XB-CLONE-3154405
Clone Name: IMAGE:7862888
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Gene:
kifc3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127342 [+]
CCCAGGAAGAGATGAGGGCAGTTCACCAAAGTCTGGATGGTGTGCGGAAAAATCTTCTTACCTTGCAGCCAGCACTTCGAACTCTGACCCATGATTACAA
TGCGTTAAAGCGCCAGGTGCGGGATTTCCCTGCTCTACTAAACGAGGCAATAGGAGAGGCCCGGATAGAGTTCTCACAGGCTGTTAAACATGTCAGTGAT
ACAAATGCTGAACTTCTACACAAATACAGGAAAGAACTGCATCTGCGTAAAGAGTGCCACAACCAGTTGGTGCGGCTTCGAGGCAATATACGTGTACTTA
CACGTGTTCGGCCAATAACAACTGAGGATGGAGTTGGCCCTGGGGCTGAGAACGTTGTAAACTTCGACCCAGATGATGATGGTGTTTTATATGTAGCTCA
AAAGGGAAAAGAGATGAGCTTTGAACTTGACAAAGTCTTTAAACCATCTGCAACTCAGGAAGAGGTATTCAGAGAAGTTTCTCCTCTCATTACCTCCTGT
CTGGATGGTTACAGTGTTTGTATTCTGGCATATGGACAGACAGGATCAGGAAAAACCTACAGCATGGAGGGGATTCCCTCTGATCCTGGTATAAATCAAC
GAGCACTGCACCTTCTCCTGTCTGAGGTGAAGGAAAGATCGAACTCCTGGGAGCATGAACTTAGTGTCAGCATGGTTGAGATATACAATGAGACACTGCG
AGACTTGCTGGGGTCAGACCCTTCAAACTCTCTTGAGATAAAGATCTTACCAGGCAGTGTGGGAGAGCTCTATGTTCCTAACCTGACACAAAGGCAAGTG
CAGAGCATGGAAGATATCAATAAGATTTTGGAGTTGGGACACAAACAGCGAGCCACCGAACACACCAATCTGAACACCCACAGCTCCCGCTCCCATGCTC
TGCTTATCCTGACTGCTAAGGGGAGGGAGACGAGCACCGGTATCTGTTCAACAGGCAAACTTTACTTAGTAGACCTTGCAGGATCGGAGCGGGTGTCCAG
ATCAGGCGCAGCTGGAGAACGCCTGCGGGAAGCTCAATGCATTAACCGCTCACTGTCTGCCTTGGGAGATGTGTTTTCAGCTCTCCGCTCACAGCAGGGA
CATATTCCCTATCGCAACTCTAAGCTTACCTATCTTCTACAAGAACCACTTAGTCGGGATGGGAAGGCATTGCTTCTCCTTCAGGTCTCCCCTGCAGAGA
AGAATATAAATGAGTCCCTTTGTTCTTTAAGATTTGGGGACAGAGTGCGAGCAGTTGAGCTGGGAGCCCCAACAAGAAAAATTGAGCAGCTACCAGTGAA
TGCCGAACTGGCAGAGAGTGAAAGCCCCGTATTACGTAGCACTAAAGGCACCTCCATGCGGAAGAAACCTCAGGGCTCAGTTCGACAGAGGACAAGCCCC
CCTTGACCAAATTCATGTCTGCACTGCCATATCAATGTTTCGGCACCATAAGATGCTTTCTCGATGTTTACAAAACTGTTGGTGCATGAGGGATTATCAT
TGGCTGGGACTTCTGCCTTTGTACTCCTGCATGGGATTATGGGAAATCACTTGAACAAAATGCTGTGACTTAATGGAGACCATAGACAGGACCACAAACT
GCACTTTACTCACCACAGATGGCATCACAGATTTTATATTGAATTTCTAAGCACAAATATTGCTGCCTAACTGTATATGTTTGTTAATATATTTGTACAT
TTATATGCACCATTTATTACAATATATTTTTTTTGTCTATATTAACATACCTGTTTCAGAGTAAGAGATAGGGAAATTATATGTCTATACACACTGTATA
TGGCTCTTATATGAAAGGGCAGGTTACAAGCTCAGAAATTGTATATGCATAAAAACTTAGATGAAAAAAAAAAAAAAAAAA
5' EST: DT411279 [+]cccaggaagagatgagggcagttcaccaaagtctggatggtgtgcggaaaaatcttcttaccttgcagccagcacttcgaactctgacccatgattacaa
tgcgttaaagcgccaggtgcgggatttccctgctctactaaacgaggcaataggagaggcccggatagagttctcacaggctgttaaacatgtcagtgat
acaaatgctgaacttctacacaaatacaggaaagaactgcatctgcgtaaagagtgccacaaccagttggtgcggcttcgaggcaatatacgtgtactta
cacgtgttcggccaataacaactgaggatggagttggccctggggctgagaacgttgtaaacttcgacccagatgatgatggtgttttatatgtagctca
aaagggaaaagagatgagctttgaacttgacaaagtctttaaaccatctgcaactcaggaagaggtattcagagaagtttctcctctcattacctcctgt
ctggatggttacagtgtttgtattctggcatatggacagacaggatcaggaaaaacctacagcatggaggggattccctctgatcctggtataaatcaac
gagcactgcaccttctcctgtctgaggtgaaggaaagatcgaactcctgggagcatgaacttagtgtcagcatggttgagatatacaatgagacactgcg
agacttgctggggtcagacccttcaaactctcttgagataaagatcttaccaggcagtgtgggagagctctatgttcctaacctgacacaaaggcaagtg
cagagcatggaagatatcaataagattttggagttgggacacaaacagcgagc
3' EST: DT411278 [+]catctaagtttttatgcatatacaatttctgagcttgtaacctgccctttcatataagagccatatacagtgtgtatagacatataatttccctatctct
tactctgaaacaggtatgttaatatagacaaaaaaaatatattgtaataaatggtgcatataaatgtacaaatatattaacaaacatatacagttaggca
gcaatatttgtgcttagaaattcaatataaaatctgtgatgccatctgtggtgagtaaagtgcagtttgtggtcctgtctatggtctccattaagtcaca
gcattttgttcaagtgatttcccataatcccatgcaggagtacaaaggcagaagtcccagccaatgataatccctcatgcaccaacagttttgtaaacat
cgagaaagcatcttatggtgccgaaacattgatatggcagtgcagacatgaatttggtcaaggggggcttgtcctctgtcgaactgagccctgaggtttc
ttccgcatggaggtgcctttagtgctacgtaatacggggctttcactctctgccagttcggcattcactggtagctgctcaatttttcttgttggggctc
ccagctcaactgctcgcactctgtccccaaatcttaaagaacaaagggactcatttatattcttctctgcaggggagacctgaaggagaagcaatgcctt
cccatcccgactaagtggttcttgtagaagataggtaagcttagagttgcgatagggaatatgtccctgctgtgagcggagagctgaaaacacatctccc
aaggcagacagtgagcggtttatgcattgagcttcccgcaggcgttctccagctgcgcctgatctggacacccgct
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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