XB-CLONE-3134336
Clone Name: CAAO4360
|
Gene:
gas8
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX935416 [+]
taaagagtagccaagatgccacccaaaaagaagaaggaagctgggaaaaagaagtcagctaaagggaaaaaaagtacagctgtggtagatggcattgcca
cagaggaaatgagcaaggaagagctggaggaacatattgtgcgtcttcgagaagaactagatcgggaacgagaggaacgtaattatttccaactagaacg
tgacaagattcacaccttctgggagatcacaaggcgtcagctagaagagaaaaaatgtgaactacgcaacaaggaccgtgaaatggaggaggctgaagag
agacatcaggtggagataaaggtgtacaagcagaaagtaaaacatttgctgtatgagcatcaaaataacatctcagagttaaaggcagagggaagtgtat
ccatgaagctggctcagacagagcaccatactcaggagagcttgctacgtaaggatatgcgtggactaaaggtggaaatcaaagagcaagagttggccaa
tgaaatggttgtcaagaaccttaaaatgaaacaagatgaagagaccacgcgattaaggaacgactttgaaagacaagtacgagaaattgaggctaagtat
gagaagaaaatgcgcatattgcgagatgagctggaactacgcaggaagacagaaatccatgagattgaggaaagaaaaatggacaaatcaatacactcat
gcgtaaccatgagaaggcattcagtgacatcaagaactactacaatgacatcacactcaataacctagctctcatcaattcacttaaagaacagatggaa
gagatgaagaagaaagaagatcgca
3' EST: CX935415 [+]agaaattgatattctttgtattgtaagtttgaacagaaggaaaccagggatatttttacaatggagataaaactctgcattacgtaggggcagaaaccag
tcctgcagggccttgtccaaaattctgcccaggcaccaggcattccagaggtttgaagccaagctcatcagcaggaatcccaaaggctctcatcttcgcc
tcgtatgttccgagcagatcattgtgtgccttgcaaactcttgccagctcatactgcaaatccttgatggcaccattttttgaatccaaaacatcctcca
gtttgcgtgtgacaactgcaagtgccgtgggatctaggttggaggcagcaagcacctcattaagttgtgcctccttcttttctagagcatcacctagagc
ctgcagtttgcgttccaacaacaggttcttaaaaccacttttctgttgaacctcatgaatggcagctgtgaatttcttataaagctcatcccgctccatt
tgcaccttttggaacctctgttctagaacctcatgttcccatttcaggtcattcagttccttctctgttgctttcaggcgagcctttgtatttgctagtg
ctgtcttatctttctcatagttggctagctgcctgttgagctcggtaacctcttctctggccttctgcagaggttcagtaagacgacggttttgaagctg
taagtcagccatctccttttccatgcgatcttctttcttcttcatctcttccatctgttctttaagtgaaattgatgagagctag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: