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Summary Attributions
XB-CLONE-3080919

Clone Name: IMAGE:7886017 Gene: cela3a Species: Xenopus tropicalis  
 

Sequence:

5' EST: DT453064 [+]

3' EST: DT453063 [+]

Library:

Name: NIH_XGC_tropSpl1External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Anatomy: spleenStage:
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems