XB-CLONE-3151781
Clone Name: IMAGE:7864093
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Gene:
acsf3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166077 [+]
gttcttcactgtgttttgcgttatcctttttgaccctcagtgcttgccgtagttctccacattagtcaggaaggacagaggaaatgttgctgagaagagt
gctgggctagttgaacgagaaagccagtgacaaagccagtcagcagctaccagaccagactgacgagtgaaagctaatatatctcaagtcttttattgga
ctgactttagaaccagcagtgcaaagaacagaactgacagcagcaggaggccactgatcagcaggcagattgacagccctacagcaggattaaaccccat
ggcatgactcaccgatgacatcagctaactgtactttaggattcataaatgaaattatttgcttttcgcagcctgaggattatagaggcatcctagactg
agccatggcaggagggagctgccattttgtttcacagatgcgggttgcatcctttttggggagatttctttatacttggtcatgtgtccgtggggtgcag
gtgagaacaaggaccccactgcattcttgctactttcatgtttcaaaatctagcacttcatctcatgtggttccagttttttccagagcaccctttttta
gtgaaagaacagctatggtggaccagcatgggaagcatacatacaaggagttatatatcaggagccaagctttatctaaaatgatcatccagcttttggg
tcatccaagtcgtaataatccaccagaaagggtctcttttctctgtccaaacaactcgtcatatgtagtgtgccaatgggcagtatggatgagtggtgcc
attgctgtgcctctctacaagagtcatccaccttctgagctaaagtatattttgcaggattcccagagtgctctggttgtggcagaggaaagttatgcaa
atgtaatgaacccattagcagagcagttgggaattccagtgctgacaatgtctgggtctcagagccttcacccttcagaactgctgcaagaaatcgaagt
atcaaaacttgagttggactggaaagacagaggagccatgataatttacacaagtggaaccactggaagaccaaagggtgttctcagcacccattacaac
ctatattctatggtttcagctctggtcactgagtggggttggacaaaagaagacagcatccttcatgtactgccccttcaccatgttcatggggtggtaa
acaagttgatgtgccccttgtgggtaggagcaacctgtgtgatgctaccagatttttgcccacaaaaggtctggcagcactttctcaggagagatgcacc
ctctatcaacattttcatggcagtgccaaccatctacagcaagctgatttcttactatgagcagcattttacccattccaatgtgcgagagtttgtgcgg
gcagcatgtcaggagagaatcagattaatggtgtcaggctcctcggccctccctgtgccgattttggaacgttggcaagagatcactgggcataccctgt
tggaaagatatggcatgactgaaatcgggatggctttgaccaatccactccatggaccccgtgtgcctggtgcagttggtaaccctctgccgggggtgga
agtacgtactgttacccagaacccacggaaagagggcacctcctacactgtccatgctcatggagattctacaggcaccatggtctctgttggtttggag
aatagagaaggggagcttcaggttcaggggcctgcagtgttcaaagaatattggaacaaaccacatgagacgcaggaagcttttacctcagatggctggt
ttaaaacaggggatacagctgtgtataaggacggcacatactggatactgggccggacatctgtggacattattaaaagtggcggatataaggtcagcgc
cttagaggtagagcgccatctcctgggacatcccagcattactgatgtggctgtgatcggagctccagatgtgacatgggggcagcgtgtagcagctatt
gttaaactgcgagatggacatgcattgtcattgcaggaactgaaggaatgggccagggcagtcatggccccgtactgcattcctgctgagctcatccgag
tggaagaaattccccgaaaccagatgggaaaaatcaataaaaagcagctgctggttcatttctatccacagtaaggagaggactggagtatggagtggag
ccagagtagccccctgcatatacagagagctcttcttgataacactaaaatgatccactattacacctatgcttttatcagcctagtaaatcatgcacac
atagaaccctactgggctgaaatgttattctttctgtataatgttaccatacctgtcttcaacaaagtttcacttgcctatttgtattttactgagaagg
taattatggaagaaatagggcacgaaggcactctgtattggcgtgtaagcaggactctctagcatcccacattttacaccaaaagagacaaatttatctt
ggaaaaagtatccccacccacacaacctaacttatgaatgtacaccagtcttttctggggctaccatcccccatctttctaaacacagtgcaagatatcc
tgtgtatgaatgattttgatcatagatggaaactataaaattattgcagtgataatgttatctttaacttttctctttgactattgaaggcctgtaaaat
ggaccactgtcagtgccatcaggcttagtaaaactgaggtttcattctcaaaaaaaaaaaaaaaa
5' EST: DT412975 [+]ccgagggttcttcactgtgttttgcgttatcctttttgaccctcagtgcttgccgtagttctccacattagtcaggaaggacagaggaaatgttgctgag
aagagtgctgggctagttgaacgagaaagccagtgacaaagccagtcagcagctaccagaccagactgacgagtgaaagctaatatatctcaagtctttt
attggactgactttagaaccagcagtgcaaagaacagaactgacagcagcaggaggccactgatcagcaggcagattgacagccctacagcaggattaaa
ccccatggcatgactcaccgatgacatcagctaactgtactttaggattcataaatgaaattatttgcttttcgcagcctgaggattatagaggcatcct
agactgagccatggcaggagggagctgccattttgtttcacagatgcgggttgcatcctttttggggagatttctttatacttggtcatgtgtccgtggg
gtgcaggtgagaacaaggaccccactgcattcttgctactttcatgtttcaaaatctagcacttcatctcatgtggttccagttttttccagagcaccct
tttttagtgaaagaacagctatggtggaccagcatgggaagcatacatacaaggagttatatatcaggagccaagctttatctaaaatgatcatccagct
tttgggtcatccaagtcgtaataatccaccagaaagggtctcttttctctgtccaaacaactcgtcatatgtagtgtgccaatgggcagtatggatgagt
ggtgccattgctgtgcctctctacaagagtcatccaccttctgagctaaagtatattttgcagga
3' EST: DT412974 [+]gagaatgaaacctcagttttactaagcctgatggcactgacagtggtccattttacaggccttcaatagtcaaagagaaaagttaaagataacattatca
ctgcaataattttatagtttccatctatgatcaaaatcattcatacacaggatatcttgcactgtgtttagaaagatgggggatggtagccccagaaaag
actggtgtacattcataagttaggttgtgtgggtggggatactttttccaagataaatttgtctcttttggtgtaaaatgtgggatgctagagagtcctg
cttacacgccaatacagagtgccttcgtgccctatttcttccataattaccttctcagtaaaatacaaataggcaagtgaaactttgttgaagacaggta
tggtaacattatacagaaagaataacatttcagcccagtagggttctatgtgtgcatgatttactaggctgataaaagcataggtgtaatagtggatcat
tttagtgttatcaagaagagctctctgtatatgcagggggctactctggctccactccatactccagtcctctccttactgtggatagaaatgaaccagc
agctgctttttattgatttttcccatctggtttcggggaatttcttccactcggatgagctcagcaggaatgcagtacggggccatgactgccctggccc
attccttcagttcctgcaatgacaatgcatgtccatctcgcagtttaacaatagctgctacacgctgcccccatgtcacatctggagctccgatcacagc
cacatcagtaatgctgggatgtc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: