XB-CLONE-3063488
Clone Name: IMAGE:7845688
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Gene:
ciao2b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528262 [+]
cgcgaatccgctcatttaccggcgggcgggggagcggcaggtcacagcgcaggaggaggatgaggacgcgccggaccggatcgatgaccgggagatcttt
gatctgattcgctgtataaatgacccggaacacccacttacgctggaggaactaaacgtggttgaagaaattcgagtgaaggtaagtgatgaggagagca
cagtgtccgtcgagtttaccccgaccatcccgcactgcagtatggcaacgctcatcggcctctccattaaagtaaagctcctgcgctctctgcccgagag
gtttaaggtggacgtccatataactcccggcacgcacgcctctgagcacgcagtgaacaagcagttggccgacaaggagcgcgtggccgccgccctagag
aattcccatttgctggaagtcgtcaaccaatgcttatccggcagatcctctgcttaaatcagccgttctgctggggggttatgggtacccagcatgcact
cctgccgctacatgtgacctttaaacaggaaaaggcaaagtcaggcccggactgggatttagaatagaccctgacatatcaagtacaccgaggcccaaac
agcccccccaccggcccaataaatagtgactgcagcccctctggcattggccagaacccacagattgccagtccgggcctgggcagagactcagtccatt
cagggtgtcactgtaatgtcccagtcatgttctgctctgtgtgggtggattagtgatgtgtaggccaacagataaccggcccccgccccacaacccattt
tcaactaggctgcanatcagcgtctgtttacatagaccccgcc
3' EST: DT528261 [+]tttttcaaaataacaaaaacactttaatctcaatgaaaataagtccagttcctatcattattatactcgatccatttagggtgaaacattaatttcccag
tcctgttctggaaatagtggtgcgtatgccccacccacaatatatattatatacacattataacagcctgctctgtattaatgatgtgtggacctcccaa
aacccgtgggggtcggcacttgcacaacatattcaggttgggggcgggtttaagctgagctattcccacccacgacctaacggtaccccctctgccccac
ccccttcatgacatcagagatggggaaggcggggtctatgtaaacagacgctgatttgcagcctagttggaaatgggtttgtggggcgggggccggttat
ctgttggcctacacatcactaatccacccacacagagcagaacatgactgggacattacagtgacaccctgaatggactgagtctctgcccaggcccgga
ctggcaatctgtgggttctggccaatgccagaggggctgcagtcactatttattgggccggtgggggggctgtttgggcctcggtgtacttgatatgtca
gggtctattctaaatcccagtccgggcctgactttgccttttcctgtttaaaggtcacatgtagcggcaggagtgcatgctgggtacccataacccccca
gcagaacggctgatttaagcagaggatctgccggataagcattggttgacgacttccagcanatgggaattctctagggcggcggccacgcgctccttgt
cggccaactgcttgttcactgcgtgctcagaggcgtgcgtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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