XB-CLONE-3176108
Clone Name: IMAGE:7854684
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Gene:
atg14
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397040 [+]
attttgcatgatttgcctggtttcccccattttgctcaaggagaattttgcagtgtggcagttactgctaagcacagtaaatgtctgatgatggccattc
ttgggccgacccgtacattacatcacagtaagcccgtggctgaaggaagcttagtaaataagtagcaatgcagatgtgaggccacttttctgttctttcg
attgaaacagcaggaagtgcacagaagctgcatctcctctcctttaagatcataaataatcctccatccaccaagactaagattttctttccaaccagcc
ctctgactgtgcaggccctagctgttctagcactaccagccagccatgctttgggttgtccataagcagtaggtggaatgcaacaggcactgaacactca
gttattattctatgctgttataatgctttacccctgtacaacgtctgccttaactaactagtagcactcagtagatattgaaactaggaataatttgtgc
atcagcactgtgctaaatctggcttctaggagtcatgttaatgccagttattggctttatggttggcctggtattaggtatgttattctgcctgtgcctc
tgcttggtattacctggcaataaggagaatccttctgagtcaatagaagctgcccatcataatagcggttgcagcatgtctctcataagaatatggtgca
aacttgcatgtcatatttagatatttgttttgatttgtgtaatccacatatttaatttctccatgatgtatagctgtaatgtgcttaaaccaatggatac
agttgatggggaacactaagtggaggggccttctgtttagcagcaggtttagtggatgtctgcacggcgcancgtgcttgt
3' EST: DT397039 [+]aataaaaaatagtcaactgtttttatttttataatgcagacgttcctagcatcatacagtgttgaactgactgtcattcatttcagtttgctctcagaaa
gtgctaaatatttaaaaaaaaaaagaaaagaaaaagcacaggcatggtaaaataaacacaatataaatacacatcttgctataggcactctctttaagga
tggaggcaatgagcataaagaggaataaagactattaactagatcattctttgctatactcggttccttatgccacatacaaatgtcaccaccgtatact
gagaagaatttactattattcatattattacacgttggaaatgaaacaggccagccctcccacaagcacgctgcgccgtgcagacatccactaaacctgc
tgctaaacagaaggcccctccacttagtgttccccatcaactgtatccattggtttaagcacattacagctatacatcatggagaaattaaatatgtgga
ttacacaaatcaaaacaaatatctaaatatgacatgcaagtttgcaccatattcttatgagagacatgctgcaaccgctattatgatgggcagcttctat
tgactcagaaggattctccttattgccaggtaataccaagcagaggcacaggcagaataacatacctaataccaggccaaccataaagccaataactggc
attaacatgactcctagaagccagatttagcacagtgctgatgcacaaattattcctagtttcaatatctactgagtgctactagttagttaaggcagac
gttgtacaggggtaaagcattataacagcatagaataataactgagtgttca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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