XB-CLONE-3163310
Clone Name: IMAGE:7859755
|
Gene:
tm7sf2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC155427 [+]
TAAAGATGGCAGACAAGATTAAAACATCACGTACTAGTGAACTGGAGTTCGGTGGACCACTGGGAGCCCTTCTCTTACTCTTTGTTATGCCTGGAACTGT
CTTGTATCTTCTGGTGACGTGTAGCACAGAAGATGCCAGTGTACTGCGTGTACCAGGCCCTTGGCCCGCTTTGGACACTTTGTGGGACCCTTTTGCAGTG
ATCTTGCTGCTTTCCTGGGTATCTCTGCAGGCTGTGCTGTATATGCTGCCTATTGGAAAGGTAACAGAAGGAATTACTTTAAGAGACAACACCCGTCTGA
AGTACAGAATCAATGCATTACAAGCCATGATGGTGACTGCGCTCATAGCTGCAGGAGCAGTGATGTTTGGGTTCCCGCTCTCCTATGTTTATGACCATTT
CCTGCAGATGGCAGTGGGAGCAGCATTTATAGCTCTAATTCTCAGCATTTTTCTTTATGCCAAGTCCCTTGCAGCCCCTCAATCAGCACTGGCACCAGGC
GGGAATTCAGGTAACCCACTGTATGATTTCTTCATTGGACATGAATTAAACCCCAGGATTGGCTCCTTTGATTTGAAATATTTCTGTGAGCTTCGCCCTG
GACTCATTGGCTGGTGCCTAATAAACCTTTGCTTCCTGATGAAGGACATTGAGCTTCGTGGCTCCCCATCTCTCTCTATGATCTTGGTGTTTGGATTCCA
GGCTTTGTATGTTGTAGATGCCATGAAGCATGAGGAAGCCATCCTCACTACAATGGACATTGTCCACGATGGATTTGGTTTCATGTTGGCTTTTGGTGAC
CTGTGCTGGGTTCCTTTCACCTACTCTCTGCAAGGATATTTCCTAGTCAGGCACCCTCAGGAACTGAGCACTTATGCTGCTGTTGGCATCATCCTACTAA
ACGCTGTGGGATATATCATTTTCCGAGGCTCTAACTCACAGAAGAATGCCTTCCGAAGAAATCCTGCCGACCCCAAAGTAGTTGGGCTTGAAACTATCCC
TACAGCAACCGGAAAACGTCTTCTTGTGTCTGGTTGGTGGGGCCTTGTTCGTCATCCAAATTACTTAGGAGATTTAATTATGGCTCTGGCATGGTCTCTA
CCCTGTGGACTCTCAAACATCCTGCCATACTTCTATGTCATCTATTTCACTGTCCTTCTGGTGCACCGGGAGGCGAGGGATGAACACCAGTGTATGAAGA
AGTATGGAGTCGCCTGGCAAGAATATTGCAGAAGGGTTCCCTACAGAATTTTTCCTTATATTTACTGATCTACTCTGAACTCTCCGCTATACCTCTGTCC
CATTTGGGCCTGGTTCCACAAACAAATGTTTTCTGCTGAAATGTTCTTGTAAGCTGCGTTCTGTTGCTATACACTGCTTTCATTTCTGCCAGTTAATACA
GACAATATAAAACAGAGGACGTAATGAACCCCCAGGTAACATACAGCTTAATCTAATGAAATCATTGCTATAATGACCAATACTAATTAGTGTCAGGTCA
CATAGTAAAAAAAACATGTATTCAGGTACAGGGTTATTCTACATGTCCATGTGCTACTTTGAAAGGAAAAACCTGCATCTTATAAGAGAAAAGCCTGATT
AAAAACTGGTACCTTAATTGGCAGCATGCTTGTGCTACGCACGAATGAAGATGCAGAAGTTATCTTCCCATTCAACTTCATGTACAATTTCATACATATA
GGATTAGATATTTTACATTGATGCACTGACTTTTTTTTTTTAAACAAAATAATTTGCCCATGTAAATTACTTTTAAGCTGTCTATTCTGGAGTGGATAAA
GCTGGGGCTGTCCATATTCTTAATTTATCTGACCCACCTCTTGCAGTCATTCAGCACCTATAACTCGCTAATGATCTACATAGTAAGTGGGGTTGATAAA
AGACACATCCATCCAGTTAAACCTTTCATGCATATAAAACCTGTCTAACTACTAACTGATCCAAAGGAATGCAAAAAAAAACCCCTTATAAAGCCGCTTT
AATTTACCTGAGGGGAAAAAATCCTTCCTGACTCCAGGTTGGCAATTGGACCAGTTCCTGGATCAGCTTGTAAACATATGCCCATATGGCTATTCAGATT
TGCAGTTATAGCTTTCCTACAGCATACTACACAGGGGCAACTGGTATAAATTTACATATATCCAGTTTGTAGTCTATGGAGGTGTCAACAAAACCTGACT
AAATTTCTGCCAAAAAACAAAACCAGTTCTGTCATTTACTGTGTTAACCCCTTTTCCACGCCATTGGGCTATAATCAGTGCAATATAGTGCCTTATTTTA
ATCAAAAAGTATAAATCAAAACAATTTAATTTCTCATTTGTTCAGAAACATTTGCCTTCCACACTGTTTGGGTTTTATTATCTTTATATGGCATATGCCT
GAAGCCTCCCTATTTTTTAGCAGAAATGAAATCATGATGAAGTGCATTACCTATATATTTCTCACATCTGTTCAATAAAATGCTCTGCCCCTAGTGTCCT
ATACAGCGTCCTAGTCACAAATTATGAAGGAATCAGTGATGGCAGTGTTTTGCTTAACATTGAGAAGTATAACAAGTACCCTACTGGCATTTTCCCCCTG
ACCTTGCAGTTTACAGATCATTCTCTTACAAGTCTGTTTCTTAGATTAGGAACACCTAGGACCTTGGTGCTGATGGGAAAAAAGTGTCATTTCCATTGTA
TACATAAAGTAATAGGACAGGCATGAAAATTAATGTTAAAGGAAACTGAATGGGTTTTGATGCAATTTTATATAATGTGTGTATGGGAAAAGGGAGTGGT
ATTGTGTGCATCAATATACAATCTTAATTTAAATTTTCTATGAAAAAAAGAATGTTTGACCTTTTTGTGACAAATTAGAATAAAGAGGATTTTAGAATGA
AAAAAAAAAAAAAAAAA
5' EST: DT405441 [+]taaagatggcagacaagattaaaacatcacgtactagtgaactggagttcggtggaccactgggagcccttctcttactctttgttatgcctggaactgt
cttgtatcttctggtgacgtgtagcacagaagatgccagtgtactgcgtgtaccaggcccttggcccgctttggacactttgtgggacccttttgcagtg
atcttgctgctttcctgggtatctctgcaggctgtgctgtatatgctgcctattggaaaggtaacagaaggaattactttaagagacaacacccgtctga
agtacagaatcaatgcattacaagccatgatggtgactgcgctcatagctgcaggagcagtgatgtttgggttcccgctctcctatgtttatgaccattt
cctgcagatggcagtgggagcagcatttatagctctaattctcagcatttttctttatgccaagtcccttgcagcccctcaatcagcactggcaccaggc
gggaattcaggtaacccactgtatgatttcttcattggacatgaattaaaccccaggattggctcctttgatttgaaatatttctgtgagcttcgccctg
gactcattggctggtgcctaataaacctttgcttcctgatgaaggacattgagcttcgtggctccccatctctctctatgatcttggtgtttggattcca
ggctttgtatgttgtagatgccatgaagcatgaggaagccatcctcactacaatggacattgtccacgatggatttggtttcatgttggcttttggtgac
ctgtgctgggttcctttcacctactct
3' EST: DT405440 [+]cattctaaaatcctctttattctaatttgtcacaaaaaggtcaaacattcttttttttcatagaaaatttaaattaagattgtatattgatgcacacaat
accactcccttttcccatacacacattatataaaattgcatcaaaacccattcagtttcctttaacattaattttcatgcctgtcctattactttatgta
tacaatggaaatgacacttttttcccatcagcaccaaggtcctaggtgttcctaatctaagaaacagacttgtaagagaatgatctgtaaactgcaaggt
cagggggaaaatgccagtagggtacttgttatacttctcaatgttaagcaaaacactgccatcactgattccttcataatttgtgactaggacgctgtat
aggacactaggggcagagcattttattgaacagatgtgagaaatatataggtaatgcacttcatcatgatttcatttctgctaaaaaatagggaggcttc
aggcatatgccatataaagataataaaacccaaacagtgtggaaggcaaatgtttctgaacaaatgagaaattaaattgttttgatttatactttttgat
taaaataaggcactatattgcactgattatagcccaatggcgtggaaaaggggttaacacagtaaatgacagaactggttttgttttttggcagaaattt
agtcaggttttgttgacacctccatagactacaaactggatatatgtaaatttataccagttgcccctgtgtagtatgctgtaggaaagctataactgca
aatctgaatagccatat
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: