XB-CLONE-3132955
Clone Name: CAAO5157
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Gene:
manf
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX936864 [+]
gaggttgctaggagaagcggaggcgcagttgccggctggttgggcgaacagaccgggggctttgtgccacgtgcctatttcccggcggatccgttgctcg
agtgttgggaaggaagatggttcctctcgcccttattactgtgactgggatgctggctcttctgccaagcgatgctgcagctctgaaagccggagattgc
gaggtgtgcatttctttcatgacccggttgtaccagtcccttaaagaacggaaagtagagtttaagccagacgttgttgaaaaggaactgctaaagacct
gcaatgatgccagaggaaaagagaacaggctgtgttactacattggagccaccagcgacgctgccaccaaaatcacaaacgaggtgtccagaccattaag
taaccacatccctcctgagaagatctgcgagaagctaaagaagaaagatgggcagatctgtgagctgaagtatgataaacagattgacctgagcacagtg
gatctaaagaagctcaaagtcaaggaactgaagaagattttggatgactggggcgagtcctgcaagggctgtgctgagaaatcggactttatccgcaaga
ttaatgaactgatgcctaaatacgctcccaatgcagccaatgcacggactgacctttaaagcgctatcctgtggatctagtgcaagaactgatcctctaa
actatagacccagccagcagacgttcctctaatatggttatcatttttactttttaaggaacagaatttttttttcactttagntctgttttcttttttt
tttt
3' EST: CX936863 [+]aagaaacaaaactgtttaatttcaacagcaagacaggggcaaatgtgttatcactgtttttgaacattccagtagctggagggtgccaaaaaggttcagt
gctaatgtgtaaagcaaaaaaaccctgggtgagttttcctcttggggcaaaatactgctgattacactaggtaacctgctctataccagtggagcctgca
gctgtgtgcttagcacaatgggacatctgtacaaaagcccagcctttcccccagtctgccttattgctcatccagctgcgtgacacctgatatgaagcag
agggggcaactgtaacgcagttcttgccttcattcattttttttttccccccagcaagaggcagagtggatccttttattttcataaaaaaaatgcccct
caacaagaacccaaagtaacatggaggtaataaaaattccccgtactgcaagactgtccgagacagcgcaaagaaaaccaataagttacaagcaaatgta
tcaaacaaaagtgtcccacctgactgcagggttcatattcctgcaatctgggggagtcttcagacttttacaaaaaaaaaagaaaaaaaaaaaaaaaaag
aaaaccg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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