XB-CLONE-3063437
Clone Name: IMAGE:7845740
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Gene:
ubb
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT528296
[+]
gaagatggccgcaccnctttctgactacaacatccagaaagaatccaccctgcatcttgtccttcgtctgagaggtggaatgcagatctttgtaaaaacc
ctgactggcaagaccatcaccctggaggtagagccaagtgacacaatagagaatgtcaaagcaaagatccaagacaaagaaggtatccccccagaccagc
agaggttgatctttgcaggcaagcagctggaagatggccgcacactttctgactataatatccagaaagaatccaccctgcatcttgtccttcgtctgag
aggtggaatgcagatctttgtaaaaaccctgactggcaagaccatcaccctggaggtagagccaagtgacacaatagagaatgtcaaagcaaagatccaa
gacaaagaaggtatccctccagaccagcagaggttgatctttgccggcaagcagctggaagatggccgcacactttctgactataatatccagaaagaat
ccaccctgcatcttgtccttcgtctgagaggtggaatgcagatccttgtaaaaaccctgactggcaagaccatcaccttggaggtagagccaagtgacac
aatagagaatgtcaaagcaaagatccaagacaaagaaggtatccctccagaccagcagaggttgatctttgccggcaagcagctggaagatggccgcacc
ctttctgactacaacatccaaaaagaatccaccctgcatcttgtccttcgtctgagaggtggttgttaaagtgaaaaaagactttgcttcatgtgatttg
tttgttcacg
3' EST: DT528295
[+]gagaaataaatgctttatttagaacatacaagattactgttaaggaacgtgaacaaacaaatcacatgaagcaaagtcttttttcactttaacaaccacc
tctcagacgaaggacaagatgcagggtggattctttttggatgttgtagtcagaaagggtgcggccatcttccagctgcttgccggcaaagatcaacctc
tgctggtctggagggataccttctttgtcttggatctttgctttgacattctctattgtgtcacttggctctacctccaaggtgatggtcttgccagtca
gggtttttacaaggatctgcattccacctctcagacgaaggacaagatgcagggtggattctttctggatattatagtcagaaagtgtgcggccatcttc
cagctgcttgccggcaaagatcaacctctgctggtctggagggataccttctttgtcttggatctttgctttgacattctctattgtgtcacttggctct
acctccagggtgatggtcttgccagtcagggtttttacaaagatctgcattccacctctcagacgaaggacaagatgcagggtggattctttctggatat
tatagtcagaaagtgtgcggccatcttccagctgcttgcctgcaaagatcaacctctgctggtctggggggataccttctttgtcttggatctttgcttt
gacattctctattgtgtcacttggctctacctccagggtgatggtcttgccagtcagggtttttacaaagatctgcattccacctctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: