XB-CLONE-3175933
Clone Name: IMAGE:7854987
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397156 [+]
acgaggggacagcagggagctcgaaagggagcagccccaccgccggcctgagttaccatcaacaccccggggggaagttaaagcgagccaaccgccggcc
cgagtcaccacctccgaaagcagccaacttacaccatcaaagacacaatgagcagcgaggttgaaacacaacagcagcagacagtcgcattggagggcaa
ggccggccaggaaccggcggccaccgtgggggagaagaaggtcatcgcaaccaaggttttgggtacggtcaaatggtttaatgtgcgcaatggttacggc
ttcattaacaggaatgacaccaaggaagatgtgtttgtacaccaaactgccatcaagaagaataaccctaggaagtaccttcgcagtgtgggagatggtg
aaactgttgagtttgatgtagtggagggtgaaaagggtgcagaggcagctaatgtaactggtccagggggtgttccagtccaaggcagcaaatatgcagc
agaccgtaatcatttcaggcgctatcagcgtcgcagaggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggagagaagacagaaggg
aatgagagtgcacctgaaggagaggggggttcaaatcagcaacgtccataccgcaggagacgtgttccaccatactacttgcggagaccatatgggcgca
gaccacaatactcaaatgctcctattcagggagaaggtgcagagggatcagatgggcaaggttcttcacaaggtgatcaaggtagacctgtgcgacagaa
tatgtacagaggcttcagaccacgatttcgcaggggaccaccacgg
3' EST: DT397155 [+]gttgagacgtttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaaatctta
gtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagcttttagaaaa
aaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaattctttgt
tcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccgccctgct
cagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtctgtagtt
aaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctgggttgtctc
tgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgaccatctg
atccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcctgcggta
tggacgttgctgatttgaacccccctctccttcaggtgcactctcattcccttctgtctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: