XB-CLONE-3136928
Clone Name: CAAO2476
|
Gene:
mgrn1
,
igfals
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX932697 [+]
tgagccgccgcctcccgctgctcggtctctatggctggatggaaacaacctcacaacggtgcaggctggagctttcaggaatctatcccatctggacttt
ctcaacctgcagagtagccaggtggctagcctagagcaacaggccctgcatggcctcaaggctttggctcatctccacttggaaaggaacatgatcaaat
cattagctccaagcactttcactcatactcagaatttggtctccctcacccttaacaacaactttttgaccaagatcgaagacggtctgttttctggtct
ctccaacctgtggtacctgaatttaggttggaacctgttagtggtgcttcctgacatggttttccacgacttgctgaacctgagagaactggttttggct
ggaaatcgccttgtgtacctgcagccttcactcttccttagtctcggggagctaaaggaactcgatttgagtggaaactcgctgaaaggcattaaggcta
atgtgttcacgaggcagcaaaagacacacaaactttatcttaaccacaaccacatttctaccgttgctccgagggctttctctggcatgaagaacctacg
ttggctggacctgtcccacaaccgactgaccgctttgtacgaggacaccttttttgggctgccgagcctcaatgttctacgtctgtctaataattctctc
acaagcctaaggcctagaattttcaaggatctccagttcttagtggagctcaaccttgggcaaaataaaatcaagatcctcctggaacgtacttttgaag
gtctcgctcagctggagttactatcgttaaaccacaataacgttcaggaaatccgccagggtcgttcttggggctgcttaatgt
3' EST: CX932696 [+]caggcagtaaatagctttttatttcattttttaatacaaagaagacaacagtgaggcaatgtgtattgtgggtcgtattctactcattatactgtcagtg
atactgggaatgatgggccaatgactgcccttctgctattccccctacttacaatacttgcactgttctgcatgtgggccccttgggcatggtgagagaa
agtatcttgactgccagatatatacatatacactcaattacctgtatattccacttcatattaatgaaaggctccaccctttcccccactggctcctccc
ctgtaaccatcatagcgcgggtctgatatacagggcatgtctggagaatctagcgaagctcatactggctattctacacggctgggcctcatcctaacaa
catattagtgtggcaatagaagctgtgggttgtatttgccaatgtcttagggttacaagcagtgggcaaagtggccttcactcagatcccgtatgtcctg
gcccatggcttccggagggctggcacatgtgatgttattatagacgggactggcagtgtcgtcgccttctgatattgactgtaccaggtgagggacgatg
gtgctgttcctcagagcaaggtctcgtagtcctttcagagcgcagccacagtcccattgattgccattaagccaaagctgctgcatggtgggaggaggta
tcaggaagttaacagagatggttttcaggcagttgttcttcagactgagatat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: