XB-CLONE-3129275
Sequence:
5' EST: CX935949 [+]
caggacgcgacttgcgagagcgtttcgtgttcgttatacagactgaccggtgctggctggcgtgctgacagcttattaagaatcagcatcacaatggcag
tcaggaatgtaagtacaaaggttccagtgggcacagggttttggaacaatgccaaacctgctcaatacagcaaagaaacccaggagctgattcgagtgat
gatggaagaatccaaacttacaaactttcaaaagcgtcagataaaagagcggcttcaaggcggcgatgcactaccagttcagtgccaccccagttccact
gattctggtagtaaacgtgcagcagctgcaatctcccagagaccaaaagctacccttcagccttgcagaccaagattgcgtcctgctgagaagtgccgtg
ctggagatgcttattgtagagacaagttccagcctcggcccacaagagatctgaataaggaaaagtgcaggttacaaaatatcatggcaacagggaagga
ccttcctgcaccaacacgtgttattccacaaaaagaaaactgggaggaagaaggggagaaggacagatttgatgaattggtggcagaagtccaggagaga
tgggacttccttgaagaaatggaagccttaggtaaagggaaagattaccgaaatataatcaacacggaaatctcacagaagttgcgtgagatggaaatcc
ttgataaacagaggagcacggaactgaaggagctactgcaaaagtcagagaacaagcaattgtcctaacatgtcctagcagtgtattgagcctacttggt
gaccacagataanacattcactgcccat
3' EST: CX935948 [+]ccactatttgcaccctcccaattatagaagtcaagaaacaaagaaaatggctcacctgttcaaaattttggatttactgtattggattgtgcagaacaca
cattgcaatcagtgtaacttctgtaaaaatccaaccatcttatctggccagtaaaaagcaatatataatttattaactgaagcaataagaaaagggcaaa
cccacaggcacaagtaggtggggacaaagagtcagacattcaaaatatgggcagtgaatgttttatctgtggtcaccaagtaggctcaatacactgctag
gacatgttaggacaattgcttgttctctgacttttgcagtagctccttcagttccgtgctcctctgtttatcaaggatttccatctcacgcaacttctgt
gagatttccgtgttgattatatttcggtaatctttccctttacctaaggcttccatttcttcaaggaagtcccatctctcctggacttctgccaccaatt
catcaaatctgtccttctccccttcttcctcccagttttctttttgtggaataacacgtgttggtgcaggaaggtccttccctgttgccatgatattttg
taacctgcacttttccttattcagatctcttgtgggccgaggctggaacttgtctctacaataagcatctccagcacggcacttctcagcaggacgcaat
cttggtctgcaaggctgaagggtagcttttggtctct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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