XB-CLONE-3063972
Clone Name: IMAGE:7845645
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Gene:
glipr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT527940 [+]
ctttggccgacccgggttccgcagtaacacagggcactttactcaggttgtctggaaggactctaaggaggttggagttggggtggccacagacggcaat
ggtctttatttcgtggtgggacagtacaacccagcggggaatatcaccaaccccggatactttgagaagaacgttctgcccgccgggtcatctccaggac
cagatagtgccgagtctggcagctcccagtggaaaccagcaggaggctacagagaaccaacaggaggctacagagaaccaacaggaggctacagagaacc
aacaggaggctacagagaaccagcaggaggctttgtagaaccgcccagaagcttcagagaacctgaaaagacatctagcaacttcaaacccgtatcggga
ttaaccaagcagggagctgggagttttgagcaggaagctctggacgcgcacaataagtatcgacgccaacatggcgcaccccaactccaactctcccggg
agctgtgcgactccagccagaagtgggccgaccacctactctccatcaacgccctgcagcacagcaacaccgccaacggggagaacctgtggtacaagtg
gaactccagcatgagggatgcctctggtgcggaggtggtggaaacgtggtacaacgagattaaggattatagctttggccggccgggcttccagtctaac
acaggtcactttacacaggttgtctggaaagactccagagaggttggcgttgccaaagctgtggatggcaaaggaatggtgatagcggtggcgcagtaca
gcccagctggcaacatcaccaaccccggctacttccagaagaacgttctgcc
3' EST: DT527939 [+]ggaggatgttgcggccgtagaatcccgggttggtgatgttcccactggggttatactgggccaccacaatcaacatccctttgccgctggaggccagacc
aacacccacctccttggaggctttccacaccatctgggtgaaatgacctgtgtcggcctggtatccgggcttggagaagttgtaattcttctcttctttg
taccagctgtcggccacttcttgccctgtgacggtgatggaggggcctccagatttagcccagatattctccccatgggacgtgtcactgtgctttaaag
tcttcaggttgaggagatgctccgcccaccgttgggcttcctgacttatcttcgagttgagctgcagcggttttgccccgtggcgtgatcgataaacgtt
attggccttcaggaactcaagggcaaattctctggaatctgtaggtgacgaggccgagtctgttccgcgtgttcctagatacgaggttcccctggcggtg
gggcttgttcccgtatcggatacaggagttcccttcggcagaacgttcttctggaagtagccggggttggtgatgttgccagctgggctgtactgcgcca
ccgctatcaccattcctttgccatccacagctttggcaacgccaacctctctggagtctttccagacaacctgtgtaaagtgacctgtgttagactggaa
gcccggccggccaaagctataatccttaatctcgttgtaccacgtttccaccacctccgcaccagaggcatccctcatgctggagttcacttgtaccaca
ggttctccccgttggcggtgttgctgtgctgcagggcgttgatggagagtaggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: