XB-CLONE-3175426
Clone Name: IMAGE:7855225
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT397491 [+]
atcgtatttaagccacatggggggtaagtgaggagttatgtgggtatggtctttaaaaaatgtgttaataggggagtggtttgaagtaggtgtggtttaa
aaaagcggagtggccaaaactggcttccattatcagtaccacagaagttggagagcactgccccagggttatcaactgaaaaggttacccaacatgttgg
atcattaaaacaaacacccctttttttgttttgatggggaccactgtatcatatattgctatggaatttaatgttgggaaatacgtgttgtaaatgttac
tcatttagttgggggttcaacaagcttgggtgcggtaaaaccaagtaatcccagaccccccccccacctactgatgaacattgtttccagtagaataggg
ttcattgtaaccaatcagcagtgtcggactggggaaggtggggcccaccattccggcctcgccccgcctccccgctaagggcccacctctccctatcaag
agcacagaggctgagacatgagaaatgaccataatactcccagaaagcagatttttgtatctttattttcctttatcatgtaaaaatctcagtagaatta
gcactgattttgtacgttgagtttgaagattcaaatataaaaatccttgtgcaaccataaatatattgtttcctgtacaaacagaggcctatggggatac
cggtgttgccccgacagccaattaaaagcattagcggctgacctttattgcaggaagtccgtgctggctgggaattctgggagttttagtatagctatag
ataaggaac
3' EST: DT397490 [+]aaattccagcaatgaaaatgagcaattggttgccatgggttactggaccagggcaggtacctaaattccccgatacctatcaaagtaaggatcttcggtt
ccttatctatagctatactaaaactcccagaattcccagccagcacggacttcctgcaataaaggtcagccgctaatgcttttaattggctgtcggggca
acaccggtatccccataggcctctgtttgtacaggaaacaatatatttatggttgcacaaggatttttatatttgaatcttcaaactcaacgtacaaaat
cagtgctaattctactgagatttttacatgataaaggaaaataaagatacaaaaatctgctttctgggagtattatggtcatttctcatgtctcagcctc
tgtgctcttgatagggagaggtgggcccttagcggggaggcggggcgaggccggaatggtgggccccaccttccccagtccgacactgctgattggttac
aatgaaccctattctactggaaacaatgttcatcagtaggtggggggggggtctgggattacttggttttaccgcacccaagcttgttgaacccccaact
aaatgagtaacatttacaacacgtatttcccaacattaaattccatagcaatatatgatacagtggtccccatcaaaacaaaaaaaggggtgtttgtttt
aatgatccaacatgttgggtaaccttttcagttgataaccctggggcagtgctctccaacttctgtggtactgataatggaagccagttttggccactcc
gcttttttaaacacacctacttcaaaccactcccctattaacacattttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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