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XB-CLONE-3135662
Clone Name: CAAO3608 | Gene: cyb5d1 | Species: Xenopus tropicalis | |
Sequence:
5' EST: CX934063 [+]
Library:
Name: NIH_XGC_tropTe5 | External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library |
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.
Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map | Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: SalI 3' Restriction Site: NotI |
Usage:
Suppliers:
Search for clone at: Open Biosystems