XB-CLONE-3064038
Clone Name: IMAGE:7845639
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT527897 [+]
tgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatat
gaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctggatggaacggtgacaaca
tgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctggaagctcttgactg
cattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtaccagttggtcgtgtg
gagactggtgtccttaagccaggcatggtggttacttttgcccctgttaatgtaacaactgaagttaaatctgttgaaatgcaccatgaagcccttagcg
aggccatgcccggtgacaatgttggctttaacgtgaaaaacgtttctgtgaaggacgtccgtcgtggcaacgttgctggtgacagcaagaatgacccacc
aatggaagctggtacctttacagcacaggttatcatcctgaaccacccaggccagattggtgctggatatgcccctgtgttggattgccacacagctcac
attgcttgcaagtttgctgagctgaaggaaaagattgatcgccgttctggtaagaaactggaagacaatcccaagttcctgaagtctggtgatgctgcca
ttgttgacatgatcccaggaaagcctatgtgcgtggagagcttctctgactac
3' EST: DT527896 [+]ttttttgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctgagaccaatcctcca
ccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagctccagcagccttct
tatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacat
aggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttcc
ttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctgggtggttcaggatgataacct
gtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcacagaaacgtttttcacgttaaa
gccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacattaacaggggcaaaagtaaccacc
atgcctggcttaaggacaccagtctccacacgaccaactggtacagtaccaataccgccaattttgtagacatcctgcagaggcagacgcagaggcttat
cagttgggcgacttggtggcaaaatgcagtcaagagcttccagcagggtagttccgctgccagatcccctcttacgtgaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: