XB-CLONE-3171141
Clone Name: IMAGE:7856578
|
Gene:
npepps
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC124025 [+]
GTTCGCTCCGTGTTCTCCTCACTCGTCCCGTCTTCTCTTCTCTTGCCCTTACCTCTCCTTGTGGCCGGTGTTTCGGTGCCGCTATCCCTCAAATTGCCAT
GCCGGATCGCAGACCTTTCGAGCGGCTGCCGACTGACGTGCGGCCCGTCAACTACGGACTCTGTCTAAAGCCTGACCTCATAGACTTTACCTTCGAGGGG
AAGCTGGAGGCCACTGTTGAGGTGCAAAATGCAACCAACCAGATTGTGATGAATTGTGCAGACATCGATATCATCACTGCTTCTTATGCTCCCGAGGGTG
ACGAAGAAATTCATGCTACTGGGTTTAACTACCAAAATGAAGATGAGAAGGTTACTCTGTCCTTTCCTAGCACCTTGCAGAAAGGTGCAGGGATGCTAAA
GATTGATTTTGTTGGAGAGCTGAATGATAAGATGAAAGGATTCTATCGCAGCAAATATGCGACTGCAACTGGAGAGGTGCGATATGCAGCTGTCACACAG
TTTGAGGCAACAGATGCACGCAGAGCCTTTCCATGCTGGGATGAACCTGCTATAAAGGCCACTTTTGATGTCATCCTAATTGTACCTAAGGACAGAGTGG
CTTTGTCTAATATGAACTTGATTGATAGAAAGCCATACCCAGACGATGAGAACTTAGTAGAGGTGAAATTTGCACGCACCCCTATCATGTCTACATATCT
TGTGGCCTTCGTTGTGGGAGAGTATGATTTTGTAGAAACAAGGTCTTCTGACGGCGTGCTGGTCCGTGTTTATACACCTGTTGGAAAAGCAGAACAGGGG
AAGTTTGCCTTGGAGGTTGCTGCTAAAACCCTGCCTTTTTACAAGGATTACTTCAACGTACCCTACCCCCTCCCCAAGATTGACCTTATCGCCATTGCAG
ACTTTGCAGCTGGAGCCATGGAAAACTGGGGCTTGGTCACCTACAGGGAGACTGCTCTGCTGATTGACCCCAAGAACTCCTGCTCTTCTTCTCGCCAGTG
GGTGGCATTAGTTGTGGGACATGAACTGGCACATCAGTGGTTTGGGAACCTAGTCACCATGGAGTGGTGGACCCACCTCTGGCTGAATGAGGGCTTTGCT
TCTTGGATAGAATACCTTTGCGTAGACCATTGTTTTCCAGAGTATGACATCTGGACACAGTTTGTCTCAGCCGACTATACCCGGGCACAGGAACTAGATG
CACTTGAAAACAGCCATCCCATCGAGGTCAGTGTCGGACACCCATCTGAAGTTGATGAAATCTTTGATGCTATATCCTATAGCAAAGGTGCCTCTGTCAT
CCGCATGCTACACGATTATATTGGGGATGAGGATTTTCGCAAGGGCATGAATCAGTACCTCACTAAATTCCAGGAGAAGAATGCTGCCACAGAGGATCTC
TGGGAATCTTTGGAACAGGCCAGTGGGAAACCCATTGCTGCAGTAATGAACACCTGGACTAAACAGATGGGCTTCCCCCTTATATGTGTGGAATCAGAGC
AGAAAGAAGATTCCGTTGTCCTGAAGCTTTCCCAGAAGAAATTTTGTGCCAGTGGAGCTCCTAACAGCGATGACAGTTACCAGTGGATGGTACCAATTAG
CATCTGCACCAGTGAATCTCCAGCATCTGCTACTGTGAAGATTCTAATGGATAAGCCAGAGATGACTGTGGTGCTGGAAGGTGTTAAGCCACACCAGTGG
GTTAAGTTGAATCCTGGCACAGTGGGCTTCTACCGCACTCAGTATAGTACTACGATGTTGGAGAGCCTATTACCTGGCATCCGAGACCTTTCGTTACAGC
CTGTTGACAGGCTGGGTTTACAGAACGACCTGTTCTCTCTGGCTCGAGCAGGTATGATCAATACAGCTGAGGTCCTGAAAGTTATGGAGGCATTTGTAAA
TGAGCCTAATTACACTGTGTGGAGTGACCTGAGCTGCAACCTGGGGATCCTCTCAACGCTGCTTTCCCACACAGACTTTCATGAGGAAATACAGTGCTTT
GTCAGGGATGTTTTTTCCCCAATTGGGCAACGCCTTGGCTGGGATCCTAAACCTGGAGAAGGGCACTTAGATGCTCTTCTTAGGGGTCTTGTCTTGGGAA
AGTTGGGGAAGGCTGGCCACCAACCGACCCTTGAAGAAGCCAGACGACGGTTTAAGGAACATGTAGACGGACGAAATGTATTGAGTGCAGATCTGCGGAG
CCCGGTTTATGTGACGGTTCTGAAACATGGGGATAATTCAACCTTGGAGACCATGATGAAGCTCCACAAACAAGCTGACATGCAGGAGGAGAAAAACAGG
ATCGAACGAGTTTTGGGAGCCATCGCAGAGCAGGATCTGATCAAGAAGGTGTTGAGCTTCTCTCTGTCGGAAGATGTTCGTCCACAGGACACCGTGTGCG
TCATTGGAGGAGTGGCGGGTGGCAGTAAACTTGGCAGGAAGTGCGCCTGGAACTTTGTGAAGGATAATTGGGAGGAATTGTATAACCGTTACCAGGGTGG
CTTCCTCATCTCTAGGCTTATAAAGCTCTCCTTGGATGGTTTTGCAAGTGACAAAATGGCAGCAGAGATTAAGGCATTTTTTGATGCCCACCCAGCTCCC
TCTGCAGAGCGCACGGTGCAGCAGTGCTGTGAGAATATTCTGTTGAATGCTGGCTGGCTGAAACGTGATGCAGAAGCCATCCACCATTATCTACTGCAGC
GCAAGCCATCTTCCTCCACCGTGTGATTGTGCACACTCATCTCTCCACCTGCTGTTTCACCGCCCCAACAGGCTAGACCCTAGGCTTCCATCACTGGAGG
CTCAGTACCTACCTTTCCAGATACCCTCCCCCAAATTCCTCTCCCCGCCAGGTGAGTGGGTGCTAAAAGTGGCCCCCTCCATTCGGCACCTGCTCAGTCC
CCAGTCAACTCCTTAGGGAATTTATTCAATTGCTCAGCCTTCCTAAAGAGGTAGCATACAGCTGTGTGTGACAACTGTGAATTTACTAGGTGGCCCATAA
ACAAAGGAACTCTGAGCCCATTTTGGAGCAGAGTTCTGTAAGTTAGAGGTACCAGGCACAAGTGCAGAGGATTATTTGCAGGTAAAGGGGCTGTAACCTG
ACAGGTTATGACAGTTATGTGCTTTACCCATGGCCGCGTAGGAGGATTGCTGGCTTTCCCCTTTTCTTTAATAAGGAGATACTCCCCTCCCTCTCACTCC
TTACTGTTGCCACTGTCAAAATCTAATCATATGCCGACTGTTGTGTTTGTCCTGGAGTTTAACCTAACCCTTTACTCTGGGCATAGAGTTTAACTTGAGG
GGCAGTTCAAGTTTGCACGTTTGTGTACTTACACTTAACCCCATTTCCAGCTGCTCTGTACCTTTCAGTGGCCTGGACCGTTATCCCCCAACAGTACAGA
AAATGCTTGCACAGTGGTACTGAAGGGTCTCCTTAATGTAGATTTCTCCTCTTAGCCACACTGAAGAGGGACAAAGTGCAAAGTATCTTGTTCCTAAAGT
CAACTAGTTATACCAGTTCCAGATAAAACCCTAGGTTAGCCAGTTTGCAGGTATTGTGCAGTCTAACGGACTAGCGGTTGGGCAATAAGCCTTGGTTATC
AGACTTGCTTACCTGAGTTCTCTCTTTTGTGCTATTGTTTTAAATGACAAAAACAAAAAAGAATCAAAAAAAA
5' EST: DT400314 [+]tcgctccgtgttctcctcactcgtcccgtcttctcttctcttgcccttacctctccttgtggccggtgtttcggtgccgctatccctcaaattgccatgc
cggatcgcagacctttcgagcggctgccgactgacgtgcggcccgtcaactacggactctgtctaaagcctgacctcatagactttaccttcgaggggaa
gctggaggccactgttgaggtgcaaaatgcaaccaaccagattgtgatgaattgtgcagacatcgatatcatcactgcttcttatgctcccgagggtgac
gaagaaattcatgctactgggtttaactaccaaaatgaagatgagaaggttactctgtcctttcctagcaccttgcagaaaggtgcagggatgctaaaga
ttgattttgttggagagctgaatgataagatgaaaggattctatcgcagcaaatatgcgactgcaactggagaggtgcgatatgcagctgtcacacagtt
tgaggcaacagatgcacgcagagcctttccatgctgggatgaacctgctataaaggccacttttgatgtcatcctaattgtacctaaggacagagtggct
ttgtctaatatgaacttgattgatagaaagccatacccagacgatgagaacttagtagaggtgaaatttgcacgcacccctatcatgtctacatatcttg
tggccttcgttgtgggagagtatgattttgtagaaacaaggtcttctgacggcgtgctggtccgtgtttatacacctgttggaaaagcagaacaggggaa
gtttgccttggaggttgctgctaaaaccctgcctttttacaaggattacttcaacgtaccctaccccctcc
3' EST: DT400313 [+]ttttttttgattcttttttgtttttgtcatttaaaacaatagcacaaaagagagaactcaggtaagcaagtctgataaccaaggcttattgcccaaccgc
tagtccgttagactgcacaatacctgcaaactggctaacctagggttttatctggaactggtataactagttgactttaggaacaagatactttgcactt
tgtccctcttcagtgtggctaagaggagaaatctacattaaggagacccttcagtaccactgtgcaagcattttctgtactgttgggggataacggtcca
ggccactgaaaggtacagagcagctggaaatggggttaagtgtaagtacacaaacgtgcaaacttgaactgcccctcaagttaaactctatgcccagagt
aaagggttaggttaaactccaggacaaacacaacagtcggcatatgattagattttgacagtggcaacagtaaggagtgagagggaggggagtatctcct
tattaaagaaaaggggaaagccagcaatcctcctacgcggccatgggtaaagcacataactgtcataacctgtcaggttacagcccctttacctgcaaat
aatcctctgcacttgtgcctggtacctctaacttacagaactctgctccaaaatgggctcagagttcctttgtttatgggccacctagtaaattcacagt
tgtcacacacagctgtatgctacctctttaggaaggctgagcaattgaataaattccctaaggagttgactggggactg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: