XB-CLONE-3129590
Clone Name: CAAO4341
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Gene:
chd5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX935383 [+]
gactggcagagactttggtaataccatggccgctgctagaaagttttttagactacttcttaaaccatttaccctactatgtaacatcaaatgtagaaga
aaaaaatcaaataatgataacagtacaagttgtagtgaagaaaacaaagattcaaaggtttttaagaaaccaaacaaattagaatcagaggaaacactac
aaatttgcatcaataatgatgaaccaaacaaggccttaagcatctgtgtaacagatgaagagagaaatgaggtctctgttccctgcaaagcagagcaaga
aggaagcaaagacttcagtgcctgcgtaactgtggaacaagtaagcgacctctccaccctctgtttaaaagaggaatatgctgaaaaggcagcaaatgtg
gaatgtgaagaaaatcaaaatgacacttcttgcaaaacagagcaagagacaagcaatgatgccaccctttgcaaaaatgaacaggaaaacaaaccagaac
aagaagagccaaatccctgctccaatgaggaacagagaaatgaggctactacttcttgcttaaaggagaaaaagaccagaaaggtggcaagaaaggggaa
gagaaataagaagcagatccccaaggtggaaataaaagaaaacccctctaatctttgcctaaaggagcaaaatagtaaaaagatggcaagaaaagagagg
aaaaataagaaacgccttatncaggcagaaatagtgaaaaacacaccaactgactatgaaggattatacagacccctatatgactgtatatataaaggag
agagaaacgagac
3' EST: CX935382 [+]ataaaaatattttattaaaaaaataaatatacatatatatatataaaacattataataatacagttcacttctgtccaatatcaaagtcactgggagaat
atattgattggctggtcttgagtataagttatatacagtctctccatcttttgggatagtatatgagcataggttctacatcttttgtctcattttctat
tccagccatgtaaaattaagtgtgggctccaagtcaaccacacaagcatctgcttttttaggaaatgcagtaaactttcttctctctaaacaacgaatga
gatgtcctctcattttcttgcaatcatatttgcagattgggtttccgccagataggaacgcaaatgcatttgcaatagcataaaccccacagtcataatt
gttttcttgttcatcgacttcaagtatctccatagttccttcagggtcattcaccagtgcactataacaatcctttatttgtttccttagtgaggtgcaa
tggtgttttgtcttagcactgtttgcgatctcaacatgatccattgcaaagcagcttgtcagccagtgcttctctacgctgtcataatgaatctgcacag
agggtcctttaactggattaaatccaatttgggaaaggaggcaactttggagcccctctgccccaaactggattttcagcaaggactgagctgcatcgat
gattctgtcatccagacattctctacctcgcaggtcgtttttatgctgctgcgtcaggttgagttcagggatccagttatccgtccctat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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