XB-CLONE-3172330
Clone Name: IMAGE:7856177
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Gene:
tmem19
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121999 [+]
CTTTTCTGTAAGCGTCCTGGCTTGGGGCATGTAGCGGATAGCATCATTCGCGCTTATTTTTCTGTTTTGTGCAACTGAGATGGATTTTAGCCTTGGGCAC
TTTCCATGTTTTTACCGTGACAGGAGAAATAGCTACCGCTAGCAAAATACTAGGAGAGTGCCAGGTAAACTGTTGAGTCGCTCTCATGCTGTTTATTCAA
GACGACGTATACAAGGAATATGTGAAAATGATGATGAATATTGTCATTTTGTGTATGATCCTCTGCATATCTTTGTCCTTCTGGATAATTTCCATCACAG
CAAGTACCTACTCTGGTTCATTGCGACCAATCTCTCCATGGCGTTGGCTTGTGTCTGTGCTAACCCCAATTATTATTGTATGGCATGGTATAAAGAAGAG
GAGCTTGGATAACAGTGGTGCTCTTGGAGGGCTGCTGGTAGGATTCATCCTCACTATAGCCAATTATAGCTTTTTCTCAGCCTTGCTGACTTTCTTCTTC
ATTTCCTCTAAACTCACTAAATGGAAAGGTGAAGTTAAGAAATGCTATGACTCAGAATATAAAGAAGGAGGACAAAGGAATTGGGTTCAAGTGTTCTGCA
ATGGTGGCCTTCCTGCTGAATTAGCTCTGCTCTACATGATAGAAAATGGACCTGGAGAAATACCAATAGATTTTTCCAAAGAGTACACTGCATCTTGGAT
GTGTTTATCCCTCCTGGGTGCTCTTGCAAGCTCTGCTGGAGATACATGGGCTTCTGAGATAGGACCAGTACTTAGCAAAAGTTCCCCTAGATTAATAACA
ACCTGGGAAAAGGTACCAGTTGGAACCAATGGAGGTGTTACTCCAGTAGGTCTTATTTCAAGCCTTCTTGGTGGCACAAGTGTAGGTGTAGCATATTTTG
TTACACAACTTATTTTTGTACCTGATTTGGAAATCGCAGCCCCTCAGTGGCCCATCGTCATATATGGTGGAATGGCTGGTTTACTTGGGTCTTTAATAGA
CTCCTATCTAGGAGCTATAATGCAATATTCAGGTTATGATGAAAGCACTGGTAAGATTGTTAATCACCCAACAAAAGAGGCAAAATTCATTTGTGGGAAG
CCGATATTGGACAATAATGCAGTGAATCTGTTCTCATCAATTCTAATTGCTCTGCTGCTTCCAACTGCTGCTTGGAGTTTTTGGCCAAGACTTTGAAAAG
TTGGGAAACTATTATTTTCTAATTGTTGAAAAATACATATTGTACAGCAGGCTTGCACTTAATTATTGATGTTTTGTTTTATTGTAGCAGTTATTGTGTA
GAGTTTTAAAGATGTAAACAAATACACTTTTAAAATATAGATGGTTCGGTTAAAATTATTAAATTTTTTCCCCCAAAAAA
5' EST: DT399536 [+]cttttctgtaagcgtcctggcttggggcatgtagcggatagcatcattcgcgcttatttttctgttttgtgcaactgagatggattttagccttgggcac
tttccatgtttttaccgtgacaggagaaatagctaccgctagcaaaatactaggagagtgccaggtaaactgttgagtcgctctcatgctgtttattcaa
gacgacgtatacaaggaatatgtgaaaatgatgatgaatattgtcattttgtgtatgatcctctgcatatctttgtccttctggataatttccatcacag
caagtacctactctggttcattgcgaccaatctctccatggcgttggcttgtgtctgtgctaaccccaattattattgtatggcatggtataaagaagag
gagcttggataacagtggtgctcttggagggctgctggtaggattcatcctcactatagccaattatagctttttctcagccttgctgactttcttcttc
atttcctctaaactcactaaatggaaaggtgaagttaagaaatgctatgactcagaatataaagaaggaggacaaaggaattgggttcaagtgttctgca
atggtggccttcctgctgaattagctctgctctacatgatagaaaatggacctggagaaataccaatagatttttccaaagagtacactgcatcttggat
gtgtttatccctcctgggtgctcttgcaagctctgctggagatacatgggcttctgagataggaccagtacttagcaaaagttcccctagattaataaca
acctgggaaaaggtaccagtt
3' EST: DT399535 [+]aaaaatttaataattttaaccgaaccatctatattttaaaagtgtatttgtttacatctttaaaactctacacaataactgctacaataaaacaaaacat
caataattaagtgcaagcctgctgtacaatatgtatttttcaacaattagaaaataatagtttcccaacttttcaaagtcttggccaaaaactccaagca
gcagttggaagcagcagagcaattagaattgatgagaacagattcactgcattattgtccaatatcggcttcccacaaatgaattttgcctcttttgttg
ggtgattaacaatcttaccagtgctttcatcataacctgaatattgcattatagctcctagataggagtctattaaagacccaagtaaaccagccattcc
accatatatgacgatgggccactgaggggctgcgatttccaaatcaggtacaaaaataagttgtgtaacaaaatatgctacacctacacttgtgccacca
agaaggcttgaaataagacctactggagtaacacctccattggttccaactggtaccttttcccaggttgttattaatctaggggaacttttgctaagta
ctggtcctatctcagaagcccatgtatctccagcagagcttgcaagagcacccaggagggataaacacatccaagatgcagtgtactctttggaaaaatc
tattggtatttctccaggtccattttctatcatgtagagcagagctaattcagcaggaaggccaccattgcagaacacttgaacccaattccttt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: