XB-CLONE-3145680
Clone Name: IMAGE:7866467
|
Gene:
as3mt.1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC169152 [+]
ccagagataagtggagccgctgcctcccccaaactgtcaccttctattgtgttcccagctttaagcatatgtgacccttggacctacacaggctgtgaaa
tcagctccggggagtatgcagtgagtagagagaaggaagataggagttgcagagctcttctggaagccataatttctacaaagcagaccctcttgtgtaa
tcagcatgtgcagtgtcctgcagagcacagagtgtgactgcagcacacaaggacttcagacgtacgaagatgtcaaggaatattatggaaaattcctgaa
aaattcaaaggatctgaaaacaaatgcttgcgtggctccttcaaggcagttaccgaaacatataagagatgccttgaaagatgttcatgaagatgtttcc
tcacggtactatgggtgtgggcttgtggtaccagaatgtttggagacctgcagtattctggatctgggcagtggaagcggaagggattgttacatgctta
gtaaattggttggacagaaaggtcatgtgacaggaatagacatgaccgatgagcaggtggaagtgtcaaaaaaatacattgactatcacatgcaaaagtt
tggtttccagccagccaatgtggattttgtgcagggttacatagaaaatctccatgctgtgcagattaaagacaaatgctatgatgtcataatatcaaac
tgtgtgataaacctttcgcctgataagagggctgtgttgaaagaagcgtacagagtattaaaggatggtggtgaaatgtactttagtgatgtttattcca
acagaccggtttctgaggaactgaaaaagaacaaaatcctgtggggtgaatgtctcagtggagcattatgctggaaggagcttgtgcagatttcagagga
gattggtttcagccccccacggttggtaacctccagttttatcaccgttgacaacaaggagctggaggagattcttagtgactacagctttgtatctgcc
acctaccgcctttttaaactgccccagggcaaggcgaaggaaaagtggcaagttatttacaatggagggatcacaggctgtgaagaggagctgcagttcg
atgcaaactttactttcaaggaaggggaagttgtaaaagttgatgaggaattgggtgcgatcctaaagaactctaggtttgctgatgaatttttattccg
cccatgtgagaagggagacctatccagcaaatgccagccaaaacgggaaatcattagggaccctttccagcttgcccaaaaggcgaggaaccagcatcag
gctcctaattctggaagctgctgtggcccaaaaggatgttgctagggatgggtgagtggttccgaagatcattagacgtcacccaccaacagatctttaa
ggcgcttactaaatctcttctggcattcctgattcttacatagtgtaggggtcacttacctcttttatattaattggtatttgtataatgtgtattgtat
atgtgtttgtatgcaccctgttatacagtgttgtggaatatgtttcaagtctgctccttcagtagggaggcccgaaggtgacccatgaaagcaccgaatc
tgtagcacatggggcagtgattggctgaacgtggcaccataactgccatgcaggaatcctagcactcggcattccctattctgcgactccccaccccaga
cacccagctagcttccctccagctccagtctctataacccacaactccctgaaccttcactgacacaatatggggcaatctgattgtaacagaatttaat
ggtgtattagcataatgtgtgatttataacagtgtaagtgttagcatatgaattgtatcactaaaaaaagtaaagaaaatggaaattattataccatttg
ctattaaataactatgtttgaaaaaaaaaaaaaaaaaa
5' EST: DT416999 [+]ccagagataagtggagccgctgcctcccccaaactgtcaccttctattgtgttcccagctttaagcatatgtgacccttggacctacacaggctgtgaaa
tcagctccggggagtatgcagtgagtagagagaaggaagataggagttgcagagctcttctggaagccataatttctacaaagcagaccctcttgtgtaa
tcagcatgtgcagtgtcctgcagagcacagagtgtgactgcagcacacaaggacttcagacgtacgaagatgtcaaggaatattatggaaaattcctgaa
aaattcaaaggatctgaaaacaaatgcttgcgtggctccttcaaggcagttaccgaaacatataagagatgccttgaaagatgttcatgaagatgtttcc
tcacggtactatgggtgtgggcttgtggtaccagaatgtttggagacctgcagtattctggatctgggcagtggaagcggaagggattgttacatgctta
gtaaattggttggacagaaaggtcatgtgacaggaatagacatgaccgatgagcaggtggaagtgtcaaaaaaatacattgactatcacatgcaaaagtt
tggtttccagccagccaatgtggattttgtgcagggttacatagaaaatctccatgctgtgcagattaaagacaaatgctatgatgtcataatatcaaac
tgtgtgataaacctttcgcctgataagagggctgtgttgaaagaagcgtacagagtattaaaggatggtggtgaaatgtactttagtgatgtttattccn
acagaccggttttctgagaactganaaagaacaaaatcct
3' EST: DT416998 [+]caacatagttatttaatagcaaatggtataataatttccattttctttactttttttagtgatacaattcatatgctaacacttacactgttataaatca
cacattatgctaatacaccattaaattctgttacaatcagattgccccatattgtgtcagtgaaggttcagggagttgtgggttatagagactggagctg
gagggaagctagctgggtgtctggggtggggagtcgcagaatagggaatgccgagtgctaggattcctgcatggcagttatggtgccacgttcagccaat
cactgccccatgtgctacagattcggtgctttcatgggtcaccttcgggcctccctactgaaggagcagacttgaaacatattccacaacactgtataac
agggtgcatacaaacacatatacaatacacattatacaaataccaattaatataaaagaggtaagtgacccctacactatgtaagaatcaggaatgccag
aagagatttagtaagcgccttaaagatctgttggtgggtgacgtctaatgatcttcggaaccactcacccatccctagcaacatccttttgggccacagc
agcttccagaattaggagcctgatgctggttcctcgccttttgggcaagctggaaagggtccctaatgatttcccgttttggctggcatttgctggatag
gtctcccttctcacatgggcggaataaaaattcatcagcaaacctagagttctttaggatcgcacccaattcctcatcaacttttacaacttccccttcc
tgaa
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: oviduct | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: