XB-CLONE-3165878
Clone Name: IMAGE:7858694
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Gene:
slc66a2.2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC124031 [+]
CTTTCAACACAAGATTTTAATGGTCACACTACTTATGCTGGTTATATTGTAAATGGTCAAGACTGAGTCATTTTTCTATTTCCCCTCATGCCATTCTCAG
GTTGGGAAAATAGATTATGTCAATGAGATGAGCAAAAAGGGAATTGTCTAATTATGGAAAAACTTACAATACCAGTAATGATCTGCTTTTTTTTTTTAAT
CCAGTCATGTTTCATTTGACATCTATTTAGCGTTAAACCAAATAAATGTCTTCTATTTTTTTCCTGTATTAAACCCCATGTCAGTACAGAGACAGATCAC
TAATTGCAAGTGTAGATATTGATAAATAATAATGGATTTTAAACACAACACAGTCTTTTTAAATTAGACTTTGCAAATTAAAGTAATTATGATAAATTGT
GCCCTATAAACAATAAGCTGACAGCACCTGCAATTACATTTCCAGGGCAAAGAAGTTGAAAAGGCTTTAGAATATTGATTCAAAATGGAACAGATAGAGG
TTTCTGATGATTCCTGGCCGTGGTTTTCATGGATTTCTTCATCTCTCATCGTCTTCGGTGGTGTGGTCCCATATATCCCACAGTATCAAGAGATTAAAAG
GACTTCCAACCCTGATGGATTTTCTACTTGGGTATGTTTGGTCTTGCTGGTGGCTAATATCCTCAGGATATTTTTTTGGTTTGGAAAGTTTTTTGAATTC
CCCCTCCTATTGCAGAGTATCATGATGATCCTTACTATGTTAACCATGTTGCACTTGTGTTGCTCACTAAAGACTATGAACAGAGTTTCCACTAAGAAGC
ATTGCTTTACTGATTTTGAAGCTGCATACTTTTGGAAGTGGAGTCGATTTGAAGATTATCTCCAATTTTGCCTGTGCCTGACCATCATTGCTGCCCTGGT
GACCTTTGTTTTTCTGGATGTCTTGGTGTTTGTGGAAGGAATGGGGCTACTGGCTCTACTCACTGAGGCTATGCTGGGGATGCCCCAACTTCTGAGGAAT
TATAAGAACAGATCCACACACGGAATGAGTGTGACGATGGTCTTGCTGTGGACAACGGGGGACTGTTTTAAAACTGCCTATTTTATTATTAAAGAAACTC
CTGCTCAGTTCTGGCTTTGTGGAACAATACAAATATGTATAGACATTGCTATACTTCTGCAGGTGCACTACTATAATAAGGAGCATTCCAAGCATGGATA
ATGGAGATGACCTTCACAAAAACCTGGTGCCATGGTTACTGAATTACCATTTTGGATATACAAAGTATATGTTGTTCATTGTGGGGTATTTTATTGCTTA
GGTTTTTTGAATCGGTCAATGTACCAAGCTGCGTGTTACTAAAAATTTTTAAGACTCCTTTTGATCCTTTTAAATAATATATAGATGTGCAGGCACAGTG
GGTCAAGGAAATGTGGAGGCTACATACTGATTCCAAGTTTGCACTGGTGTCCTTTGCCTTAAATATGTATTTGTAAATATAAGAGGGGATACTTTTTACC
CATACGGGGATCTGAGAATAAAGCCCAGACTGCTCCCCACAGACACATTATAGGTCTATGGCAGCTTGCAGCGATCCCTCTGGCAATTACTATAATATCC
AGATTGCTAGTCTGGGCCTGCTTGTATCTCCTACATGAAAAATTGGGTAGAGGTGATTCTCCTGATGTTACATAAAATATATATACTAAATAGAGATTCT
CTTAATATAAATATATTTATATATACCGGTATATATATATTTTTTAAATATTTATAAACATTGTGGTATTGCAAGTAGCTGCAATGCAGTGCTAAGTGGA
GAGCTGACATATTTTAATAAAGAAATGTAATTAACAAATTATCGTGAAAAATAGCCAAATACATTTTTAGGTGCTTTAACTGTAGAACCTATATCGCTAC
TATGGAATTTCCTTGTCCAGGTTTTTTTTACTCAAGGTAGCAAGACAGAGTTCTGTTCATTAAGCCCTTGGTAAATTAAGTCTCAGTAGTGAAGATATTG
TTGGTACTGAAGGAGAACAGCATATGAGAACACATATGTGAGTAGTACACAGTAATATATATATATTATATATATATACAGTACATTGTTATTTTGTATT
TCATTATTTCTTTAGACTTTACTTAGGGTATGTTTTTAGCTGTGCGCTCTGACTCATTCTGCTTCACTCCCTCCTTCCAGTTCTTCCCCAGATGGCAGCT
TGTGGGATAGAAGCGATTTGCGCCAAAAACCAAAGAAAACATCTTAGGAGACAAATGTCACAGCAGAAAATGGCAAAGTGAATAGCAATCAGTTCAACTG
AGTTGCAGGGTTCGGGGTTAATTAGAGCCTATTGGCCAAATATATACAGAAACTGTGTTTTATTCTAGGGCAGTGCAATAGTTGACACTAGCGGCAAAAT
GTATTAAAAAATAAATTTGTTATTTCTATAAAACCTTGACCTGGGACAACAGAATAGAATGAAAATAAACCGAAGGCATACATTTAAAAAAAAAAAAAAA
AAA
5' EST: DT403778 [+]ctttcacacaagattttaatggtcacactacttatgctggttatattgtaaatggtcaagactgagtcatttttctatttcccctcatgccattctcagg
ttgggaaaatagattatgtcaatgagatgagcaaaaagggaattgtctaattatggaaaaacttacaataccagtaatgatctgctttttttttttaatc
cagtcatgtttcatttgacatctatttagcgttaaaccaaataaatgtcttctatttttttcctgtattaaaccccatgtcagtacagagacagatcact
aattgcaagtgtagatattgataaataataatggattttaaacacaacacagtctttttaaattagactttgcaaattaaagtaattatgataaattgtg
ccctataaacaataagctgacagcacctgcaattacatttccagggcaaagaagttgaaaaggctttagaatattgattcaaaatggaacagatagaggt
ttctgatgattcctggccgtggttttcatggatttcttcatctctcatcgtcttcggtggtgtggtcccatatatcccacagtatcaagagattaaaagg
acttccaaccctgatggattttctacttgggtatgtttggtcttgctggtggctaatatcctcaggatatttttttggtttggaaagttttttgaattcc
ccctcctattgcagagtatcatgatgatccttactatgttaaccatgttgcacttgtgttgctcactaaagactatgaacagagtttccactaagaagca
ttgctttactgattttgaagctgcatactt
3' EST: DT403777 [+]aaatgtatgccttcggtttattttcattctattctgttgtcccaggtcaaggttttatagaaataacaaatttattttttaatacattttgccgctagtg
tcaactattgcactgccctagaataaaacacagtttctgtatatatttggccaataggctctaattaaccccgaaccctgcaactcagttgaactgattg
ctattcactttgccattttctgctgtgacatttgtctcctaagatgttttctttggtttttggcgcaaatcgcttctatcccacaagctgccatctgggg
aagaactggaaggagggagtgaagcagaatgagtcagagcgcacagctaaaaacataccctaagtaaagtctaaagaaataatgaaatacaaaataacaa
tgtactgtatatatatataatatatatatattactgtgtactactcacatatgtgttctcatatgctgttctccttcagtaccaacaatatcttcactac
tgagacttaatttaccaagggcttaatgaacagaactctgtcttgctaccttgagtaaaaaaaacctggacaaggaaattccatagtagcgatataggtt
ctacagttaaagcacctaaaaatgtatttggctatttttcacgataatttgttaattacatttctttattaaaatatgtcagctctccacttagcactgc
attgcagctacttgcaataccacaatgtttataaatatttaaaaaatatatatataccggtatatataaatatatttatattaagagaatctctatttag
tatatatattttatgtaacatcagga
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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