatgcacc
catgcttctggaagctggcggttttctgggaccagcccttgcagatcattagcaaacacgtttcttgggttttttttccccagtgtagaggttgggtcac
tattctatcaccaacaaggtctcactatatggggccggctgaaggtgacagactggccattccagatgtatatctgcaccaataacatccagcttttcct
tgctcctccgagcagcagtaaatgctttgcatcataagcccattggatggaagcgggttcttttaaacgcttataagaaggaagcgacaccggtatgata
gaagaatttatttttgtattcggctcctgcaagaattgatctgatccacacgtcagagtatgtaagcttgtgtacatagacattttatattctattcctt
aatatgatgatgtatgttaatagtttcagatgttttatcggtcactgtgaatgtgttanatcctatttgctggaacttcagatacgttgtgtgtgtgtgt
ccgtcagcc
3' EST: DT397037 [+]ttttggtaacaagtctgtattttacctttgtaaataatacatttcttatatgaaaaagcaacacttttgacaaccaaaatagcttccttggggcaaaatg
ttgttctctggctgacggacacacacacacaacgtatctgaagttccagcaaataggatttaacacattcacagtgaccgataaaacatctgaaactatt
aacatacatcatcatattaaggaatagaatataaaatgtctatgtacacaagcttacatactctgacgtgtggatcagatcaattcttgcaggagccgaa
tacaaaaataaattcttctatcataccggtgtcgcttccttcttataagcgtttaaaagaacccgcttccatccaatgggcttatgatgcaaagcattta
ctgctgctcggaggagcaaggaaaagctggatgttattggtgcagatatacatctggaatggccagtctgtcaccttcagccggccccatatagtgagac
cttgttggtgatagaatagtgacccaacctctacactggggaaaaaaaacccaagaaacgtgtttgctaatgatctgcaagggctggtcccagaaaaccg
ccagcttccagaagcatgggtgcattaactgccacagagtcattggccaatacatgaggagccgggcacattagtgtccagtctttgcagctgactagta
aatacatctctccttttgttcccataagtataaagtgaagggaaacatgcagaaatatcatatagactccacccccatctccagtgaggtttcttatgta
aatcttttactaacacctttaaaggagaactaaatcctaaagataaacatgatagaaatgctgtacgttatgtagcgaacaactgtaccagcccaaagta
t
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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