XB-CLONE-3103702
Clone Name: IMAGE:7877194
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Gene:
col12a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT437269
[+]
agatgccggtgacccaggattgccaggaagaatgggttccccaggggtgacaggaccaccagggcctatgggtccaccaggcacaaggggtttcacagga
aaggatggtgcagtagggcccagaggaccaccaggaccaatgggagcacctggacctcagggagtgccaggtccaagtgggaaaccaggaaagcaaggag
ataatggattgccaggtcctcttggacagaaaggagacaagggtgagaggggagacatggcatctcagaatatgatgagatcagtagcaagacaagtctg
tgaacagatgataaatagtcaaatgagcagatttaatcagatgttgaaccaaattccaaatgattactattccaatcgcaatcagcctggtcctccaggt
cctcccggtcctccaggcaacgctggcatgagaggagaacctggagctgctggcagatctggattcccaggaacacctggtcctcaaggaccacctggag
aaagaggaataccaggagagaaaggggagagagggaacccaggaattggatcccaaggacccaggggacttccaggacctccaggacctccaggcgaatc
cagaacaggacctccaggatcctcaggctccacaggacctccaggaccttcaggtcgccccggtaaccaaggaattagagggcctcctggtccagctgga
tactgtgatgcttcaatgtgtgcaagcattccttacaatgggcaaggatacccaggttccggctaacacattttctaagtctccagtgctgcttacagtt
tgaactcatgaaaatcctgtttctgagatgtttgcgcacgtgcttaccttgaaatgattcttttgggaaatttcaccatggatataactaatgacccatg
ttgncatcagtaaaaaggggac
3' EST: DT437268
[+]tttttttttatattttcagtagttttattgaaaaacgcctcttttgtttcagaaataaataaaaatataaggcagtgaaattcacaatttgcagttaaaa
atcttcaaacagcaattctattttcatagccttgcagacaaattcaaactgctttgagaactaagaaacactatattctgaaaaaatgttcatactaaat
atacagcacaaacatgcaaactccttttctgtaggactgactgaaacttggcatacatgtaactgttattgtgtctacaatataagcctgcatcaaaatg
acacagcatacttagctctgtgaaggggttttgctatgttgagaagtttctgaatgttaaaaagaaagtatagtataaaaaaagcagcataacttttatg
aagcataactgaagcctgagtagtacgtgaaagtacagaaggactggaaaatgatagcgcagaaggactgacagactctaaattattacagtcagctgac
aattaaaagataagaaaagcgcaagatgaaagtgcaattcatttctttgtaatgtgctgcatttccatctagcatgcaatgggctaaacctctgcttagg
ttaccagccaatggaaagccttaaattgcatctgcattagagctatttaacattgctacatggtattgcctctattcacagatttcaaattaagccaaaa
ctattttacaagcttatagggggacatcaaacaaagcttcattttttttgttctgttagttaaaattatttccatcattatgtagctgcagtatcctgga
atggaatggcaaaatgtgctatccagctaatctgtgtatatctgcatattcaaatatagccaaggaaattgcagaaatatcttaaaatttctttg
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1562 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: skin | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: