XB-CLONE-3171001
Clone Name: IMAGE:7856638
|
Gene:
dhrs12
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC122052 [+]
GGGATGTCTCTGTACAGAAACACCATCTGGTTCCTGAAGGGGATGAGGGAGTACACCAAAAGTGGGTATGAGACTGCAGCAAAACAATTTTTATCAGAAG
ATTTGGAGTTCGATGTCAGGGGAAGGTCTTATATGATCACTGGAGCAAACAGCGGGATTGGCAAAGCTGCTGCCTTGGTAATTGCAAAGAAAGGTGGAAC
TATCCACTTGGTGTGCAGAAATAAGGAAAGAGCAGAAGAAGCCCAAAGAGAACTCAAAGCAAACAGTGGAAATGAGGACATTTCAGTGCATTTGCTGGAT
ATGTCTGACCCCAAACAAATATGGGAATTTGCTGAGAAGTTCAAGACTGAGCATAAACTGAATGTCTTGATAAACAATGCCGGGTGCATGGTTAATAAGC
GGGAACTGACTGAAGATGGCCTAGAGAAAAATTTTGCCACCAATACTTTGGGTACATACATTCTAACAACTGCACTGCTGCCATCTTTGGAGAAAGAAGA
GGATCCCAGAGTTATAACGGTTTCCTCGGGAGGAATGTTGGTTCAGAAACTGAACGTGTCAGACCTGCAGTTTGAGACAGGCACATTTGATGGCACAATG
GCATATGCACAGAACAAGCGACAGCAAGTAATATTAACAGAGCAGTGGGCTAAAGCCAACCCGAATGTCCATTTCTCTGTCATGCACCCAGGCTGGGCTG
ACACACCAGCTGTGAGATCCTCAATGCCAGATTTCTATGAAAAGATGAAGAACAGGTTACGCACAGAGGAGCAAGGTGCAGACACTGTTGTATGGCTGAC
ACTTTCCCCCTCTGCCAAGAAGCACCCGAGTGGCCTTTTCTTTCAGGATCGGAAACCTGTTTCTACACATTTACCCTTTGCGCTTACACATTCCTCTCCT
GGGGATGAAGAGAAACTTATGGAGAGCTTGAAGGAACTGTCCCAAAAATTTGCACCCGCTTCCTGTAAATTGTAACACTTCCTCAATAACTGTTAACTTA
CCAGGCGGCTTTAATAGTGCAGATTGCGAATGTACATAAACTCATTTGGTAGGTGAATAACAATACAAGAAACTCAAAGCAAATTTTGTACTTTGCAGTT
GACACTGGATTAAGCCATACACTTTTTAAGGCTTCAGTAAGCGGCTGCAGAACTCCATTTTGATGCAGACTATATTTCTTTTATTTCTACCAAACAGCTT
TAGGCTTATGGTACACAGGGCAAATTGTCCACTGCTGCTCACTTGAGTGTTTATGTATGGAGGTCCATCTGTCAACACTTTGTTTGCAACAGACAGAGCA
CTTTGCTTTTGATAACCTCAAGACTGATAACAGATGGACAGGGGCAGCTTGCCTACTTCAGCAGAAAAATGCAGCAGCAATTGGTGGGCAAAATGTCCCT
GATATTGACATGAACAGTGACCTCTATCTAGCTATCACTCCTCATGTGCATCAGCCAGCGTGTGTGCCGTTGGCAGAGGCATCTAGACCACTGGTCTGTG
TGGGATGATGGTAGCAGTCAAATTGCTCCTTGTGCCTGCGGCTCTGAGCTTTGCATCTCCTTGAGTTTACTTTAAATAGGCCACAAGGTAGGCGAGGGGC
TGATCAACAAAGCTTCCCTTCTCACTCAGTTATAAGATACATTGCCAGGCATTCAGGTAACCCTGTAATTATCAGCTGCTTCAGGGTTCTGTATGGGGGG
CAGAACCCAGGTTACTAAAGTCAGCTGCAGTATCTTGACCACAGAACTAATAAGGACGCTTCTGCCCTGCTAATGAACCCAAGGTACTGTCTGTCTGAGA
CCAGGAAGTTGGTTTGTACTACAAGCTGACAGAACAAATGACAAAATTGCAGTGTATGTTCATACAAAGTATATGGCTGCTATTATACATTCTAACTATA
TATAATTATATTCAGGATCTCATTTTTATCACTTTATTTTCTTTTGTATATCACCTCAGAAAAAAAAAAAAAAAAA
5' EST: DT400407 [+]ggatgtctctgtacagaaacaccatctggttcctgaaggggatgagggagtacaccaaaagtgggtatgagactgcagcaaaacaatttttatcagaaga
tttggagttcgatgtcaggggaaggtcttatatgatcactggagcaaacagcgggattggcaaagctgctgccttggtaattgcaaagaaaggtggaact
atccacttggtgtgcagaaataaggaaagagcagaagaagcccaaagagaactcaaagcaaacagtggaaatgaggacatttcagtgcatttgctggata
tgtctgaccccaaacaaatatgggaatttgctgagaagttcaagactgagcataaactgaatgtcttgataaacaatgccgggtgcatggttaataagcg
ggaactgactgaagatggcctagagaaaaattttgccaccaatactttgggtacatacattctaacaactgcactgctgccatctttggagaaagaagag
gatcccagagttataacggtttcctcgggaggaatgttggttcagaaactgaacgtgtcagacctgcagtttgagacaggcacatttgatggcacaatgg
catatgcacagaacaagcgacagcaagtaatattaacagagcagtgggctaaagccaacccgaatgtccatttctctgtcatgcacccaggctgggctga
cacaccagctgtgagatcctcaatgccagatttctatganaagatgaagaacaggttacgcacagaggagcaaggtgcagacactgttgtatggctgaca
ctttccccctctg
3' EST: DT400406 [+]ctgaggtgatatacaaaagaaaataaagtgataaaaatgagatcctgaatataattatatatagttagaatgtataatagcagccatatactttgtatga
acatacactgcaattttgtcatttgttctgtcagcttgtagtacaaaccaacttcctggtctcagacagacagtaccttgggttcattagcagggcagaa
gcgtccttattagttctgtggtcaagatactgcagctgactttagtaacctgggttctgccccccatacagaaccctgaagcagctgataattacagggt
tacctgaatgcctggcaatgtatcttataactgagtgagaagggaagctttgttgatcagcccctcgcctaccttgtggcctatttaaagtaaactcaag
gagatgcaaagctcagagccgcaggcacaaggagcaatttgactgctaccatcatcccacacagaccagtggtctagatgcctctgccaacggcacacac
gctggctgatgcacatgaggagtgatagctagatagaggtcactgttcatgtcaatatcagggacattttgcccaccaattgctgctgcatttttctgct
gaagtaggcaagctgcccctgtccatctgttatcagtcttgaggttatcaaaagcaaagtgctctgtctgttgcaaacaaagtgttgacagatggacctc
catacataaacactcaagtgagcagcagtggacaatttgccctgtgtaccataagcctaaagctgtttggtagaaataaaagaaatatagtctgcatcaa
aatggagttctgcagccgcttactgaagccttaaaaagtgtatggcttaatcccagtgtcactgc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: