XB-CLONE-3151286
Clone Name: IMAGE:7863948
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Gene:
atrip
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125757 [+]
ATGTGTTCGTGACGTCACCTGCGCACCATGAGGAACTAGGCACAGCCTGAGAGCGGAGCGGCGCTGGGAAGATTGTCGCTGCTTTCTGTAAGCGCTCGGC
ATTATAAAGAGGGGGAGCCTGGCAGCCTAAACACGGAGCGCTCAGCAGGACCTTTTATTGGTGCATTATGTCTGTTAACCCCTTGTTTGCTAATAAGAAA
AGGGAAAGCTCCATATTGTACAACACAGCGTGTGCACAAGTCGGCTCCTCTCTCAGTATTCCACAAGGTGCCCAGAGCACCAGCTATGACAGAAGCAGGT
ATCCTCCTCCAAAGCGTCACAGAGCAGCAAATGTTGACTACAAGGCCATGGACGACCCTTTTGATGACAATGAAGATTTTACAGCTGACGACCTGGAGGA
GATTGACATCCTTGCATCACAGGCTTACACGCAGGACACTGGGTCAACAAGTATCCAAAAAACATTTATAAATAACCAGCCAGCATATACATTAAATGCC
ACAAAGCCACATTCTTTATCACACAGAAGTGAAAAAGAATTTCATAAAGACAAATATGCTGTGGAGCTGGAAGTTTTGCAGGCCCATCATGAAGATTTAA
AGCAAAAGCTGAAGGAATTACAAGGTGAAGTACTTGTTAAAAATGGTGAAATCAAGGTCTTGCGCGATTCACTGCGTCACACAGAGTCCAATTTGGAGCA
GCAGAAAATCTCTCATGTGTTGATGGAAAAGGAAAAATCTCAGATGCAAAGTGAGAGGGAGCGAGAGCTTAACAAAAAGATGCAATCTTTGCAATCGGAG
TTGGAGTTTAAAGATGCAGAAATGAATGAACTAAGAACAAAACTACAGAACTGTGAACGCAGCAACAGGACTGCTGTACCACCAGTTAGCCCTAAGAACA
GCCCCTCTATAGGCCTAAAATCAGAGGCTTGTCCATCTCCACAGCCTGGGAGAAAAAGCTTTCCCACAAAAGAATCATTCTGCTCAGATATAAATCTCAG
AACCCCCACATTAATTTCACCTCAGATTGGTTTTCGAGTTCCAGTAACTACCAAAGAACCTGAAGTTTTACCTATATCAGCGAAAGCTTTCTCTTCTTTG
TTCTATGCCGAACGAAAAAATAGCCAAGGATCTCTTTTGCTCAACGCTTTGATGCAACAGCCCATAAGCCCAGGATCACTTGGCCTCTGTCACCTTCTCA
GTAGCAGCACAGAGTTGTTCCCTGGCTCTCCTGTACGGAATACCCATTCAAAAAATGCAACATCCTGCTCCAGTGTTTTGTCCACGGCTCAGTGCTCTGC
ACTAAGAGATGCTCAGAAATTAGCCCTTACTGGACTTAATGCCATTGCAGTGGGTGAAGTTATGCCTTGCAAGAGGGAGACGCCAAAGCAGAGTGGGTTA
TTACACCTGAACAAGCTGAGTCCCATCGCAGGGGCTGTACATCTTCTGCCTCTAGTTGAATATCACATTACTGCATACTGTCAGGCCCAGCATACCTCTG
AGAAGTCTGGATCAGGCCCCTCAGTAACCCAGTCTATGTCTTCCTCCAGAACAGAGCAAAGTCTGGCCTCCAGTGTTGAGGACATCCTCTACCATCTTGT
AGAACCTGCCTTGGCCTCTCTTGGGATACTTTATCATCTGGTATTTTACAGTCTGGAAGCAGTAAGCTCTTTATTGAGGTGCACAACACATTCCTCTGAG
GCTGAGCAGGAATCAAGAACTTCTAAATCTAATGCCGATGTTGACTGTGGTGATGACCAGAACTGTGATATTCAATGTCTGCATCCTTTGTTTGATAAAC
TTGTCCAGCTCTTGTGCTTCAATGTTGCCACATGCCACAGAGACACTGTCTGGCACCAAACTCTGCGCGTACTGGTAAAATTGGCTGAAAATTCATCCAC
TGAACTGTTATCCAGCTTCCAGCACCTATTAAAAAAGCCTGCATTACTACTTTGTCTGGCCATTGAGTCTCCTGTTTCTGTGGCCCACATGACAGTTCTA
CTCCTGGCTGTATTAGCTGATCACCAGAAGTTGTCTTCCCTGTTCTGTTCCTGCTCAGAAACCTGTATCCTGCTTGCACTCTATACGTACATCACTTCCG
GACCGGATAAGTCTGCATCAGAATCGCTTTGGCTTCGGTTGGAGCATGAGGTGGTCCGTTTCCTTACCAAACTGTCTGTGATAGGCTGGAACCCAATCAA
CACCACCTCAGAGACTGTATGCCAGTGTGAGCGGGAGGTTGTTAAAGCAGTGGTCCTGATGCTGCACCGGGAGTGGCTGGGTTTGCGGCGCCTGTCACTC
CATCTGCTGACCACTACTAAAAACAAGGTGGTGCAGTTCTTGCGGGACGCTGTGCTGCTGCTGCACTCTCTTTCTCAGAAGGACAGGAACTTTCATGAGC
ACTGCCTGGAGGTCCTGCACCAGTATGACCAGGCCTTACCTGGGGTCCGGGCTCTCTTCAAGAAGGTGCAGGTGTTGAAAGAAAATGAAGAATATGCATT
GGATGAGCTCTGCCCCCCGGAAGTTGAAACAGAGGATGAATACATGGATTGCACATGACTGCTACCTTGGGTCTTCCTATAAGCTGTGGTCTCTTTTAAT
GAACTTTTTATGTCAGTGCAGTTCTCTCTGCAAACCCTTTACTATTTGTTTTCAGTATTGTGTAGCTGTAAATACTGTACTCTAAAAAATAAAATAAATT
CCAACCTTGTACAAA
5' EST: DT413302 [+]atgtgttcgtgacgtcacctgcgcaccatgaggaactaggcacagcctgagagcggagcggcgctgggaagattgtcgctgctttctgtaagcgctcggc
attataaagagggggagcctggcagcctaaacacggagcgctcagcaggaccttttattggtgcattatgtctgttaaccccttgtttgctaataagaaa
agggaaagctccatattgtacaacacagcgtgtgcacaagtcggctcctctctcagtattccacaaggtgcccagagcaccagctatgacagaagcaggt
atcctcctccaaagcgtcacagagcagcaaatgttgactacaaggccatggacgacccttttgatgacaatgaagattttacagctgacgacctggagga
gattgacatccttgcatcacaggcttacacgcaggacactgggtcaacaagtatccaaaaaacatttataaataaccagccagcatatacattaaatgcc
acaaagccacattctttatcacacagaagtgaaaaagaatttcataaagacaaatatgctgtggagctggaagttttgcaggcccatcatgaagatttaa
agcaaaagctgaaggaattacaaggtgaagtacttgttaaaaatggtgaaatcaaggtcttgcgcgattcactgcgtcacacagagtccaatttggagca
gcagaaaatctctcatgtgttgatggaaaaggaaaaatctcagatgcaaagtgagagggagcgagagcttaacaaaaagatgcaatctttgcaatcggag
ttggagtttaaagatgcagaaatgaatgaactaagaacaaaacta
3' EST: DT413301 [+]tttgtcaaggttggaatttattttattttttagagtacagtatttacagctacacaatactgaaaacaaatagtaaagggtttgcagagagaactgcact
gacataaaaagttcattaaaagagaccacagcttataggaagacccaaggtagcagtcatgtgcaatccatgtattcatcctctgtttcaacttccgggg
ggcagagctcatccaatgcatattcttcattttctttcaacacctgcaccttcttgaagagagcccggaccccaggtaaggcctggtcatactggtgcag
gacctccaggcagtgctcatgaaagttcctgtccttctgagaaagagagtgcagcagcagcacagcgtcccgcaagaactgcaccaccttgtttttagta
gtggtcagcagatggagtgacaggcgccgcaaacccagccactcccggtgcagcatcaggaccactgctttaacaacctcccgctcacactggcatacag
tctctgaggtggtgttgattgggttccagcctatcacagacagtttggtaaggaaacggaccacctcatgctccaaccgaagccaaagcgattctgatgc
agacttatccggtccggaagtgatgtacgtatagagtgcaagcaggatacaggtttctgagcaggaacagaacagggaagacaacttctggtgatcagct
aatacagccaggagtagaactgtcatgtgggccacagaaacaggagactcaatggccagacaaagtagtaatgcaggcttttttaataggtgctggaagc
tggataacagttcagtggatgaattttcagccaattttaccagtacgcgc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: