XB-CLONE-3102086
Clone Name: IMAGE:7877817
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Gene:
ptdss2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123999 [+]
GCTTCCTCCAGCCGAGGGCTTCGGCCGCCGCGACGTCACAGGTCACGTTGGTTGGGGCCCCACGTGGTCTGGCGAAAGCGGCTCCGCCCCGCGAATGTTT
TGTGAGCTGCTACCTGTGTGAGGAGCCAGTTCATATGCGAGCCGGGAGGTGCCGGGGAGAGGCTGCGGGACCATGCTGCGTTCCGATGTGAGGAGAGTCG
CCGCTGGAAGAAAAGCTTCCCTGGACGGGCTGTACTGCGAGGAACTGGAGGTCCGGGGAAACAGGAACCTGGGAAATAAGTTCTCTCACAGAGGGGAGCT
CCGCAGGAGCACGGAGTCGGAGGTGTTTGATGATGGCACCAACACTTTCTTCTGGAGAGCTCATACGTTGACCGTGCTATTTATCCTCACGTGTTCACTT
GGATACGTCACCTTACTCGAAGAAACGCCTCAAGATACAGCATACAATGCAAAGAGAGGTATTATTGCCAGCATATTGGTTTTCCTGTGTTTTGGAGTTA
CGCAAGCTAAGGATGGGCCATTTTCAAGACCACATCCAGCCTATTGGAGGTTCTGGCTATGTGTCAGCGTGGTGTATGAGCTTTTCCTCATCTTCATTCT
TTTCCAGACTGTTCACGATGGGCGGCAGTTTATGAAGTTTATCGATCCCAAACTGGGTGTACCGCTACCTGAAAGAGATTATGGTGGGAACTGTTTAATT
TATGACCCTGGCAACAAAACAGACCCTTATCACAATCTCTGGGATAAAATGGATGGCTTTGTTCCAGCACATTTTCTTGGCTGGTATATAAAAACACTAA
TGATAAGAGATTGGTGGATGTGTATGATCATCAGTGTCATGTTTGAATTCTTGGAGTACAGCTTGGAACATCAGCTGCCAAACTTCAGTGAATGCTGGTG
GGATCACTGGATAATGGATGTGTTGGTGTGTAATGGATTTGGAATCTATTGTGGAATGAAGACCCTGGAATGGCTGTCCATGAAACCCTACAAGTGGCAG
GGGCTTTGGAATATCCCCACTTACAGGGGTAAAATGAAGAGAATCGCCTTTCAGTTCACACCTTATAGTTGGGTGAAGTTTGAATGGAAACCTGCTTCGA
GTCTGCGGCGCTGGTTAGCGGTTTGTGGAATCATATTTGTATTTTTATTGGCAGAGCTCAATACTTTTTACCTCAAATTTGTTCTCTGGATGCCACCGGA
ACACTACCTGGTCCTGTTACGCCTTGTCTTTTTTGTGAATGTGGGAGGTGTGGCCATGAGGGAGATCTACGACTTTATGGATGACTTGAAATTCCATAAG
AAGCTGGGGCAGCAGGCATGGATGGTTGCTGCTATTACAGTGACAGAGTTTCTTATTGTTGTGAAATACGACCCGTACACCATAACGTTGCCTTTACCGT
TTTATGTCACACAATGTTGGATTCTGGGTATCGTTCTTGTTCTGACGTGGACAGTGTGGCGCTTCTTCATTAGAGATATTACACTGCGCTACAAAGAAAT
ACGACAGCAAAAACAGCACAGGAATGAAGAGGAAAAATCCCATCGCAATGGGGACGTGAATTCTGAAAAGGACACGAATAAACACAAAAAGCACTAAAAC
AACTTTCTGCCTCTGCGCTCAGCTCCATGTTACTGAACAACAATTGTACTTTTTATATTTTAATGGATACTTCTACTGCTATTTAATAATGAAACGCCTA
AACCTCATTATGTTTTTTTTTTTTTTTGTTTGGTTTTTTTTTCTCCTTTTTTTTCTTGTGGAATATAAACCCGGAGCTTGAAGAGATCAATTCTGCTGAT
TGCACAAGGCAACAATTATGTATTTTCCAATTTGGGAGCCGTGTCCTTATATTTTTTTTTTTTAGTGTCTGAAGAAGAATATTTGCTGTTTTTCATGTAG
CAAGGGTTCTCAGACTGTAAGCCGTGGGGTTATGTATATTTGTTAGATATTTCCAGAAATCCGCTAGTTTATTTAAGGGTTTTTATTAAAATATTTCAGC
AGAAAAAAAAAAAAAAA
5' EST: DT438341 [+]cttcctccagccgagggcttcggccgccgcgacgtcacaggtcacgttggttggggccccacgtggtctggcgaaagcggctccgccccgcgaatgtttt
gtgagctgctacctgtgtgaggagccagttcatatgcgagccgggaggtgccggggagaggctgcgggaccatgctgcgttccgatgtgaggagagtcgc
cgctggaagaaaagcttccctggacgggctgtactgcgaggaactggaggtccggggaaacaggaacctgggaaataagttctctcacagaggggagctc
cgcaggagcacggagtcggaggtgtttgatgatggcaccaacactttcttctggagagctcatacgttgaccgtgctatttatcctcacgtgttcacttg
gatacgtcaccttactcgaagaaacgcctcaagatacagcatacaatgcaaagagaggtattattgccagcatattggttttcctgtgttttggagttac
gcaagctaaggatgggccattttcaagaccacatccagcctattggaggttctggctatgtgtcagcgtggtgtatgagcttttcctcatcttcattctt
ttccagactgttcacgatgggcggcagtttatgaagtttatcgatcccaaactgggtgtaccgctacctgaaagagattatggtgggaactgtttaattt
atgaccctggcaacaaaacagacccttatcacaatctctgggataaaatggatggctttgttccagcacattttcttggctggtatataaaaacactaat
gataagagattggtggatgtgtatgatcatcagtgtcatgttttgattcttggagtacagc
3' EST: DT438340 [+]gctgaaatattttaataaaaacccttaaataaactagcggatttctggaaatatctaacaaatatacataaccccacggcttacagtctgagaacccttg
ctacatgaaaaacagcaaatattcttcttcagacactaaaaaaaaaaaatataaggacacggctcccaaattggaaaatacataattgttgccttgtgca
atcagcagaattgatctcttcaagctccgggtttatattccacaagaaaaaaaaggagaaaaaaaaaccaaacaaaaaaaaaaaaaaacataatgaggtt
taggcgtttcattattaaatagcagtagaagtatccattaaaatataaaaagtacaattgttgttcagtaacatggagctgagcgcaaaggcagaaagtt
gttttagtgctttttgtgtttattcgtgtccttttcagaattcacgtccccattgcgatgggatttttcctcttcattcctgtgctgtttttgctgtcgt
atttctttgtagcgcagtgtaatatctctaatgaagaagcgccacactgtccacgtcagaacaagaacgatacccagaatccaacattgtgtgacataaa
acggtaaaggcaacgttatggtgtacgggtcgtatttcacaacaataagaaactctgtcactgtaatagcagcaaccatccatgcctgctgccccagctt
cttatggaatttcaagtcatccataaagtcgtagatctccctcatggccacacctcccacattcacaaaaaagacaaggcgtaacaggaccaggtagtgt
tccggtggcatcc
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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