XB-CLONE-3171208
Clone Name: IMAGE:7856524
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Gene:
psat1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127350 [+]
GTTTATTTGGAGCTTGGGATGAAGGTTTGGAGGAGAAATCTGTGCAGGTTACGCTGCATTGGTGTTTAGCGTTCTGCCTCTGAGACCTACGCTCTGTTAT
AGCCATGGACATCACTAGGGATGTACTGAATTTCGGTGCTGGACCTGCCAAGCTCCCGCCATCGGTTCTGCTTGAAGCACAGAAAGAACTGATTGACTAC
AGAGGACTTGGCATCAGTGTTCTAGAAATGAGCCATCGATCATCTGATTTTACAAAGATTATGAACACCACAGAAGTTTGCTTGCGTGAATTGTTGGATA
TTCCAGATACCTACAAGGTGCTTTTTCTTCAAGGAGGTGGAAGTGGACAATTTAGTGCAGTTCCCCTTAATCTTATTGGTTTAAAGGATGCCAAATGTGC
GGATTATGTGGTTACTGGAGCTTGGTCTGCAAAGGCAGCGAAAGAGGCAGAGAAATATGGAAAAGTAAACATTGTTCATCCAAAGCTTGGAAGTTACACA
GGGATTCCAGATCCATGCAGCTGGAATCTTAACCCAGAAGCTTCATACGTATATTACTGTGCAAATGAAACTGTTCATGGAGTGGAATTCCAGTTTGTTC
CTGAGGTTAAGGGAACAGTTCTAGTCTGTGACATGTCCTCAAATTTCCTTTCTAAACCAGTTGATGTTTCTAAGTTTGGTGTAATTTTTGCTGGAGCTCA
GAAAAACGTTGGCTGTGCAGGGGTGACAGTAGCCATTGTGCGAGAGGATTTGCTGGGTCACTCTCTAAAAGAATGCCCAACAGTTCTGGATTATAAAATC
CAAGCAGGAAATGGTTCCTTGTACAATACACCTCCATGTTTCAGCATCTATATAATGGGACTGGTGCTCGAGTGGATTAAGAATAATGGAGGTGCTGCAG
CTATGGAGAAATTAAGCATCATTAAATCTAATATGATTTACAGCATTATTGATGAATCCAATGGATTATTTGTATGTCCAGTAGAAGAGAAGAGTAGAAG
CAAAATGAATATTCCTTTCCGTATCGGAAATATCAATGGGGACAGTGATTTGGAAAAACAGTTTCTTGCCAAAGCTGTAGAACGTGGGATGATGTCTCTG
AAAGGCCACAGGTCTGTGGGAGGCATAAGGGCTTCTCTGTACAATGCTGTTACTGTGGAAGATGTGCAGAAGCTTGCAGACTTTATGAAATCCTTTCAGG
AATCACACCAGTAATGGACTCAAGCAGTATTATTTCAAGCTTCACCATCATGGACAGCTGTAATAAAATGGCACTTACTAATATGTTGTTCATAGTTTGT
ACAGTATTGTTTGTAATTTTATCATTCTTTTGTAATGAATCAGGGGTGTCTGTTTGTATTCCCCATCTCTTTGCACAAAAAGGCAGTTAAAAAGCTAAAG
TAACAGTAAAGCTGGAATGCTTTCCTTTTGCTAAAATTGTAGTTTGTGAAATGTGTAAAGCTTAAATAACTGAACAAATGCATCATCCTGATAGTCTGAT
GTAATTTATCTGGTGAAATAAGACTATTGTATCCTGTTTGCTAAAGGGACCAATATGTATGTATTTTCCTTATGGCTAAGGAATTAAGGCAGAAATAACA
TGTATGATAGTTTTTTTTTTTTTCTTACATTCCCTTCAACTGCTTTATAATGTTCTGTTCTAGTGGTGCTACAGAACCAACTGGTTGGTACCTGCTGCTT
TGGCAAAAAGATACAAGACACAATGCAAAGGTTAAAACGTGTTTTTGTGATGCAACTTTTACATTGCCTTGAATAATGTATTTAAAAAA
5' EST: DT400270 [+]ttatttggagcttgggatgaaggtttggaggagaaatctgtgcaggttacgctgcattggtgtttagcgttctgcctctgagacctacgctctgttatag
ccatggacatcactagggatgtactgaatttcggtgctggacctgccaagctcccgccatcggttctgcttgaagcacagaaagaactgattgactacag
aggacttggcatcagtgttctagaaatgagccatcgatcatctgattttacaaagattatgaacaccacagaagtttgcttgcgtgaattgttggatatt
ccagatacctacaaggtgctttttcttcaaggaggtggaagtggacaatttagtgcagttccccttaatcttattggtttaaaggatgccaaatgtgcgg
attatgtggttactggagcttggtctgcaaaggcagcgaaagaggcagagaaatatggaaaagtaaacattgttcatccaaagcttggaagttacacagg
gattccagatccatgcagctggaatcttaacccagaagcttcatacgtatattactgtgcaaatgaaactgttcatggagtggaattccagtttgttcct
gaggttaagggaacagttctagtctgtgacatgtcctcaaatttcctttctaaaccagttgatgtttctaagtttggtgtaatttttgctggagctcaga
aaaacgttggctgtgcaggggtgacagtagccattgtgcgagaggatttgctgggtcactctctaaaagaatgcccaacagttctggattataaaatcca
agcaggaaatggttccttgtacaatacacctccatgtttcagcatctatataatgggactggtgctcgagt
3' EST: DT400269 [+]atacattattcaaggcaatgtaaaagttgcatcacaaaaacacgttttaacctttgcattgtgtcttgtatctttttgccaaagcagcaggtaccaacca
gttggttctgtagcaccactagaacagaacattataaagcagttgaagggaatgtaagaaaaaaaaaaaaactatcatacatgttatttctgccttaatt
ccttagccataaggaaaatacatacatattggtccctttagcaaacaggatacaatagtcttatttcaccagataaattacatcagactatcaggatgat
gcatttgttcagttatttaagctttacacatttcacaaactacaattttagcaaaaggaaagcattccagctttactgttactttagctttttaactgcc
tttttgtgcaaagagatggggaatacaaacagacacccctgattcattacaaaagaatgataaaattacaaacaatactgtacaaactatgaacaacata
ttagtaagtgccattttattacagctgtccatgatggtgaagcttgaaataatactgcttgagtccattactggtgtgattcctgaaaggatttcataaa
gtctgcaagcttctgcacatcttccacagtaacagcattgtacagagaagcccttatgcctcccacagacctgtggcctttcagagacatcatcccacgt
tctacagctttggcaagaaactgtttttccaaatcactgtccccattgatatttccgatacggaaaggaatattcattttgcttctactcttctcttcta
ctggacatacaaataatcccattggattcatcaataatgctgtaaatcatattagatttaatgatgcttaatttctccatagctgcagca
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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