XB-CLONE-3105878
Clone Name: IMAGE:7876378
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Gene:
ythdf3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161192 [+]
gcggtacgttcaagcagcgatccgggactgcggactgtgttaagaaatatcgggactgtcccgcgaagagcgggactgtggggcggtatctgaacggggt
cagccgattagcagctctcacatgtgaatgccgggagcctatagttgaacgtcccatcacagccggtagtggctctccctgtgtcctgcagtggcagtcc
ttacacgtacgtctttgtactacacgtgtgtgtgtgcgcatctcactctcttaaggggactctttggttttttccttctttttattactattgttattga
gattttatttttaatactgggttagcaaagaggggatttcaaggcggctcaggatttttttctggctccacaggagaatgtctgcgaccagtgttgatca
gagacctaaaggacagggaaacaaagttgcagtacagaacggttcgatgcatcagaaagatgcagtaaacgatgatgactttgagccatatctaaatagc
caaacgaatcagagtaatagctatccaccaatgtcagacccatacatgcctagttattatgctccatccattggatttccttactctctcggtgaagcag
catggtcaacagctggtgacccaccaatgccatatttatacggacagatgagcaatggggaacaccactacataccagacggagtgtttagtcagcctgg
tgctttaggaaacactcctcccttcctcagccagcatgggtttaatttttttcctggcaatgcagatttctcaacgtgggggacaagtggatctcaggga
caatcaacacaaagttctgcttacagtagtagctacggatacccacctagttctcttggtagagccatagctgatggacaagctggatttgggagtgacg
ctctgagcaaagtgccaggcattaatagcattgagcagggcatgacagggctgaaaattggtggtgacatgacagctgctgtaacaaaaactgtaggatc
agctttgtctagtgcagggatgacaagcatagcagcaaacagtgtacctccagttagcagttcagctcctaaaccaacctcttgggctgctattgctcgg
aagccagcaaagcctcaacctaaattaaaacctaaaaataatgtgggtattggtagctctgctgttcctccaccgcctataaaacacaacataaatattg
gaacttgggatgataaaggggctattgtaaaaacacctcccattcaatcagttctgcctccacagcctgtaattcaacagcctcagccattaactcaacc
tctacccatggtgcaaaaccaactgcctcagcagcagctacagcaacaaggacctcagcagccacctcaccagattcagcagcagcttcaaaaccgctgg
gtggcacctagaaacaggggggcgggcttcaatcagaacaatgtatctggcaatgagaattttagtttaggtgttgtgcctgttagctcttcgccttctg
gtgttgaagtacacccagttctggagaaactgaaggccattaacaactataatcccaaagactttgactggagtctaaaaaatggacgtgtgtttattat
caagagctactctgaggacgatattcaccgttctataaagtacacaatctggtgcagcactgaacatggcaataaacgtttggatgctgcttaccgatcc
ttgaatggaaaaggcccactttatttactcttcagtgtaaatggaagtggacatttttgtggtgtagctgaaatgaagtctgttgtggactataatgctt
atgctggagtctggtcccaggacaaatggaagggaaagtttgatgtcaaatgggtctttgtcaaagacgttcctaataaccaattgcggcatattcgttt
ggaaaacaacgataacaagcctgttaccaactcaagggacactcaagaggtacccctagaaaaggccaagcaagttcttaaaataattgctacattcaag
catacaacctctatctttgatgactttgcacattatgagaagcgtcaagaagaggaggaagccatgcgtagggagaggaatagaaacaaacaataatcat
acagcgatatcctgctataatcacaacactaatgatgtagactctggaaacgtatactatgttgaagaatacatttaatgctttttatttctgctttggt
gagatcgttggacactttaacacaaaattgacttttttttcccccctcctcataatggtttcatcatctgccctgatgctctctcaccaaacacacttga
gaactttaactttgtcaagcactttctgtcctgatgcttttaccagtatctgctgtcttttttgtaatcatgcatcctagctaaatctctggagactcat
tgaatgcaaggttccattgttttaaaattgtttaaaatactaaaatcttgttagaaaagttgttgatgtgcgattcacaatgttaagtttttctttatgc
attttatggtgtcctattagatgtggaagattaaaaaaaaaaaaaaaaaa
5' EST: DT435832 [+]cggtacgttcaagcagcgatccgggactgcggactgtgttaagaaatatcgggactgtcccgcgaagagcgggactgtggggcggtatctgaacggggtc
agccgattagcagctctcacatgtgaatgccgggagcctatagttgaacgtcccatcacagccggtagtggctctccctgtgtcctgcagtggcagtcct
tacacgtacgtctttgtactacacgtgtgtgtgtgcgcatctcactctcttaaggggactctttggttttttccttctttttattactattgttattgag
attttatttttaatactgggttagcaaagaggggatttcaaggcggctcaggatttttttctggctccacaggagaatgtctgcgaccagtgttgatcag
agacctaaaggacagggaaacaaagttgcagtacagaacggttcgatgcatcagaaagatgcagtaaacgatgatgactttgagccatatctaaatagcc
aaacgaatcagagtaatagctatccaccaatgtcagacccatacatgcctagttattatgctccatccattggatttccttactctctcggtgaagcagc
atggtcaacagctggtgacccaccaatgccatatttatacggacagatgagcaatggggaacaccactacataccagacggagtgtttagtcagcctggt
gctttaggaaacactcctcccttcctcagccagcatgggtttaatttttttcctggcaatgcagatttctcaacgtgggggacaagtggatctcagggac
aatcaacacaaagttctgcttacagtagtagctacggatacccacctagttctcttggtagagccatagctgatggacaagctggatttgggagtgacgc
tctgagcaaagtgccaggcattaatagcat
3' EST: DT435831 [+]aatcttccacatctaataggacaccataaaatgcataaagaaaaacttaacattgtgaatcgcacatcaacaacttttctaacaagattttagtatttta
aacaattttaaaacaatggaaccttgcattcaatgagtctccagagatttagctaggatgcatgattacaaaaaagacagcagatactggtaaaagcatc
aggacagaaagtgcttgacaaagttaaagttctcaagtgtgtttggtgagagagcatcagggcagatgatgaaaccattatgaggaggggggaaaaaaaa
gtcaattttgtgttaaagtgtccaacgatctcaccaaagcagaaataaaaagcattaaatgtattcttcaacatagtatacgtttccagagtctacatca
ttagtgttgtgattatagcaggatatcgctgtatgattattgtttgtttctattcctctccctacgcatggcttcctcctcttcttgacgcttctcataa
tgtgcaaagtcatcaaagatagaggttgtatgcttgaatgtagcaattattttaagaacttgcttggccttttctaggggtacctcttgagtgtcccttg
agttggtaacaggcttgttatcgttgttttccaaacgaatatgccgcaattggttattaggaacgtctttgacaaagacccatttgacatcaaactttcc
cttccatttgtcctgggaccagactccagcataagcattatagtccacaacagacttcatttcagctacaccacanaaatgtccacttccatttacactg
aagagtaaataaagtgggccttttccattcaaggatcggtaagcagcatccaaacgtttattgccatgttcagtgctg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1562 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: skin | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: