XB-CLONE-3174094
Clone Name: IMAGE:7855680
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Gene:
pigv
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125799 [+]
CAGAACTGTAAAACAGGAGACAAGGTTGGGTACTAGTTAATGTCTTGGAGCAGCTATGAAATGTGGGCTATCAAAGGATCCATTCCTGCAAGAGATACTA
CGTTTTTCCCTGTGTTGCCGTGGAGCGACACTTGCAATGCAGGTGAGAAATGCTCCTACACTATCCTCTGACATTGATCAGATCATTTTAACCTTTTACA
TGCTGGCATATATATTCTAAGGCACTGGCTCAAACATGCCAGTGGGCCAGCCCCAAGGGTTCAAGGTAAAGAAGAAGGCATTTTCTTATGCATGTGTGCT
GGATTGGCACATATTAAGTGTTTTACAGGTGTCAGTCCCCATAGTTGCACAAGTAAAGGTCATTTACAGCAATTTATCACCTGTTGATTTGCCCTAAAGA
GATGTTACAGCTTTAAAAGATGTAAAACAGATTTAAGTGTCTTAAGCAAGAACACTATCAGTTTTCATGGGGTGCCCTTACTATTATCTCTCTCTCCGGT
AAAGGGAGAGCCATTCATTTGACAACATATTCAGGCATAATGTATTTTCCACAACTTTATGACTATGCCTCTAAATGTAATATATCTTGCTTTCTCTCTT
CTTATTGCTAGGTTGTGTTCAACCTCCTTATCCCAGACCACATTGCTGATGCCTTCTCGCCGCCACGAGCCCCACCTTCAGGTTATGGAGACCAATTTAC
AGAATTGCTACTGGGGGGTCTTGGACGATGGGATGCAGAGCATTTTCTCTTCATAGCAGAGCATGGCTATGTTTTTGAGCATAATATGGCTTTCTTTCCA
TTGTTCCCACTCACCCTAGCAGGTTTAGCCCATGGTTTGCTGTGGCCTCTGGGATTCATGCTTTCCCTGCGCAGCAGACTACTTTTGTCTGCCACTCTTC
TGAATACTGCCTGCTTTGTATTGGCAGCAATTGTTTTGTACCGACTAGGTTGTGTGGCCCTTCAATCACGCCGCTCTGCATTCCTGGCTGCCCTTCTTTT
CTGTATGAGCCCAGCTTCTATCTTTATGACTGCAGCATATTCAGAGAGTCTGTTTGCAATGGCCACCTTTGCTGGACTTTGGCAATTAGAGAAGTGTAGA
ACTTTCCGAGGTTGCTCTCTGTTTTTACTTGCCACTGCTGCTCGTGCCAATGGACTAGTCAATGCTGGTTTCTTGCTGTACTTTGGGGCCCAATCAGTCG
TACAAGGGACAATGAGTTGTAGAAGAATATCTAAAATTTTGTTAGCAACCGCTGTAATAATGCTACCTTTTGCTTTATTCCAGCATTATAGTTTCCACCA
TTTCTGCTTGGTATCTGCAATAGATGAAAAAGTGTCCCCAGAGCTGATGCAGCTTGCGATAGAAAAGAGTTACCGTGTTCGTGGCCCACCAGTCCCAGCA
TGGTGTGCTGCCAGCCTACCATTGGCTTATTCAAACATACAAAGTGATTACTGGGGTGTCGGACTTCTTCGCTATTTTCAACTGCGTCAGCTGCCAAACT
TTCTTTTGGCGTTACCAGTAATCTTGCTAGGTACTCGGGCAATATGGGAGTATGGTTGTGCCAATGTAAATCTATGCCTGACTCTGGGGCTCTGGGAAAA
GCGGCAAAAGTCTTCCAGTGGATATTATGGACCTCGGATATTTGTTTATGTGGCGCATCTTACTGCTCTAATGATGTTTGGAGTCTTCTGTATGCATGTA
CAGGTAAAGGAGTGCAATTCAAGGCTACACTGTGCCAGTAAAAAACATAAATGTGTCAGAGGAAGGGGGTATAGAGATATTTGTTTTTACTGTATTTTGA
ACAGGCACGTACATAGTATGGTAGAAAGTAAACAAAATGGCTGAGGGATATAAGACTGAATGGGCAAGCACTTATTAATAATTTAGTATTTAAACTTCTT
TTTTTCTGCTTTGCAGGTTATCACTCGCTTTCTCTTTTCTTCCTCTCCTGTGCTTTTCTGGTTCTGTTCTCTCCGCCTTCAGCAGAATGAACCATGGATG
TGGGGATCTGAGACAGGCATGTCAGCTTCCAACCCAGCAATTCGATTGCTCCATTCCTGGACGTCACTTCAGCGCCCCACTCGTTCTTTGCTGGGATATT
TCTTGGGTTACTGGGTACTTGGCTCCGCTCTGCACGTCAATTTCCTTCCATGGACCTAGCAGAGAATATAGTGGTTGAGAAAATTAAAGGGAATGTTTAC
TGAAAATGTATCTTAATTTTTTATTTTTATATAAAAGTTTTCAATATCTTACTTTGTGTTTTTTTTTTTTTAATCTTGCAATTTCCTTACAGTATGTCAT
CTTGTTATGGTAACTGGATGTGTGAACAGTTGTTATAGGGATTAAAAAGTTACTTTGATGATTTTTAAAATTACTCAGCAAGTCCAGATGCTGTAGACTC
ATCTCAATTAGATACTGTATATCATGACCTGGACAAATGAAAACTAATTGTTAAATGTTATGCAAGTCAACCCATGTATTCCTTGTTTAATTTTATGTTC
TTATTGACCTGTTAAAAAATATCTATATATTTATATACAGTATATGTGCAAAGTCAAAAGCACTCACAGCCAAATATATCATGACGAAGATCCCTGGAGG
ATCGAAAAGTGTAGAAATGAGTGTGGAGCAATAAATATTTTTTTGCATATATTTTGGCTGTGAATGCTTTCGACTTTGCACATATGCGCTCACCCAAAAA
CATGCAAGAAAGGCCAAGGTGCTGCTGCTAAACATATCAGCTTGTAGGCCCTGACTGGGATACAAAATAGCCCCATCTTACAGCAGCCCCTCTGGCATTT
GCCTGAATCCACAGATTGCCAGTCCAGGCAATAGTATGCATATTTATATATTTATTGTAGTGAAGACCAGCTTGACCAGAATCATGATTTTAAAAGTGAT
GTGTATTTATTGTAGCAAAAATAGCTAGTACCAAACAAAATGCACTGGAACAAGGACAGCACTCCTGGGATTTGGGGAATTGTGAGCGAAGTGTGGGTGC
AGAAACGGAAAGGTTTATTACCCTAGTGATGGGCCTTTCTGTTTTTGCATTTGCATTTTGCTCACGGTTCTTTAAATCCTAGAAGTGCATTATATATATA
TATATATATATATATATATATAAATATATAAAACACTGTCGGGAAATTTGCAAAAAGCAAAGGTAAATGAAAAGTATGCGGCAGGTTTATTAATATTGAA
CTGGGGAATTCCTGAAGGAGTTTTTTTAGCATTTCAATAGTTTTTCCATTTATCAACACATTTGCGACTGTCAGGAAAAGTTTTTAATAAATTGCCATAT
TATCAAAAAAAAAAAAAAAAAA
5' EST: DT398377 [+]cagaactgtaaaacaggagacaaggttgggtactagttaatgtcttggagcagctatgaaatgtgggctatcaaaggatccattcctgcaagagatacta
cgtttttccctgtgttgccgtggagcgacacttgcaatgcaggtgagaaatgctcctacactatcctctgacattgatcagatcattttaaccttttaca
tgctggcatatatattctaaggcactggctcaaacatgccagtgggccagccccaagggttcaaggtaaagaagaaggcattttcttatgcatgtgtgct
ggattggcacatattaagtgttttacaggtgtcagtccccatagttgcacaagtaaaggtcatttacagcaatttatcacctgttgatttgccctaaaga
gatgttacagctttaaaagatgtaaaacagatttaagtgtcttaagcaagaacactatcagttttcatggggtgcccttactattatctctctctccggt
aaagggagagccattcatttgacaacatattcaggcataatgtattttccacaactttatgactatgcctctaaatgtaatatatcttgctttctctctt
cttattgctaggttgtgttcaacctccttatcccagaccacattgctgatgccttctcgccgccacgagccccaccttcaggttatggagaccaatttac
agaattgctactggggggtcttggacgatgggatgcagagcattttctcttcatagc
3' EST: DT398376 [+]gataatatggcaatttattaaaaacttttcctgacagtcgcaaatgtgttgataaatggaaaaactattgaaatgctaaaaaaactccttcaggaattcc
ccagttcaatattaataaacctgccgcatacttttcatttacctttgctttttgcaaatttcccgacagtgttttatatatttatatatatatatatata
tatatatatatataatgcacttctaggatttaaagaaccgtgagcaaaatgcaaatgcaaaaacagaaaggcccatcactagggtaataaacctttccgt
ttctgcacccacacttcgctcacaattccccaaatcccaggagtgctgtccttgttccagtgcattttgtttggtactagctatttttgctacaataaat
acacatcacttttaaaatcatgattctggtcaagctggtcttcactacaataaatatataaatatgcatactattgcctggactggcaatctgtggattc
aggcaaatgccagaggggctgctgtaagatggggctattttgtatcccagtcagggcctacaagctgatatgtttagcagcagcaccttggcctttcttg
catgtttttgggtgagcgcatatgtgcaaagtcgaaagcattcacagccaaaatatatgcaaaaaaatatttattgctccacactcatttctacactttt
cgatcctccagggatcttcgtcatgatatatttggctgtgagtgcttttgactttgcacatatactgtatataaatatatagatattttttaaca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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