XB-CLONE-3090517
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Clone Name: IMAGE:7882369
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT446707 
[+]
tccatcgattcgcgtcactgaggatgaaatgttctccgatatctacaagatcgccgaaacccccgacggcatgtgttttgaagtggaagggaagatcatc
caaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctgagtgccaggaggaggacatcggtggtgcaacaacagtcagcgggg
tggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactcttacaagaattacattaaggattatgtaaaactagtcaaggccaaact
tgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagatagagtaaaactcatacttggaaacttcaaaaactatcagttttacact
ggagaaagaatgaacccagatggtatggtggctctgctagattaccgtgaggatggtgtaacaccttttatgattttctttaaggatggcttgatcagtg
agaaatgttaacattcaatttggttcttccatcttggctgctgcttttcatccatgaacaccaggtctaagcaagatcttactgactcctaaaggactga
tgatacattgagcttgtttcttcatggccctaaattattcaagtggaggcttttttgtttttttaattgaaatccttttgtcatttagagtaaataaaag
cccataattctccactgtagacattgcctcttggtttgatctgcaattacttttgaagactgggtcttgtgcatttaagatttgttgcgcanaagctttg
acttatcagactgactgccagaacattttgtaagaagcggaccttcagtttgtggaca
3' EST: DT446706 [+]acattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgttaacaggcaacagtcaatgttccaa
atccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccagaggtatttgcaacactgttgaaaggccttctggcagtggagga
gtctaaaaacctgaagccgagaggcagattgaatactttggctgtttccttaagtgtgcagattgtccacaaactgaaggtccgcttcttacaaaatgtt
ctggcagtcagtctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaattgcagatcaaaccaagaggcaatg
tctacagtggagaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaacaaaaaagcctccacttgaataatttagggccatga
agaaacaagctcaatgtatcatcagtcctttaggagtcagtaagatcttgcttagacctggtgttcatggatgaaaagcagcagccaagatggaagaacc
aaattgaatgttaacatttctcactgatcaagccatccttaaagaaaatcataaaaggtgttacaccatcctcacggtaatctagcagagccaccatacc
atctgggttcattctttctccagtgtaaaactgatagtttttgaagtttccaagtatgagttttactctatcttgaattcctttcatgaagggttttact
ctgtcgggatcagtctcctcaagtttggccttgactagttttacataatcc
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
