XB-CLONE-3169773
Clone Name: IMAGE:7857276
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Gene:
kdf1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122053 [+]
GTTTAAGCAGCGATCCGGGACTGCGATCCGGGACTACAGTCCCGAAAAGCGGGACTGTGGGCGGTGTGCGATAGGTGATTAGAGTATATTGATTTGAAGT
GTTTGCATGCGAAGCAATGCCCCGACCCAACCCCGTGGCTGCCCCACGTGGGCGGACCTCACCCCTTAATAACCATGCTTCAGTCCATCGTACCTCACCA
ACACCGCCGGCAAACAAGTCTACCTCCAGGCGAAGGAGCCCCCCTGAAGACATAACATTCATTTCAGGCTCTGCTGAAACACCTCCTGAGGAGCCTCCTT
GCTGCCCTTCACTTTGCAGAGCATGGGCTGCCTATAAAGCAGTTTTGTGTTATGTAGTGTCCTGCAGTCGCTGCCTTCAAAAAGACCCTGATATATATAT
TGCATACCCCTTGGAAGTCAGAGATATCGGTCCCCACAATGGCCATCCTGCTGGCAGCCCTCATCCTCCACGTGCTATTGAAAGGGGAAAGAAAGCTGGG
CTTGGTAACAGCTTTAGTTATCCAGATGTTAAATTATTGGGGGTTCCTGTTTACAACAAACAGCCATGTCCAGTCAATACAGACACAGAGGCTACAAAAG
ACTACACAGCTCCTGTTTCACAACTTGCTCCTCGCAGCTCTGCCGGTGACTACTATTCATTGCAGGAGTCTGATCCTGAACTTTCGAGGTGGAGCAGCTC
CATGTCCAGTGCAGAAATAGATGTGTTGATATGGCAAAAGCTAACTGAACTGTTCAGTCTCCATCAAATTGATGAACTAGCAAGGTGTACTTCAGAAACC
GTTTTCTTGGAGAAGAGCAGCATGATAACAGATTTGATTAACAGTCTGACGCAGGATTACCAGTTGGAAGAGCAGGATGCAGAATGCCGGCTGGTGCGGG
GCATTATCCGTATCAGCACTCGTAAAGTTCGTCCACGTGGAGGAGAAAAGGGATATGGAGGGCAGCATCATAACAGCCGGAATAATGGCAAGGCACCTGA
TAGTGGCAATGACACCATGCATGAGTCTGGACTGACAAGCCAAGATGATCTGGATGTACAGATATCAGAAGAGACAACATCAGACCTCAAAGCAAGAAAC
ATGAGGAGATACAGCACACCAGGGACACCTTTGTTGAAAGATGATTCTTTACAGGACACAGAGACAGATTCTTCAGGAACACCTCTGCTAAAAGTATATT
GTTAACTAAAGACTATAGAGAGCAGAATCTTCTAAATGAAGGTGGTGATCTGAGCACAGAGCCACTCTGATGTACATAATGGTAAAGTGCAATATCAAAC
ACAGGATTAAAGTTCCTTTCAAAAGGTGAAGAACGCAGCCTTTTATCAATTTCTACTGTTATCCTGCTGCCCGGATTCTATTTTTCTGTTAGTGCAGATT
AGGGGGAAAGCTTTCTTTTGGAATTTTTTGTAAACAGGACTGAACTACATAATGTTTGTTGTATGAACATTTAAATAGGATTTTTAAAACCATGGTCACG
TGGATTTTTTTTTTCTTTCTTATAAATATATAGACACTTTATAGAACTTGCAGATGGATCTAGTTTCTTTTCATATCACTTTCACCTGTTGGCAGAATAT
TTTGACTGTACACTTCAAAAGTGGGAACTTACAAATGAATTTCAACTGTACATATATGTGTGTATGTGAACAAAGGACAAATAGCCTCATTGGTAGGTGA
TGCTGCAAGAAATGAAGCATGCCTTTAAAGGGTCCTACGTGGCCTGCCTGTGGAAAGGAGGAAACAGACTGCAACTCTTACACTGCTCATACTGTAAATC
CCACCGTCTAATGTAGGTTTTGGTATTTCACAGAGAAAATGGTACGTGGAATATGTTAAAGTTTTAATACAATGCATTCTGAATACATTACAGTTGTTGG
AGAAGCAGAATCATTAAATGGATACTTGTAAAAAA
5' EST: DT401218 [+]ttagcagcgatccgggactgcgatccgggactacagtcccgaaaagcgggactgtgggcggtgtgcgataggtgattagagtatattgatttgaagtgtt
tgcatgcgaagcaatgccccgacccaaccccgtggctgccccacgtgggcggacctcaccccttaataaccatgcttcagtccatcgtacctcaccaaca
ccgccggcaaacaagtctacctccaggcgaaggagcccccctgaagacataacattcatttcaggctctgctgaaacacctcctgaggagcctccttgct
gcccttcactttgcagagcatgggctgcctataaagcagttttgtgttatgtagtgtcctgcagtcgctgccttcaaaaagaccctgatatatatattgc
ataccccttggaagtcagagatatcggtccccacaatggccatcctgctggcagccctcatcctccacgtgctattgaaaggggaaagaaagctgggctt
ggtaacagctttagttatccagatgttaaattattgggggttcctgtttacaacaaacagccatgtccagtcaatacagacacagaggctacaaaagact
acacagctcctgtttcacaacttgctcctcgcagctctgccggtgactactattcattgcaggagtctgatcctgaactttcgaggtggagcagctccat
gtccagtgcagaaatagatgtgttgatatggcanaagctaactgaactgttcagtctccatcaaattgatgaactagcaaggtgtacttcagaaaccgtt
ttcttgg
3' EST: DT401217 [+]ttttttacagtatccatttaatgattctgcttctccaacaactgtaatgtattcagaatgcattgtattaaaactttaacatattccacgtaccattttc
tctgtgaaataccaaaacctacattagacggtgggatttacagtatgagcagtgtaagagttgcagtctgtttcctcctttccacaggcaggccacgtag
gaccctttaaaggcatgcttcatttcttgcagcatcacctaccaatgaggctatttgtcctttgttcacatacacacatatatgtacagttgaaattcat
ttgtaagttcccacttttgaagtgtacagtcaaaatattctgccaacaggtgaaagtgatatgaaaagaaactagatccatctgcaagttctataaagtg
tctatatatttataagaaagaaaaaaaaaatccacgtgaccatggttttaaaaatcctatttaaatgttcatacaacaaacattatgtagttcagtcctg
tttacaaaaaattccaaaagaaagctttccccctaatctgcactaacagaaaaatagaatccgggcagcaggataacagtagaaattgataaaaggctgc
gttcttcaccttttgaaaggaactttaatcctgtgtttgatattgcactttaccattatgtacatcagagtggctctgtgctcagatcaccaccttcatt
tagaagattctgctctctatagtctttagttaacaatatacttttagcagaggtgttcctgaagaatctgtctctgtgtcctgtaaagaatcatctttca
acaaaggtgtccctggtgtgctgtatctcctcatgtt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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