XB-CLONE-3101228
|
Clone Name: IMAGE:7878277
|
Gene:
h3-3b
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DT438909 
[+]
cgtaatcaactggtggaaaggctcctcgcaagcagttggccaccaaggcagccaggaagagtgccccctctactggcggtgtgaaaaaaccacatcgtta
taggccaggaactgtagctcttcgtgagatccgtaggtaccagaaatccacagaactccttatccgcaagctgcccttccagcgactggtgagggaaatt
gcccaggatttcaaaactgacctgaggttccagagtgcagccattggtgctttgcaggaggctagcgaagcttacttggttggcttgtttgaggatacca
atctctgtgccatccatgctaagagagtgaccatcatgcccaaggatatccagcttgcccgcagaatacggggagagcgagcatagttggctttcttgta
gtaaattctgtaatatacttaatcttgtgaaatgttttgtatgacctgtttataatgtctccagaaccattccatctgtcactcagggtgaatccttatt
ctgttaatagatgcctcctactacaaagtcactcataaaatggagagatgataggngggtgggtgctcctagcttgtttatacatgatttttgtttattt
gcccccccccccccccccccttctcagcatgtggctagctttttaaaacagcatagtgaaactagagggctgggatcaaaaaatggtgctaaggaccagc
ctttagactaccatatttataaatcaaatatggccttacaggagcaccattgcatatggtgttttgttttctcatacctacgga
3' EST: DT438908 [+]gaatgtaaaacatttaatttaaaacgtcaacactacaatatataaaatagctcttgaaaatacacatttgtgagctgtagctgcctggcttacacattat
ctgctaagcacccaaacactttctcccacaccattaggtaattggcaaagggaccacgccggtaggctttggtactaggaaggggaaaagccaagccata
tttactgctgtaatacttattttactttgcatagtgtaaaatatagaaacagtgtacatatcaactatctggggactgatgctgtattgcttggatgaat
ttaaccacctggcctcaaaagtgggattctaaaagcaccacgttcactttaaaagtgggggcaagtgactagttactagcctataatagtcatgcgccac
tctacaaagggtttattgctcactgtgagccccacatttctttgatgacatcccattaagagagtttgcactagatcagatcatcagcactctgcatagt
aagatttcacaacactgctctaccacacacgaggcaagcaacagggcagctcacactcatgaaagaaatgctctgtgagaaaatgataaaacccttttat
ttttagcttatttaagtgatgtaaaatgttatttaaatagtcatgtgtattgtttgatcaagtcagccatctaaaaagcacaactggccatactgctggt
ttagtattttgcactgtgatgtccagctaacagtccatgattaaaacaaccagtttccaacaggagcaatagggaggaagatcactttctcctccgtagg
tatgagaaaacaaaacaccatatgcaatggtgctcctgtaaggccatatttgatttataaatatggtagtctaaaggctgg
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
