XB-CLONE-3079356
Clone Name: IMAGE:7886302
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Gene:
itpk1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125758 [+]
CACATTCTGTCGCTCAGTGGCAAGAGAAGGAAGGCACCAGGGGAAGGGTGCTAAGGGTTGTAGCAGAGGTTGTGTTCTCGCACTGAAATTCTAACCTCCC
TGTGTGCCCTCCCAGCTTGGAGCATAAGCCGACCAGGGAGGGACCGAGTTCAGCAAATGGAGATGTGAAAGAGGGGGAGGGAAACAAGAGAGAGAGGGAG
GGAGAGACGGACTGATCATTGAAAGGAAGAAGACCGGGACCTGTCTCTTGCACAGACCCTAACCTGGAGCTCAGCATCTACTGATCAGGCATTGTTGCTA
AGACTGCTAAAAATGCAGACCTTCCTCAAAGGGAAGAGAGTGGGTTACTGGATGAGCGAGAAAAAGATCAAGAAACTGAATTTTCAGGCATTTGCAGACA
TGTGCAGGAAACGAGGGATTGAGGTCATACAGCTTAATCTGGCCAAACCTATTGAGGACCAAGGCCCTTTAGATGTGATTATACACAAGCTGACAGACGT
CATTCTTGAGGCTGACCAAAAAGACAGTGAGTCCATCCAGCTGGTGCGAAGGTTCCAGGACTATATAGAAGGCCACCCTGAAACAATACTACTGGACCCG
TTGCCTGCCATTAGAACATTGCTGGACCGCTCAAAATCTTACGAGCTTATTCGCAGAATAGAAACATACATGCAAGATGAAAGGCTGTGTTCCCCTCCAT
TTATGGAGCTAATGGCAGAATGTGATGAAGATACCTTGAAGATTGTAGAGAAGAATGGCTTGGCATTTCCATTAGTTTGTAAGACTCGAGTGGCCCATGG
CACAAACTCTCATGAGATGGCGATTATATTCAATCCAGAAGGACTGCGGTCAATCAAGCCTCCCTGTGTAATTCAAAGCTTCATTAGCCACAACGCAGTC
CTCTACAAAGTCTTTGTGGTGGGAGAATCTTACACAGTAGTGGAGCGGCCATCCTTGAAAAACTTTTCACCTGGAGCTTCTGACAGAGCATCCATATTCT
TCAACAGTCACAATGTGTCAAAGCCTGAATCATCCTCCATTCTAACAGCACTTGACAAGGTGGAAGGAGTGTTTGAGCGCCCCTGTGATGAGGTTATTCG
TGGGATCTCCAAAGCACTACGGCAGGCTCTGGGAATCTCTCTTTTTGGCATTGATATCATTATAAACAACAAGACTGGTCAGCATGCTGTGATTGATATC
AATGCTTTCCCAGGCTATGAGGGTGTCCCAGAATTCTTCACTGACCTCCTAAACCACATTACAACCATCTTGCAGCGCCCAGATCAGAGTGCAAGCAAGT
CCTCAGTGGAGCAGACAGGAAGCTCAATTGGAGAGCGCCTTTGCTGTCCTCCATCTGCCCGAACAGACCCATGGATTGTGGAGACAGACACAAGTGGATC
TGTAAAGTTGCAAAGTCAGAGACTAGGATGCAATTCATCAGTTTCTCCCAGCTTCCAGCAGCACTGTGTGGCCTCTTTGGCAACCAAGGCATCTTCCCAA
TAACAAGTCATTTTTGAAGGACCACAGGGAGTAATAGAATTCCAAAGGGACATGGGAACAGTTTTATTTTGGTGTTCATCGCGTAACTACTTGAACTGAG
TTGTAACAGCAGGTTGTAGGTCGGTTAAAGGGCAGCTGGACAGTTAGTGTTTTAATTGACATCATATTTACAGCTTTTTTCAAAGAAGAAAGTTGGTATA
TTTAATCTTCCCCTGTGCAATTGTGAGAAACTCCACTGTCCAATTTCAAGGGATCCCAATAAGCAGACACTGGCTCTTCATCATTTCAGAGGTTCCCTTC
TGCAGCCATAGGAGGACAGTACTCTTTGCTCAATGTTCTACTAAGACATAGTACAGTCCTTAAAATTGCAACTCTTCAACTCAGACATGTTCCTAAAGTG
GGTTGCGTTCTGAGAGGGAAAAAAAGGAATTATTTTATAACAAAAAACAAATTTTATTCTTTAAAAAAAAAATTACATATTCCTACTCTTTTTTTCACCA
TAAGAAAATGACCACACATCAACCACTGGGTCTTGTGATTGTAGAATTTTAATTCCTTAATTTTAAAAAAAATATTCTTTAGTATTCTGCACCTTGATTT
TTTTTAATCTTGTGATTTAGATTTTATTTGAAACGTTTCAGTCTTAATGTGCTTCCCAAATCTTTAATACCCTTCCCTCAGCTCTCTGCATTCTGTGATA
AGGTGCACAGAGAAAGTCTTAAACAAAAATATTTGCTGCTGTAGACCAAATAAAGGCAGAGCAGTTAGGGGTCACTTCGTTTGGATTTAGCACTATGTGT
CTAATGTAATCTCTTGAATTCAGAGCATTATTCATATATCCAACAGTGCCAGTTGTGTTTTGCTAAAATTCTACCCTTGTGAAAAAGTTCAGAAAGTAAA
ATATAAGGTTTGCTGTGGATCAGAGATTGATAAACTGTGATGAATCTTCAGGCTTTATCCAAGGATGCTGTGGGTTTTAGTTAAGCAGCAGCAGAAGTTT
TGCAGTTTGTCTGGCACTGCTCTACTGGTCTACATATTCCAATAATTAACATTCTACCTTGTAAGTTATTTTGCTGCAGTTAGATTTAAGCACTTATATT
GGATACTGAACAATATTAATTGAGTTTCTAATTTAGTGTGATGATGAGCATACCCTGGCCTGGCAGGTATAAACCAGAATGTTATAGATTACAAAAGTCA
CAATGGGTTTTTTGATGGTGGTGCTTGGTACATTGAAAGTGACAGAAAGAGAAAATCAGAGACAAACCTATACATGTTAGGATCTGCCAATAAAGGAGAA
AATAAAAGTGATCTTTTCTTAAAAATGACTTCTACTTACGTAAGCAAATGATATCTGTAGTTCATGAAAGCATGGGTTGTTATGTAGATGGATAATTGAA
CTGTAACTATATAGTTCTTTTTTTAAAGGAAATCTCAAATCAAGTCCTAGATTTTCTCTGCAGTGCACTTATAGAAGCTAAACTGTCTTACCTAGGTTAC
CCAGTCTTCAAATCATTCCAATTAGATTTACTTTTCTTCATTTCTCCAACAGGGACCCCTTAACACCAGCAAGGATTTAAATGTTCATAACCAAGAGATT
ATTCAGAAAACAAGATTGGCATTATGTCTTACCATACCTCGATTTTGTCCCTTCCTTGTAACATCAGCAATAAAATATTAATTTAATCAAAAAAAAAAAA
AAAAAA
5' EST: DT454097 [+]cacattctgtcgctcagtggcaagagaaggaaggcaccaggggaagggtgctaagggttgtagcagaggttgtgttctcgcactgaaattctaacctccc
tgtgtgccctcccagcttggagcataagccgaccagggagggaccgagttcagcaaatggagatgtgaaagagggggagggaaacaagagagagagggag
ggagagacggactgatcattgaaaggaagaagaccgggacctgtctcttgcacagaccctaacctggagctcagcatctactgatcaggcattgttgcta
agactgctaaaaatgcagaccttcctcaaagggaagagagtgggttactggatgagcgagaaaaagatcaagaaactgaattttcaggcatttgcagaca
tgtgcaggaaacgagggattgaggtcatacagcttaatctggccaaacctattgaggaccaaggccctttagatgtgattatacacaagctgacagacgt
cattcttgaggctgaccaaaaagacagtgagtccatccagctggtgcgaaggttccaggactatatagaaggccaccctgaaacaatactactggacccg
ttgcctgccattagaacattgctggaccgctcaaaatcttacgagcttattcgcagaatagaaacatacatgcaagatgaaaggctgtgttcccctccat
ttatggagctaatggcagaatgtgatgaagataccttgaagattgtagagaagaatggcttggcatttccattagtttgtaagactcgagtggcccatgg
cacaaactctcatgagatggcgattatattcaatccagaaggactgcgg
3' EST: DT454096 [+]gattaaattaatattttattgctgatgttacaaggaagggacaaaatcgaggtatggtaagacataatgccaatcttgttttctgaataatctcttggtt
atgaacatttaaatccttgctggtgttaaggggtccctgttggagaaatgaagaaaagtaaatctaattggaatgatttgaagactgggtaacctaggta
agacagtttagcttctataagtgcactgcagagaaaatctaggacttgatttgagatttcctttaaaaaaagaactatatagttacagttcaattatcca
tctacataacaacccatgctttcatgaactacagatatcatttgcttacgtaagtagaagtcatttttaagaaaagatcacttttattttctcctttatt
ggcagatcctaacatgtataggtttgtctctgattttctctttctgtcactttcaatgtaccaagcaccaccatcaaaaaacccattgtgacttttgtaa
tctataacattctggtttatacctgccaggccagggtatgctcatcatcacactaaattagaaactcaattaatattgttcagtatccaatataagtgct
taaatctaactgcagcaaaataacttacaaggtagaatgttaattattggaatatgtagaccagtagagcagtgccagacaaactgcaaaacttctgctg
ctgcttaactaaaacccacagcatccttggataaagcctgaagattcatcacagtttatcaatctctgatccacagcaaaccttatattttactttctga
actttttcacaagggtagaattttagcaaaacacaactggcactgtt
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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