XB-CLONE-3096156
Clone Name: IMAGE:7879794
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT442263 [+]
ttttttttttactgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaattttggttttctacgtt
gtgagaacattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacc
cattttgaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaaga
gattccttcatgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatct
ccaaaactcgacttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctt
tctacgtaaagcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacagg
aatgatttgaagagacaagcacaagtcttgcattacaaaatcccccaacagtgactatgtgtgtcttcaattcctaggataagttcatttggaagttggc
cctttttccagtttcaagaggctagcatgaggcgtacacatttgccaaggcaaattaatactttgaagaactcacgcagcaaatgcaacgcatctgctag
cagtccatttagaagcatttgcggtggacaatggatgggccagattcatcatactcctgcttgctgatccacatctgct
3' EST: DT442262 [+]agctttgcatttctcctcttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgca
aagccggctttgctggagatgatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccagaagga
cagctatgtaggtgatgaagcccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatggagaag
atttggcatcacaccttctacaatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacagagaaa
agatgacacagatcatgtttgagactttcaacaccccagctatgtatgtcgccattcaagctgtgctgtctctgtatgcctctggtcgtaccactggtat
cgtgatggactctggagatggtgtcacccacacagtacccatttatgaaggttatgccttgccccatgctatcctgcgtctggacttggctggtcgtgac
ctgacagactacctcatgaaaatcctgaccgaaagaggctacagcttcaccaccacagctgagagagaaattgtgcgtgacatcaaggagaaactctgct
atgttgctcttgactttgaacaagagatggcaactgctgcatcctcctcatccttggaaaagagctacgaactgcctgatggacaagtaattacaattgg
aaatgagaggtttagatgcccagaggcactcttccagccttccttcctgggtatggaatcatgtggcatccatgaaactacattncactca
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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