XB-CLONE-3063461
Clone Name: IMAGE:7845738
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Gene:
homer1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528280 [+]
gggagagcctctgtcttcatcttggacaactatcctgagaccaaatgagattcagttcttctgctttctcccctacagcgagcaacccatctttagcacc
agagcccatgtcttccagatagaccccaacactaagaagaactgggttccaaccagcaagcatgccgtcacagtgtcctatttttatgatagcacaagaa
atgtctacaggataataagtctggatggatcaaaggcaataataaacagcaccatcagtccaaacatgacattcacaaagacatcccagaagtttgggca
gtgggcagacagccgggctaatacagtctatggcctgggattctcctctgaacatcatctcactaaatttgcagaaaaatttcaggaatttaaagaagca
gctcgattagcaaaggagaaatcccaggaaaagatggaattaactagtacgccttcccaggagtctgctggaggagacctccaatcaccgttaacaccag
aaagcatcaatggaacggacgatgaaaggacaactccagatgtgacgccaaactccgaaccaagggctgaacctacgcagaacgcattgccatttacaca
cagttctgcaatcaacaagcattgggaagcagaactggctacacttaaaggaaataatgccaaactcacagcagccttgctggagtctactgccaatgtg
aaacaatggaagcaacagcttgcggcctatcaagaggaagcagagcgtctacacaagcgggtgagcgaattagaatgtctgagcaatcaagctaatgctg
tgcagtctcacaagacagaactgaaccagaagatacaagaattagaaaccacgct
3' EST: DT528279 [+]gttgtttttaatttccccctctttttttaactagttcagtcttaattttatacttcacccgactaaaaattttgcaacccattctggttttgtgcaactt
ggaggcagagcccatcttggtgctgtcacgagagacagtgcatctttaagaagagggtaagccattggcgctgctggtctctttctagctgcactctatc
agtttggccaggttgtctcgcagttcggtgagttcaaagatttttccatccaggatttctaaaagtgtcttcaagttattgcggaaagcgtcctgctcgt
tctgacttttctccaaccgttgctctaagtcacttaactgtccttccatgtctttgttatgggtttctacctcctgtaatttttggctcaaagaatcttt
ctggtcttgcaactctttagcatgttcaacctcttctttcaatctttctatctcctcttctttcttcttcagcgtggtttctaattcttgtatcttctgg
ttcagttctgtcttgtgagactgcacagcattagcttgattgctcagacattctaattcgctcacccgcttgtgtagacgctctgcttcctcttgatagg
ccgcaagctgttgcttccattgtttcacattggcagtagactccagcaaggctgctgtgagtttggcattatttcctttaagtgtagccagttctgcttc
ccaatgcttgttgattgcagaactgtgtgtaaatggcaatgcgttctgcgtaggttcagcccttggttcggagtttggcgtcacatctggagttgtcctt
tcatcgtccgttcattgatgctttctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: