XB-CLONE-3063670
Clone Name: IMAGE:7845719
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Gene:
psmb4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528141 [+]
cggccggctcctggagagctgcattccttccccggggcggctccggtcagacataccctgaaccctatggtgacgggaacctctgtgctgggggtgaaat
ttgatgggggagtgattatcgcggcggacatgctgggttcctacgggtcgctggccaggttccggaacatctccaggatcatgaaagtgaatgagtcgac
catcctgggggcttccggcgactacgccgattatcagtacctcaaacaggtcatcgaccagatggtgattgacgaggagctggtgggggacgggcacaat
tacagccccaaggccgttcattcgtggctcacccgcgtcatgtacaaccggaggagcaagatgaaccccctgtggaacaccgtggtcatcgggggcttct
acaatggagagagtttccttggatacgtcgacaaactgggcgtggccttcgaagcaccaacgattgctacgggctttggcgcgtacctggcacagccgct
gctgagggaagtgactgagaggaaagcgacgctgagcaaggaggaggcgcgggcgctcatagagcgatgcatgaaggtcctgtactaccgagacgcccga
tcctacaaccggtttgagatcgccacggtaactgaaagcggagtcgaggtcgaggggcctttgtcgtcagaaaccaactgggaaattgctcacctcatca
gcggcttcgagtgaccccgggcacgcgtttcgctccatcatcactttcttattggttttttttttcctctcgcttctttaacgacattggctgttttgta
aatgaatattaaataa
3' EST: DT528140 [+]gacggaaggagccaatcatctcccattatttaatattcatttacaaaacagccaatgtcgttaaagaagcgagaggaaaaaaaaaaccaataagaaagtg
atgatggagcgaaacgcgtgcccggggtcactcgaagccgctgatgaggtgagcaatttcccagttggtttctgacgacaaaggcccctcgacctcgact
ccgctttcagttaccgtggcgatctcaaaccggttgtaggatcgggcgtctcggtagtacaggaccttcatgcatcgctctatgagcgcccgcgcctcct
ccttgctcagcgtcgctttcctctcagtcacttccctcagcagcggctgtgccaggtacgcgccaaagcccgtagcaatcgttggtgcttcgaaggccac
gcccagtttgtcgacgtatccaaggaaactctctccattgtagaagcccccgatgaccacggtgttccacagggggttcatcttgctcctccggttgtac
atgacgcgggtgagccacgaatgaacggccttggggctgtaattgtgcccgtcccccaccagctcctcgtcaatcaccatctggtcgatgacctgtttga
ggtactgataatcggcgtagtcgccggaagcccccaggatggtcgactcattcactttcatgatcctggagatgttccggaacctggccagcgacccgta
ggaacccagcatgtccgccgcgataatcactcccccatcaaatttcacccccagcacagaggtttcccgtcaccatagggttcagggtatgtctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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