XB-CLONE-3175561
Clone Name: IMAGE:7855117
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397402 [+]
attgctgctcagtattcggggttccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatcagagttcctaaagaaat
tccccttaggcaaggtaccagcatttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggcaatgatgagctccgagg
aaccaatcgtttacaccaagctcaggtcatccagtggctcagcttctcagacagtcacattgtccctccggccagcgcatgggtctttcccactttaggg
atcatgcaatacaataagcaggccacagaacaagccaaggaggagataaaggctgtgctgggtgttttagattctcatctgaagacacggacattcctag
tgggcgagagggtcacgctggctgatatagcagttacttgctctcttctgtggctctataagcaggtcctggaaccatccttccgccagccatttggtaa
tgttacaaggtggtttgtgacctgtgtgaatcagccagaattccgtgctgtgttgggagaagtcaagctttgtgacaagatggcacagtttgatgctaag
aagtttgccgagatgcagcccaagaaagaaactcccaagaaagagaagccagcaaaggagccaaaaaaagaaaaggaggaaaagaagaaggcaacaccag
ctcctgctcctgccccagcccctgaggatgatatggatgaatctgaaaaggctttagcagcagagcctaaatcaaaggacccatacgcacatctacctaa
aagctcttttgtaatggatgagttta
3' EST: DT397401 [+]gatttcagtttttttattttcggtttgctgtcttgttaagttaaaaggctctggatgttggtacggatgtgaaaaccagactctgcgcgtattttagggg
gacagagatcacctcctcctcatttgaagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaagtattctttgaccag
ggttttgcactcctcactattgctgtccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaatgcaaggtcctggccc
ctgaacacccacacgccggagatggagctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgtctagacgctggaaca
tacctgtaattaagttgcagctcatgaaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttccttgtcaaagtgctc
ccagaaataaggcagagctacagccagggtatcctcattagagtattttctcttaaactcatccattacaaaagagcttttaggtagatgtgcgtatggg
tcctttgatttaggctctgctgctaaagccttttcagattcatccatatcatcctcaggggctggggcaggagcaggagctggtgttgccttcttctttt
cctccttttctttttttggctcctttgctggcttctctttcttgggagtttctttcttgggctgcatctcggcaaacttcttagcatcaaactgtgccat
cttgtcacaaagcttgacttctccaacacagcacggaattctggctgattcacacaggtcacaaac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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