XB-CLONE-3176097
Clone Name: IMAGE:7854876
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397047 [+]
ccggccagcaggaatgtcccagccacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcc
caccaggtcccaatcaaatccaaaacataccaggttatgcaggagtgcgaacaatctggaacaacagccccttctgtgttcagccagaacaggacgggcg
gagaaacagcctttgacaagccaaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatt
tttgttcccaccttctactcattgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagtta
ctgtaagactttatttcattgctcctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctctt
cagttattaagtcaacaaaggagccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaa
cccttacaggtccttaggctttggctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcaccta
ctccccatcatcagggtgatatggttcagccctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgaggga
tcacctgctttaaattgtgcattaaatgtaaaattaggacttttcccgttaggtactcatccct
3' EST: DT397046 [+]aaaaccacggaaagtttaaaaaaaacaaaccatataagaacatagcgacacaagaatgatcagggctaatacacaacacacatttacttctcagtgatgc
tgtgactgggtgctctgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcctagtgtgatgccagtggcactgagtt
actacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaactaactggaacacagtaaatctctcttg
ccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattgaatagaaggctacaaggtcaactttct
ctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcctataaaggttgctcagattatcagccc
ttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtgatccctcaccaggcaggaaatggtcta
gtcctgaatccaccttaatatgttgacataggaaaaatgccagggctgaaccatatcaccctgatgatggggagtaggtgatatgactgtgtttaggtcc
ccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctgntagggtttggattttagaaaatacaaattct
gtattcacttcctccattttatatataggaaatctaaaaaatcatatttggctcctttgttgacttaataactga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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