XB-CLONE-3117805
Clone Name: IMAGE:7872196
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Gene:
rpap3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121516 [+]
TTTAGGATCATCTGGTCTACAGAATGTCTTCCCCAAGCAAAGCAATTGAACTGCAGCTACAGATGAAACAAAATGCTGAAGAACTGCAAGATTTTATGAG
GGACTTGGAGAACTGGGAAAAAGATATCAAGCAAACAGATGCCAAGCTAAGAAATCAGACAGGCGTGGATAGCCAGATATTGCCTCCTATCAGAAACAAA
GATTTCAAGAAAAAGAAGAAGAGCAAATCTAAACCACCACTTGAAAAGAAGAGCCAAGAAGATGAAATAAAACCCAAGAAAAAGTTACTTGATTATGAGT
ATTGGGACAAACTTGATGTGGACAGAGCTCTAGAAGATATAGATAAAGACAACAATGAATCGTCATCGGAGTCTGAATGTGGGGATGAAGATGGAATAAC
TGTAGACACAGAGAAAGCACTTTTAGAGAAAGAAAAGGGAAATAATTATTTTAAAAGTGGACAGTATGATGAAGCTATAGAATGTTATACTAGAGGAATG
GATGCAGATCCCTATAATGCAGTTTTACCAACAAATCGTGCATCTGCCTTTTTTCGACTGAAAAAATATGCTGTTGCAGAATCTGACTGTAATCTCGCTA
TTGCTTTAAATCATAATTATGCAAAAGCATACGCTCGTCGAGGAGCAGCTCGTCTTGCACTAAAAGATCTACAAGGAGCAAAGGAAGATTATGAAAAAGT
TTTAGAATTAGATGTCAACAATTTTGAAGCCAAGAACGAACTAAGGAAAATTAACAAGGAGCTGCAGTCTTCCACTAGTGATGTCCAAGAAAAAGAAGCC
ATAGAAGAAAAAATTACTGTTGAAAATGAAGAAGAAAAGAAACAAATAGAGATTCAGCAGCGGAAGCAACAAGCTATTATGCAAAAAGATCTGGGCAATG
CATATTTTAAAGAAGGCAAGTATGAAATAGCTATTGACTGTTATAGTCAGGGAATGGAAGCAGACACCACAAATGCATTATTACCTGCAAATAGAGCAAT
GGCATACCTGAAAATTCAAAAGTATAAAGAAGCAGAGACTGACTGTACACTAGCCATATCACTCGATGCATCTTATTGCAAAGCTTTTGCTAGAAGAGGA
ACAGCCAGGATAATGCTTGGAAAACAAAAGGAAGCAAAAGAAGATTTTGAAATGGTTTTAAAATTGGATCCTGGAAACAAACAAGCTGTTCTTGAACTGG
AAAAAATATCTCGGGAACTAAGGAGCAATGAAAAAGATACAAAAGGAAATAAGGAGAGAAAGTTAATAAACGCAGTTGAAAAGCTTCCTCATCAGAGGTC
CACTAAACCATTAAGAAGGATGGTAATTGAAGAAGTTGGAGGCCCAGTTGAAAGTTGTATCAGTAGCCTAAATGAAAGTAACCACGGGAAAGCAGACTCT
ATGGATTTAATAACTAAGGCCGATAAACAAGATCTGAACGAAGAACAGAACTTTTGCAGCTTACCTGATGTTCCCAGTGCAAAGGTCCCAAAAATAGAAG
AAATCAGTGATACATATGGTTCTTGCGAACCATCTTCTGGAGAGGAGCACTCTGTATCTCAGCCGTCGCCTCCAAAAATCGAAAAAGTTGTCACAACATT
TTCTGAGTCTCTGAATATAGGGATTCCTGTAGTTCCTACAAACTCTTTCCAGCTTGAGTCGGACTTCAGAAGATTGAAAGGGAATCCTGACTTATTGTAT
AGGTACCTAAAACAAATTGAGCCCTCGTTTTATGGTAAATTGTTCCAAAAAGCTTTGGATCCAGACCTGTTCAGTGAAATTCTGACAATTCTCAGAGAGC
AGTATATTAACAAAGACAGCTGCGATCTTATCTTTGAAATATTACAAAGGCTGTCTGAACTGAAGAGGTTTGACATGGCTGTAATGTTCCTCTCAGGATC
AGATAGAAATAATGCCCACATCTTATTTTCACACCTAGAGCAATCGCTGAAAGGCAGTGTCTCTCTTAATGCTTTGAAGAAAAAATATGGCCTTTAAATC
TGTGCTTGTAAACAGAGGACAGTCTGATGAGGCTATAAAACTGAAAAAGAAGGAGACCATCTGAAGCCAAAACTCAGTGTTACTAACAGCAGGGGCAGAT
ACCATATTATTTTTGTATACACAGATTATGAGTGCCTGCTGGTTAAATTATTAAAATATACTATTTATTCTCCCAGTACGTGCAATTAGTTCTCCTTCCA
AGCCCTGTAGTATTTCATCCATCCTTCATCCATACAGCAATGTTTGCCAACCATACCAGTGCTAATTGGCACATTGTAACAGGGCATGTGCAGAGCCGCC
TGTTCTGGCTTGTGGGCTGAAATGCGCTTCTCTAAAATCTTCTGATGAAATTACATCCTGGAGAGTTGGGAAGAACTATTTAGAGCTCTTTATTTGTTAC
AACTGAATCACCAGACGCTCTCTAAAAAATAATGTAGACCTGCCCCAGAATAAAGCATTTTAAGATGGCTAGAATTTTTTTTAAAATGTATATTTCTGAG
ATGCTTATGAGTTTCAGATTTAAGTTGACAGGATGTATACAAAATAAATGTATTTTTCCTAACAAAAAAAAAAAAAAAAAA
5' EST: DT427972 [+]ttaggatcatctggtctacagaatgtcttccccaagcaaagcaattgaactgcagctacagatgaaacaaaatgctgaagaactgcaagattttatgagg
gacttggagaactgggaaaaagatatcaagcaaacagatgccaagctaagaaatcagacaggcgtggatagccagatattgcctcctatcagaaacaaag
atttcaagaaaaagaagaagagcaaatctaaaccaccacttgaaaagaagagccaagaagatgaaataaaacccaagaaaaagttacttgattatgagta
ttgggacaaacttgatgtggacagagctctagaagatatagataaagacaacaatgaatcgtcatcggagtctgaatgtggggatgaagatggaataact
gtagacacagagaaagcacttttagagaaagaaaagggaaataattattttaaaagtggacagtatgatgaagctatagaatgttatactagaggaatgg
atgcagatccctataatgcagttttaccaacaaatcgtgcatctgccttttttcgactgaaaaaatatgctgttgcagaatctgactgtaatctcgctat
tgctttaaatcataattatgcaaaagcatacgctcgtcgaggagcagctcgtcttgcactaaaagatctacaaggagcaaaggaagattatgaaaaagtt
ttagaattagatgtcaacaattttgaagccaagaacgaactaaggaaaattaacaaggagctgcagtcttccactagtgatgtccaaganaaagaagcca
taga
3' EST: DT427971 [+]gttaggaaaaatacatttattttgtatacatcctgtcaacttaaatctgaaactcataagcatctcagaaatatacattttaaaaaaaattctagccatc
ttaaaatgctttattctggggcaggtctacattattttttagagagcgtctggtgattcagttgtaacaaataaagagctctaaatagttcttcccaact
ctccaggatgtaatttcatcagaagattttagagaagcgcatttcagcccacaagccagaacaggcggctctgcacatgccctgttacaatgtgccaatt
agcactggtatggttggcaaacattgctgtatggatgaaggatggatgaaatactacagggcttggaaggagaactaattgcacgtactgggagaataaa
tagtatattttaataatttaaccagcaggcactcataatctgtgtatacaaaaataatatggtatctgcccctgctgttagtaacactgagttttggctt
cagatggtctccttctttttcagttttatagcctcatcagactgtcctctgtttacaagcacagatttaaaggccatattttttcttcaaagcattaaga
gagacactgcctttcagcgattgctctaggtgtgaaaataagatgtgggcattatttctatctgatcctgagaggaacattacagccatgtcaaacctct
tcagttcagacagcctttgtaatatttcaaagataagatcgcagctgtctttgttaatatactgctctctgagaattgtcagaatttcactgaacaggtc
tggatccaaagctttttggaacaatttaccataaaacgagggctcaatttgttttaggtacctatacaataagtcaggattcccctttcatc
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1562 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: skin | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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