XB-CLONE-3101474
Clone Name: IMAGE:7877872
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Gene:
art5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121650 [+]
CCGACATGTCCGGGGTTCCGGCGCCTGGCACCGACACAGGCTGGGAATGGGGAGCCCTGGGCCCGGGATTGTGGCCAACGCTTTACTGAGCCAAATGGAT
CATCTGATGAGGATCTGGCCAATCGTATGGCTCCTGCTCCAGCCCATAATAACGCAGAAAACCCTCTCAGAAGCCCCCATGGATCTGGAAGAAGATACGT
TTGATGACCAGTATATTGGGTGTAGGGACCAAGTGGACCTTCTCATGGATGAGATCCTGAAGACTGAGAAGTCAAAGAATAGATTATTGAGACGCGCTTG
GACAGAAGCAGAGAACATATGGAACATCAGGAGACACGAGATCCAGTCGGAGCTTCCGGTGGGCTTTAGAGACAGCCACGGGGTTGCCTTGGTGGCCTAT
TCTGGGTTCATTAGAACATACTTTGATGAAGCCGTGGTCTCTGCCGGCAAGTCCTACAATGACTACATCCAGCGGTTCCAGTACAAAGGCCTCCATTTCT
ACCTGACGGTGGCCGTGCAGCTCCTGTCTGGGGGGTGCCAGAAGAGACCCAAACTGGTTTATCTTGGGACCAATGGGTTTTGGTACCCAACTAACTCTTT
AGAAGAAGTCAAGTTCGGCCATTTTGTATCTACCTACTCAGAGAAGGAACAAGCGGTGGCCTTTGGTAACGACACCGTCTTTACTATCAGTAGCTGCTAC
GGGGTCCAGGTGGAAAAGTTCTCCCAGTTCCCCCAGGAGAAGGCAGTTCTGGTTCCGAACTATGAGATCTTCTCGGTCCATGAGGGAGAGGTCAATGAGT
TTGCCCTTATCAGCGCCAAACGGACCTGCAGCAAATTCAATTGCGCCCTAATTAGAGGGGAGGGATCACAGCTGAAACCCGGGGAGTGCGCCCCCGACTC
CCGTGCCACACTGAGGATTTCCGACTCAGCGATGCGTGACTTGCCCTTTAATCTGCTTTACATTGCTGTGTCATTCCTGTTGGGCGCCCGGCATTTATTA
CTCATTAGTCTCTAATGTAACGACTTCCCAGCAGGGTGGGGGGGCATGATGGGAAATTGGCTGCAAAACTGTATCTCACTAAAGTGATTTTTATAAAATA
AACTTATATCTGTGCAAAAAAAAAAAAAAAAAA
5' EST: DT438746 [+]ccgacatgtccggggttccggcgcctggcaccgacacaggctgggaatggggagccctgggcccgggattgtggccaacgctttactgagccaaatggat
catctgatgaggatctggccaatcgtatggctcctgctccagcccataataacgcagaaaaccctctcagaagcccccatggatctggaagaagatacgt
ttgatgaccagtatattgggtgtagggaccaagtggaccttctcatggatgagatcctgaagactgagaagtcaaagaatagattattgagacgcgcttg
gacagaagcagagaacatatggaacatcaggagacacgagatccagtcggagcttccggtgggctttagagacagccacggggttgccttggtggcctat
tctgggttcattagaacatactttgatgaagccgtggtctctgccggcaagtcctacaatgactacatccagcggttccagtacaaaggcctccatttct
acctgacggtggccgtgcagctcctgtctggggggtgccagaagagacccaaactggtttatcttgggaccaatgggttttggtacccaactaactcttt
agaagaagtcaagttcggccattttgtatctacctactcagagaaggaacaagcggtggcctttggtaacgacaccgtctttactatcagtagctgctac
ggggtccaggtggaaaagttctcccagttcccccaggagaaggcagttctggttccgaactatgagatcttctcggtccatgagggagaggtcaatgagt
ttgcccttatcagcgccaaacggacctgcagcaaattcaattgcgccctaattagaggggagggatcacagctgaaacccggggagtgcgcccccgactc
ccgtgccac
3' EST: DT438745 [+]gcacagatataagtttattttataaaaatcactttagtgagatacagttttgcagccaatttcccatcatgcccccccaccctgctgggaagtcgttaca
ttagagactaatgagtaataaatgccgggcgcccaacaggaatgacacagcaatgtaaagcagattaaagggcaagtcacgcatcgctgagtcggaaatc
ctcagtgtggcacgggagtcgggggcgcactccccgggtttcagctgtgatccctcccctctaattagggcgcaattgaatttgctgcaggtccgtttgg
cgctgataagggcaaactcattgacctctccctcatggaccgagaagatctcatagttcggaaccagaactgccttctcctgggggaactgggagaactt
ttccacctggaccccgtagcagctactgatagtaaagacggtgtcgttaccaaaggccaccgcttgttccttctctgagtaggtagatacaaaatggccg
aacttgacttcttctaaagagttagttgggtaccaaaacccattggtcccaagataaaccagtttgggtctcttctggcaccccccagacaggagctgca
cggccaccgtcaggtagaaatggaggcctttgtactggaaccgctggatgtagtcattgtaggacttgccggcagagaccacggcttcatcaaagtatgt
tctaatgaacccagaataggccaccaaggcaaccccgtggctgtctctaaagcccaccggaagctccgactggatctcgtgtctcctgatgttccatatg
ttctctgcttctgtccaagcgcgtctcaataatctattctttgacttctcagtcttcaggatctcatccatgagaaggtccacttggtccctacac
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: